Translation Initiation: Cell Biology, High‐Throughput Methods, and Chemical‐Based Approaches

Content: 
Purification of FLAG‐Tagged Eukaryotic Initiation Factor 2B Complexes, Subcomplexes, and Fragments from Saccharomyces cerevisiae Review Article
Pages 1-13
Sarah S. Mohammad‐Qureshi, Raphaël Haddad, Karren S. Palmer, Jonathan P. Richardson, Edith Gomez, Graham D. Pavitt

In Vivo Deletion Analysis of the Architecture of a Multiprotein Complex of Translation Initiation Factors Review Article
Pages 15-32
Klaus H. Nielsen, LeoЕЎ ValГЎЕЎek

An Approach to Studying the Localization and Dynamics of Eukaryotic Translation Factors in Live Yeast Cells Review Article
Pages 33-45
Susan G. Campbell, Mark P. Ashe

In Vitro and Tissue Culture Methods for Analysis of Translation Initiation on the Endoplasmic Reticulum Review Article
Pages 47-60
Samuel B. Stephens, Christopher V. Nicchitta

Mammalian Stress Granules and Processing Bodies Review Article
Pages 61-81
Nancy Kedersha, Paul Anderson

Methods to Analyze MicroRNA‐Mediated Control of mRNA Translation Review Article
Pages 83-111
Jennifer L. Clancy, Marco Nousch, David T. Humphreys, Belinda J. Westman, Traude H. Beilharz, Thomas Preiss

Methods for Studying Signal‐Dependent Regulation of Translation Factor Activity Review Article
Pages 113-142
Xuemin Wang, Christopher G. Proud

Analysis of mRNA Translation in Cultured Hippocampal Neurons Review Article
Pages 143-162
Yi‐Shuian Huang, Joel D. Richter

Detecting Ribosomal Association with the 5′ Leader of mRNAs by Ribosome Density Mapping (RDM) Review Article
Pages 163-175
Naama Eldad, Yoav Arava

Genome‐Wide Analysis of mRNA Polysomal Profiles with Spotted DNA Microarrays Review Article
Pages 177-201
Daniel Melamed, Yoav Arava

Synthesis of Anti‐Reverse Cap Analogs (ARCAs) and their Applications in mRNA Translation and Stability Review Article
Pages 203-227
Ewa Grudzien‐Nogalska, Janusz Stepinski, Jacek Jemielity, Joanna Zuberek, Ryszard Stolarski, Robert E. Rhoads, Edward Darzynkiewicz

Methods for Identifying Compounds that Specifically Target Translation Review Article
Pages 229-267
Letizia Brandi, Attilio Fabbretti, Pohl Milon, Marcello Carotti, Cynthia L. Pon, Claudio O. Gualerzi

Identifying Small Molecule Inhibitors of Eukaryotic Translation Initiation Review Article
Pages 269-302
Regina Cencic, Francis Robert, Jerry Pelletier

Isolation and Identification of Eukaryotic Initiation Factor 4A as a Molecular Target for the Marine Natural Product Pateamine A Review Article
Pages 303-324
Woon‐Kai Low, Yongjun Dang, Tilman Schneider‐Poetsch, Zonggao Shi, Nam Song Choi, Robert M. Rzasa, Helene A. Shea, Shukun Li, Kaapjoo Park, Gil Ma, Daniel Romo, Jun O. Liu

Series Editors
Page II

Table of Contents
Pages V-IX

Contributors to Volume 431
Pages XI-XV

Preface
Page XVII
Jon Lorsch

Contents of Previous Volumes
Pages XIX-XLIV

Author Index
Pages 325-341

Subject Index
Pages 343-348