Transgenesis Techniques: Principles and Protocols Methods in Molecular Biology Vol 18

CHAPTER 1 --
 Introduction to Transgenesis......Page 1
CHAPTER 2 -- Transgenic Rodents and the Study of the Central Nervous System
......Page 5
CHAPTER 3 -- Transgenic Animals and the Study of Cancer
......Page 21
CHAPTER 4 -- Transgenic Animals and the Study of the Immune System
......Page 35
CHAPTER 5 -- The Commercial and Agricultural Applications of Animal Transgenesis
......Page 51
CHAPTER 6 -- P Element-Mediated Germ-Line Transformation of Drosophila  
......Page 70
CHAPTER 7 -- Gene Transfer by Microinjectionin the Zebra Fish Brachydanio rerio
......Page 82
CHAPTER 8 -- Transgenic Induction in Salmonid and Tilapia Fish
......Page 90
CHAPTER 9 -- An Overview of Transgenic Mouse Production 
......Page 103
CHAPTER 10 -- Transgene Design
......Page 107
CHAPTER 11 -- Isolation of DNA Fragments for Microinjection
......Page 111
CHAPTER 12 -- Establishing a Colony for Efficient Production of Transgenic Mice
......Page 117
CHAPTER 13 -- Mating Mice
......Page 123
CHAPTER 14 -- Anesthetizing Mice 
......Page 127
CHAPTER 15 -- Vasectomizing a Mouse
......Page 129
CHAPTER 16 -- Preparation of Culture Media for Fertilized One-Cell Mouse Eggs
......Page 133
CHAPTER 17 -- Collection of Fertilized One-Cell Mouse Eggs for Microinjection
......Page 137
CHAPTER 18 -- Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs -- I. Manual Injection 
......Page 143
CHAPTER 19 -- Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs -- 
II. Automatic Injection......Page 155
CHAPTER 20 -- Delivery of Microinjected Eggs to Surrogate Mothers by Oviduct Transfer
......Page 161
CHAPTER 21 -- Caesarean Section and Fostering
......Page 169
CHAPTER 22 -- In vitro Fertilization of Mouse
......Page 171
CHAPTER 23 -- The In Vitro Isolation of Murine Embryonic Stem Cells
......Page 175
CHAPTER 24 -- Production of Chimeras Derived from Murine Embryonic Stem Cells
......Page 209
CHAPTER 25 -- Cryopreservation of Transgenic Mouse Lines
......Page 231
CHAPTER 26 -- Introduction to the Physiology and Husbandry of the Rat
......Page 236
CHAPTER 27 -- Mating of Rats
......Page 242
CHAPTER 28 -- Vasectomy of the Male Rat
......Page 246
CHAPTER 29 -- Harvest of Fertilized One-Cell Rat 
......Page 250
CHAPTER 30 -- Implantation of Microinjected Eggs
......Page 254
CHAPTER 31 -- Cryopreservation of Transgenic Rat Lines
......Page 258
CHAPTER 32 -- Production of Transgenic Sheep
......Page 260
CHAPTER 33 -- Cryopreservation of Transgenic Sheep Lines
......Page 292
CHAPTER 34 -- Isolation of Genomic DNA from Tail Tissue
......Page 294
CHAPTER 35 -- Slot Blotting of Genomic Tail DNA
......Page 298
CHAPTER 36 -- Genomic Analysis of Transgenic Animals by the Polymerase Chain Reaction
......Page 302
CHAPTER 37 -- Genomic Analysis of Transgenic Animals -- 
Southern Blotting......Page 308
CHAPTER 38 -- The Preparation of RNA from the Tissues of Transgenic Animals
......Page 313
CHAPTER 39 -- Northern Blotting
......Page 319
CHAPTER 40 -- RNA Slot Blotting
......Page 323
CHAPTER 41 -- Analysis of Gene Expression by PCR
......Page 327
CHAPTER 42 -- Nuclear Run-On Analysis of Transcription
......Page 337
CHAPTER 43 -- S1 Nuclease Protection Mapping
......Page 345
CHAPTER 44-- Primer Extension
......Page 355
CHAPTER 45 -- In Situ Hybridization Analysis of Transgenic Tissues
......Page 361
CHAPTER 46-- Immunohistochemical Analysis of Transgene Expression
......Page 377
CHAPTER 47 -- Postmortem Examination of Transgenic Mice 
......Page 389
CHAPTER 48 -- Histological Examination of Transgenic Mice 
......Page 395
CHAPTER 49 -- Reporter Enzyme Assays
......Page 401
CHAITER 50 -- Restriction Endonuclease Digestion of DNA
......Page 407
CHAPTER 51 -- Agarose Gel Electrophoresis
......Page 413
CHAPTER 52 -- Working with Radioactivity in the Transgenic Animal Laboratory
......Page 419
CHAPTER 53 -- Random Primed Labeling of DNA 
......Page 425
CHAPTER 54 -- Filter Hybridization
......Page 429
CHAPTER 55 -- DNA Transfection
......Page 433