Plant-Microbe Interactions: Laboratory Techniques

Front Matter ....Pages i-xxiii
Front Matter ....Pages 1-1
 Isolation, Characterization, and Preservation of Rhizobia from Root Nodules of Legumes  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 3-8
 Enumeration of Rhizobia: Most-Probable-Number (MPN) Technique  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 9-27
 Isolation and Enumeration of Azospirillum by Most-Probable-Number (MPN) Method  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 29-31
 Quantitative Estimation of Leghemoglobin Content in Legume Root Nodules  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 33-35
 Quantitative Estimation of Nitrogenase Activity: Acetylene Reduction Assay  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 37-39
 Quantitative Estimation of Nitrogen Fixation: 15N2 Natural Abundance Study  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 41-43
 Estimation of Glutamine Synthetase (GS), Glutamate Synthase (GOGAT), and Glucose Dehydrogenase (GDH)  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 45-47
 Colorimetric Analysis of Ureide-N, Amino-N, and Nitrate-Nitrogen  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 49-52
Front Matter ....Pages 53-53
 Isolation of Mineral Phosphate Solubilizing Microbes from Rhizosphere Soil  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 55-57
 Estimation of Phosphate Solubilizing Capacity of Microorganisms  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 59-60
 Isolation, Enumeration of Spores, and Inoculum Production of Arbuscular Mycorrhizal (AM) Fungi  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 61-63
 Isolation of Zn Solubilizing Bacteria  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 65-66
 Quantitative Estimation of Zn Solubilization Efficiency of Microorganisms  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 67-68
 Isolation of Sulfur-Oxidizing Bacteria  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 69-70
 Enrichment Isolation of Sulfur-Oxidizing Bacteria  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 71-72
 Quantitative Estimation of Sulfate Produced by Sulfur-Oxidizing Bacteria  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 73-75
Front Matter ....Pages 77-77
 Enrichment Isolation of 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Deaminase Producing Bacteria  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 79-80
 Estimation of ACC Deaminase Activity in Bacterial Cells  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 81-83
 PCR-Based Detection of ACC Deaminase Producing Bacteria  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 85-87
 Classical Triple Response Bioassay for Determining Stress Mitigating Potential of ACC Deaminase Producing Bacteria  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 89-90
 Estimation of Ethylene in Plant-Bioassay System: Gas Chromatography  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 91-93
 Estimation of Proline Content in Plant Tissues  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 95-98
 Determination of Glycine Betaine by Periodide Method  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 99-100
 Estimation of Betaine Aldehyde Dehydrogenase (BADH) Activity  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 101-102
 Estimation of Malondialdehyde (MDA) by Thiobarbituric Acid (TBA) Assay  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 103-105
 Estimation of Mono-Dehydroascorbate Reductase (MDAR) Activity  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 107-108
 Determination of Glutathione Activity  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 109-111
 Estimation of Catalase  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 113-115
 Estimation of Superoxide Dismutase (SOD)  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 117-118
 Estimation of Ascorbate Peroxidase (APX)  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 119-121
 Estimation of Peroxidase (POD)  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 123-125
 Estimation of Choline Content in Plant Tissues  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 127-128
 Detection of Biofilm Forming Activity of Microorganisms  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 129-133
 Extraction of Extracellular Polymeric Substances (EPS) from Bacteria  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 135-137
 Microscopic Technique for Studying Biofilm Formation  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 139-140
 Determination of Carotenes  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 141-143
 Determination of Chlorophyll  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 145-146
 Determination of Chlorophyll Fluorescence  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 147-148
 Determination of Gas Exchange Measurements  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 149-150
 Drought Tolerance Efficiency  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 151-152
 Estimation of Total Phenol Content of Plant Tissues  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 153-154
 Measurement of Electrolyte Leakage  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 155-156
 Determination of Physiological Traits Related to Abiotic Stresses  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 157-163
Front Matter ....Pages 165-165
 Isolation of Bacteria with Biocontrol Activity Against Phytopathogens: Dual Plate Assay  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 167-169
 Antagonistic Activity of Volatile Compound of Bacteria Against Phytopathogens: Dual Plate Assay  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 171-173
 Detection of Pyoluteorin by Thin Layer Chromatography  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 175-176
 Detection of Siderophore Producing Microorganisms  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 177-181
 Quantitative Estimation of Siderophore Production by Microorganisms  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 183-186
 Extraction and Characterization of Siderophore [Catecholate Type] by Thin Layer Chromatography  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 187-189
 Purification of Siderophores by High Performance Liquid Chromatography and Electrospray Ionization-Mass Spectrometry (ESI-MS)  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 191-192
 Isolation of Microorganisms Producing Hydrolytic Enzymes: Protease  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 193-194
 Isolation of Microorganisms Producing Hydrolytic Enzymes: Lipase  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 195-196
 Isolation of Bacteria Producing Thermo Stable Hydrolytic Enzyme: Chitinase  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 197-198
 Isolation of Bacterial Hydrolytic Enzyme: Chitinase  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 199-200
 Isolation of Fungal Hydrolytic Enzyme: Chitinase  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 201-202
 Characterization of Chitinase Enzyme from Streptomyces  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 203-204
 Chitinase Assay by Spectrophotometric Method  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 205-205
 Extraction of Fungal Chitinase Enzyme  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 207-208
 Partial Purification of Bacterial Chitinase Enzyme by Ion Exchange Chromatography  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 209-210
 Isolation of Actinomycetes Producing Hydrolytic Enzyme: β-1,3 Glucanase  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 211-212
 Isolation of Bacteria Producing Hydrolytic Enzyme: β-1,3 Glucanase  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 213-214
 Purification of Trichoderma Producing Hydrolytic Enzyme: β-1,3 Glucanase  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 215-216
 Estimation of Lipoxygenase  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 217-218
Front Matter ....Pages 219-219
 Selective Isolation of Spore Forming Bacillus  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 221-223
 Spore Staining and Biochemical Characterization of Bacillus  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 225-229
 Insect Bioassay for Determining LD50 of Crystal Protein  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 231-234
 Insect Bioassay for Determining LD50 of Spore–Crystal Protein Mixture  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 235-237
 Purification and Characterization of Crystal Proteins  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 239-241
 Purification of Crystal Proteins Using of Organic Solvent  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 243-244
 Purification and Characterization of Vips from Culture Supernatant of Bacillus  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 245-248
 Detection of Vegetative Insecticidal Protein (Vip) in Bacillus  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 249-251
 Selective Isolation of Entomopathogenic Fungi  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 253-255
 Galleria Bait Technique for the Isolation of Entomopathogenic Fungi from Soil  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 257-259
 Microscopic Characterization of Entomopathogenic Fungi  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 261-264
 Isolation and Characterization of Nematode Species with Economic Importance  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 265-269
 Isolation of Nematodes from Soil Sample  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 271-273
 Extraction of Nematodes from Plant Materials  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 275-277
 Techniques for Maintaining Pure Nematode Population  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 279-282
 North Carolina Host Differentiation Test for Identification of Nematode Population  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 283-284
 Isolation and Characterization Nematode-Egg Parasitic Fungi  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 285-287
 Assessment of Nematicidal Activity of Microbial Volatiles  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 289-291
 Trap Method for Screening Nematode Trapping Fungi  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 293-294
 Direct Observation Method for Identifying Pasteuria: An Obligate Parasite of Nematodes  (M. Senthilkumar, N. Amaresan, A. Sankaranarayanan)....Pages 295-296