Oxygen Radicals in Biological Systems

Content: 
Contributors to volume 103
Pages ix-xiii

Preface
Page xv
P.Michael Conn

Volume in series
Pages xvii,xix-xxvi

[1] Synthesis and use of neuropeptides Original Research Article
Pages 3-32
John M. Stewart

[2] Preparation of radiolabeled neuroendocrine peptides Original Research Article
Pages 32-48
Richard N. Clayton

[3] Ligand dimerization: A technique for assessing receptor-receptor interactions Original Research Article
Pages 49-58
P.Michael Conn

[4] Photoaffinity labeling in neuroendocrine tissues Original Research Article
Pages 58-71
Eli Hazum

[5] Microisolation of neuropeptides Original Research Article
Pages 72-89
Fred S. Esch, Nicholas C. Ling, Peter Böhlen

[6] Electrophysiological assays of mammalian cells involved in neurohormonal communication Original Research Article
Pages 93-111
Bernard Dufy, Luce Dufy-Barbe, Jeffery L. Barker

[7] Electrophysiological techniques in dissociated tissue culture Original Research Article
Pages 111-132
Gary L. Westbrook, Phillip G. Nelson

[8] Neuroendocrine cells in Vitro: Electrophysiology, triple-labeling with dye marking, immunocytochemical and ultrastructural analysis, and hormone release Original Research Article
Pages 132-147
James N. Hayward, Troy A. Reaves Jr., Robert S. Greenwood, Rick B. Meeker

[9] Single-channel electrophysiology: Use of the patch clamp Original Research Article
Pages 147-176
Frederick Sachs, Anthony Auerbach

[10] Push—pull perfusion technique in the median eminence: A model system for evaluating releasing factor dynamics Original Research Article
Pages 176-187
Norman W. Kasting, Joseph B. Martin

[11] Horseradish peroxidase: A tool for study of the neuroendocrine cell and other peptide-secreting cells Original Research Article
Pages 187-218
Richard D Broadwell, Milton W Brightman

[12] Visualization of enkephalin receptors by image-intensified fluorescence microscopy Original Research Article
Pages 219-227
Steven G. Blanchard, Kwen-Jen Chang, Pedro Cuatrecasas

[13] Flow cytometry as an analytic and preparative tool for studies of neuroendocrine function Original Research Article
Pages 227-245
John C. Cambier, John G. Monroe

[14] Optimization of culture conditions for short-term pituitary cell culture Original Research Article
Pages 249-257
Nira Ben-Jonathan, Edna Peleg, Michael T. Hoefer

[15] Separation of cells from the rat anterior pituitary gland Original Research Article
Pages 257-287
Wesley C. Hymer, J.Michael Hatfield

[16] Culture of dispersed anterior pituitary cells on extracellular matrix Original Research Article
Pages 287-293
Richard I. Weiner, Cynthia L. Bethea, Philippe Jaquet, John S. Ramsdell, Denis J. Gospodarowicz

[17] Continuous perifusion of dispersed anterior pituitary cells: Technical aspects Original Research Article
Pages 294-305
William S. Evans, Michael J. Cronin, Michael O. Thorner

[18] Preparation and maintenance of adrenal medullary chromaffin cell cultures Original Research Article
Pages 305-312
Steven P. Wilson, Norman Kirshner

[19] Techniques for culture of hypothalamic neurons Original Research Article
Pages 313-334
Catherine Loudes, Annie Faivre-Bauman, AndrГ©e Tixier-Vidal

[20] Techniques in the tissue culture of rat sympathetic neurons Original Research Article
Pages 334-347
Mary I. Johnson, Vincent Argiro

[21] Methodological considerations in culturing peptidergic neurons Original Research Article
Pages 347-362
William J. Shoemaker, Robert A. Peterfreund, Wylie Vale

[22] Preparation and properties of dispersed rat retinal cells Original Research Article
Pages 362-368
James M. Schaeffer

[23] Punch sampling biopsy technique Original Research Article
Pages 368-376
MiklГіs Palkovits

[24] Excitotoxic amino acids as neuroendocrine research tools Original Research Article
Pages 379-393
John W. Olney, Madelon T. Price

[25] Use of excitatory amino acids to make axon-sparing lesions of hypothalamus Original Research Article
Pages 393-400
J.Victor Nadler, Debra A. Evenson

[26] Use of specific ion channel activating and inhibiting drugs in neuroendocrine tissue Original Research Article
Pages 401-405
P.Michael Conn

[27] Development and use of ultrasensitive enzyme immunoassays Original Research Article
Pages 409-434
Glennwood E. Trivers, Curtis C. Harris, Catherine Rougeot, Fernand Dray

[28] Strategies for the preparation of haptens for conjugation and substrates for iodination for use in radioimmunoassay of small oligopeptides Original Research Article
Pages 435-447
William W. Youngblood, John Stephen Kizer

[29] Preparation and use of specific antibodies for immunohistochemistry of neuropeptides Original Research Article
Pages 448-459
Lothar Jennes, Walter E. Stumpf

[30] Production of monoclonal antibodies reacting with the cytoplasm and surface of differentiated cells Original Research Article
Pages 459-469
Richard M. Scearce, George S. Eisenbarth

[31] Voltammetric and radioisotopic measurement of catecholamines Original Research Article
Pages 469-483
Paul M. Plotsky

[32] Microradioenzymic assays for the measurement of catecholamines and serotonin Original Research Article
Pages 483-493
Ralph L. Cooper, Richard F. Walker

[33] Hypothalamic catecholamine biosynthesis and neuropeptides Original Research Article
Pages 493-511
David K. Sundberg, Barbara A. Bennett, Mariana Morris

[34] Methods for the study of the biosynthesis of neuroendocrine peptides in vivo and in vitro Original Research Article
Pages 511-523
Jeffrey F. McKelvy, James E. Krause, Jeffrey D. White

[35] Methods for investigating peptide precursors in the hypothalamus Original Research Article
Pages 524-539
David K. Sundberg, Mariana Morris, Kenneth A. Gruber

[36] Measurement of the degradation of luteinizing hormone releasing hormone by hypothalamic tissue Original Research Article
Pages 539-547
James E. Krause, Jeffrey F. McKelvy

[37] Measurement of ОІ-endorphin and enkephalins in biological tissues and fluids Original Research Article
Pages 547-564
Jau-Shyong Hong, Kazuaki Yoshikawa, R.Wayne Hendren

[38] Assay of corticotropin releasing factor Original Research Article
Pages 565-577
Wylie Vale, Joan Vaughan, Gayle Yamamoto, Thomas Bruhn, Carolyn Douglas, David Dalton, Catherine Rivier, Jean Rivier

[39] Direct radioligand measurement of dopamine receptors in the anterior pituitary gland Original Research Article
Pages 577-590
Marc G. Caron, Brian F. Kilpatrick, David R. Sibley

[40] Ornithine decarboxylase: Marker of neuroendocrine and neurotransmitter actions Original Research Article
Pages 590-603
Theodore A. Slotkin, Jorge Bartolome

[41] Secretion of hypothalamic dopamine into the hypophysial portal vasculature: An overview Original Research Article
Pages 607-618
John C. Porter, Marianne J. Reymond, Jun Arita, Janice F. Sissom

[42] Neurotransmitter histochemistry: Comparison of fluorescence and immunohistochemical methods Original Research Article
Pages 619-630
Robert Y. Moore, J.Patrick Card

[43] Simultaneous localization of steroid hormones and neuropeptides in the brain by combined autoradiography and immunocytochemistry Original Research Article
Pages 631-638
Madhabananda Sar, Walter E. Stumpf

[44] Immunocytochemistry of steroid hormone receiving cells in the central nervous system Original Research Article
Pages 639-662
Joan I. Morrell, Donald W. Pfaff

[45] Techniques for tracing peptide-specific pathways Original Research Article
Pages 663-669
Larry W. Swanson

[46] Immunocytochemistry of endorphins and enkephalins Original Research Article
Pages 670-687
Floyd E. Bloom, Elena L.F. Battenberg

[47] Previously published articles from methods in enzymology related to neuroendocrine peptides
Pages 691-692

Author index
Pages 693-720

Subject index
Pages 721-740