Mitophagy: Methods and Protocols

Front Matter ....Pages i-xi
 Short Overview  (Norihiko Furuya)....Pages 3-8
 Induction of PINK1/Parkin-Mediated Mitophagy  (Shigeto Sato, Norihiko Furuya)....Pages 9-17
 Immunocytochemical Monitoring of PINK1/Parkin-Mediated Mitophagy in Cultured Cells  (Motoki Fujimaki, Shinji Saiki, Yukiko Sasazawa, Kei-Ichi Ishikawa, Yoko Imamichi, Katsuhiko Sumiyoshi et al.)....Pages 19-27
 The Use of Correlative Light-Electron Microscopy (CLEM) to Study PINK1/Parkin-Mediated Mitophagy  (Chieko Kishi-Itakura, Folma Buss)....Pages 29-39
 Observation of Parkin-Mediated Mitophagy in Pancreatic β-Cells  (Atsushi Hoshino, Satoaki Matoba)....Pages 41-46
 Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila  (Tsuyoshi Inoshita, Kahori Shiba-Fukushima, Hongrui Meng, Nobutaka Hattori, Yuzuru Imai)....Pages 47-57
 Assessment of Mitophagy in iPS Cell-Derived Neurons  (Kei-Ichi Ishikawa, Akihiro Yamaguchi, Hideyuki Okano, Wado Akamatsu)....Pages 59-67
 Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting  (Sachiyo Nagumo, Koji Okamoto)....Pages 71-83
 MitoPho8Δ60 Assay as a Tool to Quantitatively Measure Mitophagy Activity  (Zhiyuan Yao, Xu Liu, Daniel J. Klionsky)....Pages 85-93
 Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants  (Kentaro Furukawa, Tomotake Kanki)....Pages 95-104
 Flow Cytometer Monitoring of Bnip3- and Bnip3L/Nix-Dependent Mitophagy  (Matilda Šprung, Ivan Dikic, Ivana Novak)....Pages 105-110
 Exploring MicroRNAs on NIX-Dependent Mitophagy  (Wen Li, Hao Chen, Shupeng Li, Guanghong Lin, Du Feng)....Pages 111-121
 Monitoring of Atg5-Independent Mitophagy  (Satoko Arakawa, Shinya Honda, Satoru Torii, Masatsune Tsujioka, Shigeomi Shimizu)....Pages 125-132
 Monitoring of Paternal Mitochondrial Degradation in Caenorhabditis elegans  (Miyuki Sato, Ken Sato)....Pages 133-140
 Detection of Hypoxia-Induced and Iron Depletion-Induced Mitophagy in Mammalian Cells  (Shun-ichi Yamashita, Tomotake Kanki)....Pages 141-149
 Monitoring Mitophagy During Aging in Caenorhabditis elegans  (Nikolaos Charmpilas, Konstantinos Kounakis, Nektarios Tavernarakis)....Pages 151-160
 Monitoring of Iron Depletion-Induced Mitophagy in Pathogenic Yeast  (Koichi Tanabe, Minoru Nagi)....Pages 161-172
 Dopaminergic Neuron-Specific Autophagy-Deficient Mice  (Shigeto Sato, Nobutaka Hattori)....Pages 173-175
Back Matter ....Pages 177-180