Microtubules: in vivo

Content: 
Series Editors
Page ii

Volume Editors
Page iii

Copyright
Page iv

Contributors
Pages xiii-xvi

Preface
Pages xvii-xviii
Lynne Cassimeris, Phong Tran

Chapter 1 - Determination of Microtubule Dynamic Instability in Living Cells
Pages 1-14
Kathy Kamath, Emin Oroudjev, Mary Ann Jordan

Chapter 2 - Analysis of Microtubule Polymerization Dynamics in Live Cells
Pages 15-33
Sarah Gierke, Praveen Kumar, Torsten Wittmann

Chapter 3 - The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics
Pages 35-52
Claire E. Walczak, Rania S. Rizk, Sidney L. Shaw

Chapter 4 - Kinetochore–Microtubule Dynamics and Attachment Stability
Pages 53-79
Jennifer G. DeLuca

Chapter 5 - Photoactivatable Green Fluorescent Protein-Tubulin
Pages 81-90
U. Serdar Tulu, Nick P. Ferenz, Patricia Wadsworth

Chapter 6 - Microtubule Dynamics at the Cell Cortex Probed by TIRF Microscopy
Pages 91-109
Ilya Grigoriev, Anna Akhmanova

Chapter 7 - Microtubule Dynamics in Dendritic Spines
Pages 111-132
Lukas C. Kapitein, Kah Wai Yau, Casper C. Hoogenraad

Chapter 8 - Protein Micropatterns: A Direct Printing Protocol Using Deep UVs
Pages 133-146
Ammar Azioune, Nicolas Carpi, Qingzong Tseng, Manuel Théry, Matthieu Piel

Chapter 9 - New and Old Reagents for Fluorescent Protein Tagging of Microtubules in Fission Yeast: Experimental and Critical Evaluation
Pages 147-172
Hilary A. Snaith, Andreas Anders, Itaru Samejima, Kenneth E. Sawin

Chapter 10 - Optical Trapping and Laser Ablation of Microtubules in Fission Yeast
Pages 173-183
Nicola Maghelli, Iva M. Tolić -Nørrelykke

Chapter 11 - A Fast Microfluidic Temperature Control Device for Studying Microtubule Dynamics in Fission Yeast
Pages 185-201
Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn, Adeline Mayeux, Phong T. Tran

Chapter 12 - Microtubule-Dependent Spatial Organization of Mitochondria in Fission Yeast
Pages 203-221
Maitreyi Das, Stéphane Chiron, Fulvia Verde

Chapter 13 - Microscopy Methods for the Study of Centriole Biogenesis and Function in Drosophila
Pages 223-242
Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini, Monica Bettencourt-Dias

Chapter 14 - Drosophila S2 Cells as a Model System to Investigate Mitotic Spindle Dynamics, Architecture, and Function
Pages 243-257
Sara Moutinho-Pereira, Irina Matos, Helder Maiato

Chapter 15 - Assessment of Mitotic Spindle Phenotypes in Drosophila S2 Cells
Pages 259-275
Gohta Goshima

Chapter 16 - Analysis of Microtubules in Budding Yeast
Pages 277-306
Alexander Rauch, Elena Nazarova, Jackie Vogel

Chapter 17 - Imaging and Analysis of the Microtubule Cytoskeleton in Giardia
Pages 307-339
Scott C. Dawson, Susan A. House

Chapter 18 - Live Cell-Imaging Techniques for Analyses of Microtubules in Dictyostelium
Pages 341-357
Matthias Samereier, Irene Meyer, Michael P. Koonce, Ralph Gräf

Chapter 19 - Imaging of Mitotic Spindle Dynamics in Caenorhabditis elegans Embryos
Pages 359-372
Mika Toya, Yumi Iida, Asako Sugimoto

Chapter 20 - Microtubule Dynamics in Plant Cells
Pages 373-400
Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder, Clive W. Lloyd

Chapter 21 - Melanophores for Microtubule Dynamics and Motility Assays
Pages 401-414
Kazuho Ikeda, Irina Semenova, Olga Zhapparova, Vladimir Rodionov

Chapter 22 - Imaging Cilia in Zebrafish
Pages 415-435
Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher, Rebecca D. Burdine

Chapter 23 - Modeling Microtubule-Mediated Forces and Centrosome Positioning in Caenorhabditis elegans Embryos
Pages 437-453
Akatsuki Kimura, Shuichi Onami

Chapter 24 - Cryo-Electron Tomography of Cellular Microtubules
Pages 455-473
Roman I. Koning

Chapter 25 - Automated Identification of Microtubules in Cellular Electron Tomography
Pages 475-495
Daniyar Nurgaliev, Timur Gatanov, Daniel J. Needleman

Chapter 26 - Quality Control in Single-Molecule Studies of Kinesins and Microtubule-Associated Proteins
Pages 497-506
Gary J. Brouhard

Subject Index
Pages 507-522

Volumes in Series
Pages 523-530