Microglia: Methods and Protocols

Front Matter ....Pages i-xii
Front Matter ....Pages 1-1
 Microglia: The Neural Cells of Nonneural Origin  (Olga Garaschuk, Alexei Verkhratsky)....Pages 3-11
 Morphology of Microglia Across Contexts of Health and Disease  (Julie C. Savage, Micaël Carrier, Marie-Ève Tremblay)....Pages 13-26
 Physiology of Microglia  (Olga Garaschuk, Alexei Verkhratsky)....Pages 27-40
 Microglia Reactivity: Heterogeneous Pathological Phenotypes  (Hélène Hirbec, François Rassendren, Etienne Audinat)....Pages 41-55
 Microglia in Neurodegenerative Disorders  (Darío Tejera, Michael T. Heneka)....Pages 57-67
 Physiological Implications of Microglia–Synapse Interactions  (Hiroaki Wake, Hiroshi Horiuchi, Daisuke Kato, Andrew J. Moorhouse, Junichi Nabekura)....Pages 69-80
Front Matter ....Pages 81-81
 Ultrastructural Analyses of Microglial Interactions with Synapses  (Marie-Ève Tremblay, Ania K. Majewska)....Pages 83-95
 Visualizing Dark Microglia  (Marie-Kim St-Pierre, Maude Bordeleau, Marie-Ève Tremblay)....Pages 97-110
 Electrophysiological Investigation of Microglia  (Elena Avignone, Giampaolo Milior, Isabelle Arnoux, Etienne Audinat)....Pages 111-125
 Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia  (Annette Masuch, Knut Biber)....Pages 127-147
 Assessment of Parasite–Microglia Interactions In Vitro  (Katherine Figarella, Nestor L. Uzcategui, Michael Duszenko)....Pages 149-161
Front Matter ....Pages 163-163
 In Vivo Visualization of Microglia Using Tomato Lectin  (Bianca Brawek, Maria Olmedillas del Moral, Olga Garaschuk)....Pages 165-175
 Long-Term In Vivo Imaging of Individual Microglial Cells  (Angelos A. Skodras, Jasmin K. Hefendehl, Jonas J. Neher)....Pages 177-189
 Analysis of Signaling Mechanisms Regulating Microglial Process Movement  (Vasiliki Kyrargyri, David Attwell, Renaud Blaise Jolivet, Christian Madry)....Pages 191-205
 Measuring Microglial Turnover in the Adult Brain  (Diego Gomez-Nicola, Gemma L. Fryatt, Katharine E. Askew)....Pages 207-215
 Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor  (Maja Kitic, Peter See, Julia Bruttger, Florent Ginhoux, Ari Waisman)....Pages 217-230
 Single-Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia  (Bianca Brawek, Olga Garaschuk)....Pages 231-241
 Labeling Microglia with Genetically Encoded Calcium Indicators  (Yajie Liang, Olga Garaschuk)....Pages 243-265
 Calcium Imaging of Microglial Network Activity in Stroke  (Petr Tvrdik, Kathryn N. Kearns, Khadijeh A. Sharifi, M. Filip Sluzewski, Scott T. Acton, M. Yashar S. Kalani)....Pages 267-279
 In Vivo Two-Photon Imaging of Microglial Synapse Contacts  (Daisuke Kato, Ako Ikegami, Hiroshi Horiuchi, Andrew J. Moorhouse, Junichi Nabekura, Hiroaki Wake)....Pages 281-286
 In Vivo Phagocytosis Analysis of Amyloid Beta  (Darío Tejera, Michael T. Heneka)....Pages 287-292
 Functional Analysis of Brain-Engrafted Monocytes After Microglia Ablation in Mouse Models  (Nicholas H. Varvel, Richard M. Ransohoff, Jonas J. Neher)....Pages 293-301
Front Matter ....Pages 303-303
 Analysis of the Microglial Sensome  (Suzanne E. Hickman, Joseph El Khoury)....Pages 305-323
 Transcriptomics and Live Imaging to Define Functional Phenotypes of Microglia in Pathological Human Tissue  (Giampaolo Milior, Farah Chali, Tiffany Dos Santos, Juliette Royer, Richard Miles, Mélanie Morin-Brureau)....Pages 325-336
Back Matter ....Pages 337-340