Metalloproteins: Methods and Protocols

Front Matter ....Pages i-x
Front Matter ....Pages 1-1
 Nitrogenases  (Nathaniel S. Sickerman, Yilin Hu, Markus W. Ribbe)....Pages 3-24
 Enzymatic Systems with Homology to Nitrogenase: Biosynthesis of Bacteriochlorophyll and Coenzyme F430  (Jürgen Moser, Gunhild Layer)....Pages 25-35
 Carbon Monoxide Dehydrogenases  (Jae-Hun Jeoung, Berta M. Martins, Holger Dobbek)....Pages 37-54
 Molybdenum-Containing Enzymes  (Dimitri Niks, Russ Hille)....Pages 55-63
 Hydrogenases  (Nathaniel S. Sickerman, Yilin Hu)....Pages 65-88
Front Matter ....Pages 89-89
 Genomic Manipulations of the Diazotroph Azotobacter vinelandii  (Patricia C. Dos Santos)....Pages 91-109
 Purification of Nitrogenase Proteins  (Chi-Chung Lee, Markus W. Ribbe, Yilin Hu)....Pages 111-124
 Expression, Purification, and Activity Analysis of Chlorophyllide Oxidoreductase and Ni2+-Sirohydrochlorin a,c-Diamide Reductase  (Jürgen Moser, Jan Jasper, José Vazquez Ramos, Sven T. Sowa, Gunhild Layer)....Pages 125-140
 Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactors  (Paul Kaufmann, Chantal Iobbi-Nivol, Silke Leimkühler)....Pages 141-152
Front Matter ....Pages 153-153
 Crystallization of Nitrogenase Proteins  (Belinda B. Wenke, Renee J. Arias, Thomas Spatzal)....Pages 155-165
 X-Ray Crystallography of Carbon Monoxide Dehydrogenases  (Jae-Hun Jeoung, Berta M. Martins, Holger Dobbek)....Pages 167-178
 X-Ray Absorption Spectroscopy of Metalloproteins  (Limei Zhang)....Pages 179-195
 Electron Paramagnetic Resonance Spectroscopy of Metalloproteins  (Andrew Jasniewski, Yilin Hu, Markus W. Ribbe)....Pages 197-211
 Magnetic Circular Dichroism Spectroscopy of Metalloproteins  (Brian J. Hales)....Pages 213-225
Front Matter ....Pages 227-227
 Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site  (Kazuki Tanifuji, Yasuhiro Ohki)....Pages 229-244
 Computational Methods for Modeling Metalloproteins  (Martin T. Stiebritz, Yilin Hu)....Pages 245-266
Back Matter ....Pages 267-275