Mechanisms of Work Production and Work Absorption in Muscle

Front Matter....Pages i-xv
Introductory Remarks....Pages 1-4
Linear and Rotary Molecular Motors....Pages 5-14
Measurement of ATPase Activities of Myosin at the Level of Tracks and Single Molecules....Pages 15-27
Evanescent Excitation Microscopy....Pages 29-36
Three-Dimensional Image Analysis of Myosin Head in Function as Captured by Quick-Freeze Deep-Etch Replica Electron Microscopy....Pages 37-45
Fluctuation Correlation in the Sliding Movement Generated by Protein Motors In Vitro....Pages 47-51
ATP-Induced Axial Movement of Myosin Heads in Living Thick Filaments Recorded with a Gas Environmental Chamber attached to the Electron Microscope....Pages 53-62
Design Principles and Applications of a Cooled CCD Camera for Electron Microscopy....Pages 63-72
Structure of the Monomeric Actin-Myosin Head Complex as Revealed by X-Ray Solution Scattering....Pages 73-78
The Use of Fast Kinetic Systems for the Study of ng-μg Quantities of Purified Muscle Proteins....Pages 79-89
Inhibitory Actions of Synthetic Actin Peptides and 2,3-Butanedion Monoxime on Actomyosin System....Pages 91-98
Smooth Muscle Myosin....Pages 99-104
The Molecular Biology and Pathophysiology of Hypertrophic Cardiomyopathy Due to Mutations in the Beta Myosin Heavy Chains and the Essential and Regulatory Light Chains....Pages 105-115
Cross-Bridge Dynamics in the Contracting Heart....Pages 117-124
Distinct Kinetic Properties of Cardiac Myosin Isoforms Revealed by In Vitro Studies....Pages 125-130
Functional Characterization of Dictyostelium Discoideum Mutant Myosins Equivalent to Human Familial Hypertrophic Cardiomyopathy....Pages 131-137
Hydrodynamic Properties of Water in Contractile System....Pages 139-144
 1 H-NMR Spectroscopy of the Intracellular Water of Resting and Rigor Frog Skeletal Muscle....Pages 145-155
The Crystal Structure of Troponin C in Complex with N-Terminal Fragment of Troponin I....Pages 157-167
Molecular Switches in Troponin....Pages 169-176
Fluorescence of NBD-Labelled Troponin-I as a Probe for the Kinetics of Thin Filament Activation in Skeletal Muscle Fibers....Pages 177-185
Skeletal Muscle Regulatory Proteins Enhance F-Actin In Vitro Motility....Pages 187-197
Signal Transmission and Transduction in Excitation-Contraction Coupling....Pages 199-207
Regulation of Contraction in Heart Muscle....Pages 209-218
Role of Calcium and Crossbridges in Modulation of Rates of Force Development and Relaxation in Skinned Muscle Fibers....Pages 219-228
Structure and Function of Smooth Muscle Myosin Light Chain Kinase....Pages 229-234
Regulation of Scallop Myosin by Calcium....Pages 235-240
Modulatory Effect of MgATP on Smooth Muscle Myosin Phosphatase Activity....Pages 241-246
Studies on the 14.5 nm Meridional X-Ray Diffraction Reflection During Length Changes of Intact Frog Muscle Fibres....Pages 247-258
On the Working Stroke Elicited by Steps in Length and Temperature....Pages 259-264
Myosin Head Movements during Isometric Contraction Studied by X-Ray Diffraction of Single Frog Muscle Fibres....Pages 265-270
X-Ray Diffraction Studies of the Cross-Bridge Intermediate States....Pages 271-279
Spacing Changes in the Actin and Myosin Filaments during Activation, and Their Implications....Pages 281-288
Structural Features of Force-Generating Cross-Bridges....Pages 289-296
Myosin Crossbridge Configurations in Equilibrium States of Vertebrate Skeletal Muscle....Pages 297-308
Extensibility of the Actin and Myosin Filaments in Various States of Skeletal Muscle as Studied by X-Ray Diffraction....Pages 309-317
Contractile Properties of Thin (Actin) Filament-Reconstituted Muscle Fibers....Pages 319-329
The ATPase Activity in Isometric and Shortening Skeletal Muscle Fibres....Pages 331-341
Force Generation Simplified....Pages 343-352
Evidence for Two Distinct Cross-Bridge Populations in Tetanized Frog Muscle Fibers Stretched with Moderate Velocities....Pages 353-359
Implications of Quantal Motor Action in Biological Systems....Pages 361-371
Calcium Dependence of the Apparent Rate of Force Generation in Single Striated Muscle Myofibrils Activated by Rapid Solution Changes....Pages 373-382
Myofilament Compliance and Sarcomere Tension-Stiffness Relation during the Tetanus Rise in Frog Muscle Fibres....Pages 383-392
Muscle Work Enhancement by Stretch....Pages 393-409
The Physiological Evaluation of Gene Therapies of Dystrophin-Deficient Muscles....Pages 411-417
Communications Between the Nuclotide-and Actin-Binding Site of the Myosin Head in Muscle Fibers....Pages 419-423
Crossbridge Head Detachment Rate Constants Determined from a Model that Explains the Behavior of Both Weakly-and Strongly-Binding Crossbridges....Pages 425-434
Bidirectional Functional Communication between Myosin Subfragments 1 and 2 in Skeletal Muscle Fibers....Pages 435-440
Reappraisal of the Multicellular Preparation for the In Vitro Physiopharmacological Evaluation of Myocardial Performance....Pages 441-451
Alterations of Myocardial Contraction Associated with a Structural Heart Defect in Embryonic Chicks....Pages 453-459
Influences of Protein Kinase A and D-Camp on Actin-Myosin Interaction and Energy Consumption of Cardiac Muscles....Pages 461-470
Work Production and Work Absorption in Muscle Strips from Vertebrate Cardiac and Insect Flight Muscle Fibers....Pages 471-480
Expression and Function of Atrial Myosin Light Chain1 in the Porcine Right Ventricle of Normal and Pulmonary Hypertensive animals....Pages 481-489
Ventricular Pressure-Volume Area (PVA) Accounts for Cardiac Energy Consumption of Work Production and Absorption....Pages 491-498
Oxygen Consumption and Motility of Mechanically Unloaded Myocardial Slices....Pages 499-506
Vascular Endothelial Cell-Cardiac Myocyte Crosstalk in Achieving a Balance between Energy Supply and Energy use....Pages 507-514
Ramp-Rate Dependent External Work During Ramp-Load Release in Cardiac Muscle....Pages 515-526
Efficiency of Skeletal and Cardiac Muscle....Pages 527-536
Muscle Energetics during Unfused Tetanic Contractions....Pages 537-544
Shortening during Stimulation vs. during Relaxation....Pages 545-555
Load-Dependent Mechanical Efficiency of Individual Myosin Heads in Skeletal Muscle Fibers Activated by Laser Flash Photolysis of Caged Calcium in the Presence of a Limited amount of ATP....Pages 557-567
Measurement of ATP Turnover during Shortening and Lengthening of Rabbit Psoas Myofibrils Using a Fluorescent ATP analog....Pages 569-646
Concluding Remarks....Pages 647-653
Back Matter....Pages 655-663