Hybridoma Technology in the Biosciences and Medicine

Front Matter....Pages i-xxv
Front Matter....Pages 1-1
Monoclonal Antibody Strategy and Techniques....Pages 3-41
Strategies for Production of Human Monoclonal Antibodies....Pages 43-55
The Antibody Combining Site....Pages 57-76
The Generation of Better Monoclonal Antibodies through Somatic Mutation....Pages 77-88
Switching the Isotype of Monoclonal Antibodies....Pages 89-101
The Analysis of Structural Diversity in the Antibody Response....Pages 103-115
Expression of Cloned Immunoglobulin Genes....Pages 117-134
Front Matter....Pages 135-135
The Immunochemistry of Monoclonal Antibodies: Studies on One Immunologic Supergene (HLA) with the Products of Another....Pages 137-161
Purification of Membrane Glycoproteins with Monoclonal Antibody Affinity Columns....Pages 163-175
Transferrin Receptor....Pages 177-189
Functional and Structural Interrelationships among the Mac-1, LFA-1 Family of Leukocyte Adhesion Glycoproteins, and their Deficiency in a Novel Heritable Disease....Pages 191-206
Demonstration of Receptor Function of Membrane Proteins by Selection and Immobilization with Monoclonal Antibodies....Pages 207-221
Production of Rat × Mouse Hybridomas for the Study of the Nicotinic Acetylcholine Receptor....Pages 223-228
Sperm Surface Domains....Pages 239-250
The Cytoskeleton....Pages 251-268
Monoclonal Antibodies as Molecular Probes of the Nervous System....Pages 269-289
Application of Monoclonal Antibodies to Issues of Plasma Lipoprotein Structure and Function....Pages 291-308
Enzyme Active Sites....Pages 309-325
Studies of the Hong Kong Influenza Hemagglutinin....Pages 327-340
Virus Receptors on Somatic and Immune Cells....Pages 341-351
Front Matter....Pages 353-353
Designing and Using Site-Specific Antibodies to Synthetic Peptides....Pages 355-373
Gene Transfer as a General Approach to Facilitate the Recombinant DNA Cloning of Surface Antigen Genes....Pages 375-381
Amplification and Molecular Cloning of Transfected Genes....Pages 383-395
Screening λgt11 Expression Libraries with Antibody Probes....Pages 397-406
Purification of Low-Abundance Messenger RNAs by Polysome Isolation with Polyclonal Antibodies....Pages 407-417
Purification of Messenger RNA by Polysome Isolation with Monoclonal Antibodies....Pages 419-426
Expression of Foreign Proteins on the Surface of Xenopus laevis Oocytes....Pages 427-438
Front Matter....Pages 439-439
Antibodies in Vivo ....Pages 441-452
Human Complement-Fixing Antibodies in Transplantation....Pages 453-471
Manipulation of T-Cell Populations to Abrogate Allograft Rejection....Pages 473-492
Serotherapy and Bone Marrow Transplantation....Pages 493-504
Front Matter....Pages 505-505
The Murine T-Cell Antigen: Ia Receptor: Functional and Structural Analysis with a Monoclonal Antireceptor Antibody....Pages 507-526
Functional T-Cell Hybridomas....Pages 527-544
The Mechanisms That Control the Proliferation and Activity of Cytolytic T Lymphocytes....Pages 545-557
Human Cytolytic T-Lymphocyte Clones and Their Function-Associated Cell Surface Molecules....Pages 559-573
The Use of Mouse Lymphocyte Clones to Define T-Cell Gene Products and Analyze Lymphocyte Gene Activation....Pages 575-586
Interleukin 1, Interferon Gamma, and the Modulation of Macrophage-T-Cell Interactions....Pages 587-596
Back Matter....Pages 597-602