Guide to Protein Purification, Second Edition

Content: 
Series Editors
Pages i-iii

Copyright Page
Page iv

Contents
Pages v-xvii

Contributors to Volume 463
Pages xix-xxiv

Preface
Pages xxv-xxvi
Richard R. Burgess, Murray P. Deutscher

Volume in Series
Pages xxvii-liii

Preface to Chapter 1
Page liv
Murray P. Deutscher

Chapter 1 Why Purify Enzymes?
Pages 3-6
Arthur Kornberg

Chapter 2 Strategies and Considerations for Protein Purifications
Pages 9-19
Stuart Linn

Chapter 3 Use of Bioinformatics in Planning a Protein Purification
Pages 21-28
Richard R. Burgess

Chapter 4 Preparing a Purification Summary Table
Pages 29-34
Richard R. Burgess

Chapter 5 Setting Up a Laboratory
Pages 37-42
Murray P. Deutscher

Chapter 6 Buffers: Principles and Practice
Pages 43-56
Vincent S. Stoll, John S. Blanchard

Chapter 7 Measurement of Enzyme Activity
Pages 57-71
T.K. Harris, M.M. Keshwani

Chapter 8 Quantitation of Protein
Pages 73-95
James E. Noble, Marc J.A. Bailey

Chapter 9 Concentration of Proteins and Removal of Solutes
Pages 97-120
David R.H. Evans, Jonathan K. Romero, Matthew Westoby

Chapter 10 Maintaining Protein Stability
Pages 121-127
Murray P. Deutscher

Chapter 11 Selecting an Appropriate Method for Expressing a Recombinant Protein
Pages 131-147
William H. Brondyk

Chapter 12 Bacterial Systems for Production of Heterologous Proteins
Pages 149-168
Sarah Zerbs, Ashley M. Frank, Frank R. Collart

Chapter 13 Expression in the Yeast Pichia pastoris
Pages 169-189
James M. Cregg, Ilya Tolstorukov, Anasua Kusari, Jay Sunga, Knut Madden, Thomas Chappell

Chapter 14 Baculovirus–Insect Cell Expression Systems
Pages 191-222
Donald L. Jarvis

Chapter 15 Recombinant Protein Production by Transient Gene Transfer into Mammalian Cells
Pages 223-238
Sabine Geisse, Cornelia Fux

Chapter 16 Tagging for Protein Expression
Pages 239-258
Arun Malhotra

Chapter 17 Refolding Solubilized Inclusion Body Proteins
Pages 259-282
Richard R. Burgess

Chapter 18 Advances in Preparation of Biological Extracts for Protein Purification
Pages 285-303
Anthony C. Grabski

Chapter 19 Isolation of Subcellular Organelles and Structures
Pages 305-328
Uwe Michelsen, Jörg von Hagen

Chapter 20 Protein Precipitation Techniques
Pages 331-342
Richard R. Burgess

Chapter 21 Affi-Gel Blue for Nucleic Acid Removal and Early Enrichment of Nucleotide Binding Proteins
Pages 343-345
Murray P. Deutscher

Chapter 22 Ion-Exchange Chromatography
Pages 349-371
Alois Jungbauer, Rainer Hahn

Chapter 23 Gel Filtration
Pages 373-385
Earle Stellwagen

Chapter 24 Protein Chromatography on Hydroxyapatite Columns
Pages 387-404
Larry J. Cummings, Mark A. Snyder, Kimberly Brisack

Chapter 25 Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification Applications
Pages 405-414
Justin T. McCue

Chapter 26 Affinity Chromatography: General Methods
Pages 417-438
Marjeta Urh, Dan Simpson, Kate Zhao

Chapter 27 Immobilized-Metal Affinity Chromatography (IMAC): A Review
Pages 439-473
Helena Block, Barbara Maertens, Anne Spriestersbach, Nicole Brinker, Jan Kubicek, Roland Fabis, Jörg Labahn, Frank Schäfer

Chapter 28 Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for Immunoaffinity Chromatography
Pages 475-494
Nancy E. Thompson, Katherine M. Foley, Elizabeth S. Stalder, Richard R. Burgess

Chapter 29 One-Dimensional Gel Electrophoresis
Pages 497-513
David E. Garfin

Chapter 30 Isoelectric Focusing and Two-Dimensional Gel Electrophoresis
Pages 515-540
David B. Friedman, Sjouke Hoving, Reiner Westermeier

Chapter 31 Protein Gel Staining Methods: An Introduction and Overview
Pages 541-563
Thomas H. Steinberg

Chapter 32 Elution of Proteins from Gels
Pages 565-572
Richard R. Burgess

Chapter 33 Performing and Optimizing Western Blots with an Emphasis on Chemiluminescent Detection
Pages 573-599
Alice Alegria-Schaffer, Andrew Lodge, Krishna Vattem

Chapter 34 Detergents: An Overview
Pages 603-617
Dirk Linke

Chapter 35 Purification of Membrane Proteins
Pages 619-629
Sue-Hwa Lin, Guido Guidotti

Chapter 36 Purification of Recombinant G-Protein-Coupled Receptors
Pages 631-645
Reinhard Grisshammer

Chapter 37 Cell-Free Translation of Integral Membrane Proteins into Unilamelar Liposomes
Pages 647-673
Michael A. Goren, Akira Nozawa, Shin-ichi Makino, Russell L. Wrobel, Brian G. Fox

Chapter 38 Determination of Protein Purity
Pages 677-689
David G. Rhodes, Thomas M. Laue

Chapter 39 Determination of Size, Molecular Weight, and Presence of Subunits
Pages 691-723
David G. Rhodes, Robert E. Bossio, Thomas M. Laue

Chapter 40 Identification and Quantification of Protein Posttranslational Modifications
Pages 725-763
Adam R. Farley, Andrew J. Link

Chapter 41 Parallel Methods for Expression and Purification
Pages 767-785
Scott A. Lesley

Chapter 42 Techniques to Isolate O2-Sensitive Proteins: [4Fe–4S]-FNR as an Example
Pages 787-805
Aixin Yan, Patricia J. Kiley

Chapter 43 Rethinking Your Purification Procedure
Pages 809-811
Murray P. Deutscher

Chapter 44 Important but Little Known (or Forgotten) Artifacts in Protein Biochemistry
Pages 813-820
Richard R. Burgess

Author Index
Pages 821-833

Subject Index
Pages 835-851