Dictyostelium discoideum: Molecular Approaches to Cell Biology

Content: 
Series Editor
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Edited by
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Copyright Page
Page iv

Preface
Pages xv-xvi
James A. Spudich

Part 1. General Principles
Page 1

Chapter 1 Introductory Remarks and Some Biochemical Considerations Original Research Article
Pages 3-8
James A. Spudich

Chapter 2 Cultivation and Synchronous Morphogenesis of Dictyostelium under Controlled Experimental Conditions Original Research Article
Pages 9-29
Maurice Sussman

Chapter 3 Genetic Tools for Dictyostelium discoideum Original Research Article
Pages 31-65
William F. Loomis

Chapter 4 Molecular Biology in Dictyostelium: Tools and Applications Original Research Article
Pages 67-100
W. Nellen, S. Datta, C. Reymond, A. Sivertsen, S. Mann, T. Crowley, R.A. Firtel

Part II. Vesicular Traffic in Cells
Page 101

Chapter 5 Purification and Characterization of Dictyostelium discoideum Plasma Membranes Original Research Article
Pages 103-128
Catherine M. Goodloe-Holland, Elizabeth J. Luna

Chapter 6 Endocytosis and Recognition Mechanisms in Dictyostelium discoideum Original Research Article
Pages 129-137
Günter Vogel

Chapter 7 Defining the Intracellular Localization Pathways followed by Lysosomal Enzymes in Dictyostelium discoideum Original Research Article
Pages 139-155
James A. Cardelli, George S. Golumbeski, Nancy A. Woychik, David L. Ebert, Robert C. Mierendorf, Randall L. Dimond

Chapter 8 The Axenic Mutations and Endocytosis in Dictyostelium Original Research Article
Pages 157-176
Margaret Clarke, Samuel C. Kayman

Part III. Cell Motility
Page 177

Chapter 9 Introduction of Exogenous Molecules into the Cytoplasm of Dictyostelium discoideum Amoebae by Controlled Sonication Original Research Article
Pages 179-190
Marcus Fechheimer, D.L. Taylor

Chapter 10 Ultrastructural Localization of Cytoskeletal Proteins in Dictyostelium Amoebae Original Research Article
Pages 191-207
J. Condeelis, S. Ogihara, H. Bennett, J. Carboni, A. Hall

Chapter 11 Isolation of the Actin Cytoskeleton from Amoeboid Cells of Dictyostelium Original Research Article
Pages 209-214
Annamma Spudich

Chapter 12 Preparation of Deuterated Actin from Dictyostelium discoideum Original Research Article
Pages 215-229
Deborah B. Stone, Paul M.G. Curmi, Robert A. Mendelson

Chapter 13 Amino-Terminal Processing of Dictyostelium discoideum Actin Original Research Article
Pages 231-243
Peter A. Rubenstein, K.L. Redman, L.R. Solomon, D.J. Martin

Chapter 14 Biochemical and Genetic Approaches to Microtubule Function in Dictyostelium discoideum Original Research Article
Pages 245-259
Eileen White, Eugene R. Katz

Chapter 15 Probing the Mechanisms of Mitosis with Dictyostelium discoideum Original Research Article
Pages 261-279
Urs-Peter Roos

Part IV. Cbemotaxis
Page 281

Chapter 16 Measurement of Chemotaxis in Dictyostelium Original Research Article
Pages 283-298
Theo M. Konijn, Peter J. M. Van Haastert

Chapter 17 Transmembrane Signaling in Dictyostelium Original Research Article
Pages 299-331
Peter Devreotes, Donna Fontana, Peter Klein, Jane Sherring, Anne Theibert

Chapter 18 Identification of Chemoattractant-Elicited Increases in Protein Phosphorylation Original Research Article
Pages 333-345
Catherine Berlot

Chapter 19 Agar-Overlay Immunofluorescence: High-Resolution Studies of Cytoskeletal Components and Their Changes during Chemotaxis Original Research Article
Pages 347-356
Yoshio Fukui, Shigehiko Yumura, Toshiko K. Yumura

Part V. Cell Adhesion and Cell–Cell Recognition
Page 357

Chapter 20 Cell Adhesion: Its Quantification, Assay of the Molecules Involved, and Selection of Defective Mutants in Dictyostelium and Polysphondylium Original Research Article
Pages 359-385
Salvatore Bozzaro, Rainer Merkl, GÜNther Gerisch

Chapter 21 Discoidins I and II: Endogenous Lectins Involved in Cell—Substratum Adhesion and Spore Coat Formation Original Research Article
Pages 387-409
S.H. Barondes, D.N.W. Cooper, W.R. Springer

Part VI. Development and Pattern Formation
Pages 411-412

Chapter 22 Methods for Manipulating and Investigating Developmental Timing in Dictyostelium discoideum Original Research Article
Pages 413-431
David R. Soll

Chapter 23 Cell Differentiation in Monolayers and the Investigation of Slime Mold Morphogens Original Research Article
Pages 433-448
Robert R. Kay

Chapter 24 Vital Staining Methods Used in the Analysis of Cell Sorting in Dictyostelium discoideum Original Research Article
Pages 449-459
Cornelis J. Weijer, Charles N. David, John Sternfeld

Chapter 25 Isolation of Developmentally Regulated Genes Original Research Article
Pages 461-470
Rex L. Chisholm

Chapter 26 A Strategy to Study Development and Pattern Formation: Use of Antibodies against Products of Cloned Genes Original Research Article
Pages 471-487
Richard H. Gomer

Chapter 27 Homologous Recombination in Dictyostelium as a Tool for the Study of Developmental Genes Original Research Article
Pages 489-495
Arturo De Lozanne

Appendix Codon Freqzlency in Dictyostelizcm
Pages 497-500
Hans M. Warrick

Index
Pages 501-516