Antibody Production in Man: In Vitro Synthesis and Clinical Implications

Front Cover......Page 1
Antibody Production in Man: In Vitro Synthesis and Clinical Implications......Page 4
Copyright Page......Page 5
Table of Contents......Page 6
CONTRIBUTORS......Page 10
PREFACE......Page 14
CHAPTER 1. CONSIDERATION OF ANTIBODY DEFICIENCY DISEASES IN RELATION TO NORMAL B-CELL DEVELOPMENT......Page 16
I. NORMAL DEVELOPMENT OF B CELLS......Page 17
REFERENCES......Page 28
I. INTRODUCTION......Page 32
II. MATERIALS AND METHODS......Page 33
III. RESULTS
......Page 37
IV. DISCUSSION......Page 43
REFERENCES......Page 47
I. INTRODUCTION......Page 50
II. OPTIMAL CONDITIONS FOR THE INDUCTION OF POLYCLONAL ANTIBODY RESPONSE BY LPS......Page 52
III. LOCALIZATION OF B CELLS RESPONDING TO DIFFERENT BACTERIAL STRAINS WITH ANTIBODY FORMATION......Page 55
IV. PBA PROPERTIES OF DIFFERENT BACTERIAL PREPARATIONS......Page 57
V. AGE-DEPENDENT RESPONSE TO DIFFERENT BACTERIA IN ADENOID LYMPHOCYTES......Page 58
VI. POLYCLONAL B CELL RESPONSE TO PPD IN CHILDREN WITH BCG INFECTION......Page 59
VII. SUMMARY......Page 61
REFERENCES......Page 62
I. INTRODUCTION......Page 64
II. METHODS......Page 65
III. RESULTS......Page 66
IV. DISCUSSION......Page 73
REFERENCES......Page 75
PART I: GENERAL DISCUSSION MITOGEN INDUCED B CELL ACTIVATION. I.......Page 76
I. INTRODUCTION......Page 84
II. MATERIALS AND METHODS......Page 85
III. RESULTS
......Page 89
IV. DISCUSSION......Page 95
V. SUMMARY......Page 97
REFERENCES......Page 98
I. INTRODUCTION......Page 100
II. MATERIALS AND METHODS......Page 101
Ill. RESULTS......Page 103
IV. DISCUSSION......Page 109
REFERENCES......Page 112
I. INTRODUCTION......Page 116
II. MATERIAL AND METHODS......Page 117
III. RESULTS......Page 120
REFERENCES......Page 127
PART II: GENERAL DISCUSSION MITOGEN-INDUCED B-CELL ACTIVATION. II.......Page 130
I. INTRODUCTION......Page 136
II. MATERIALS AND METHODS......Page 137
III. RESULTS AND DICUSSION......Page 138
REFERENCES......Page 152
I. INTRODUCTION......Page 156
II. MATERIALS AND METHODS......Page 157
III. RESULTS
......Page 161
IV. DISCUSSION......Page 165
V. SUMMARY......Page 170
REFERENCES......Page 171
I. INTRODUCTION......Page 174
III. RESULTS......Page 175
IV. DISCUSSION......Page 178
REFERENCES......Page 180
PART III: GENERAL DISCUSSION ANTIGEN INDUCED B CELL ACTIVATION. I.......Page 182
CHAPTER 11. T - B COLLABORATION IN THE IN VITRO AB RESPONSE TO DIPHTHERIA TOXOID; EVIDENCE FAVOURING A ROLE FOR B CELLS IN THE GENERATION OF T CELL HELP......Page 188
REFERENCES......Page 197
I. INTRODUCTION......Page 200
III. EFFECT OF EPSTEIN-BARR VIRUS (EBV)......Page 201
IV. EFFECT OF MODIFIED CULTURE CONDITIONS......Page 204
REFERENCES......Page 206
CHAPTER 13. HUMAN ANTIGEN SPECIFIC HELPER T CELL FACTORS IN B CELL RESPONSES......Page 208
II. MATERIALS AND METHODS......Page 209
Ill. RESULTS......Page 213
IV. DISCUSSION......Page 217
REFERENCES......Page 220
PART IV: GENERAL DISCUSSION ANTIGEN INDUCED B CELL ACTIVATION. II.......Page 224
I. INTRODUCTION......Page 230
II. MATERIALS AND METHODS......Page 231
III. RESULTS AND DISCUSSION......Page 232
REFERENCES......Page 235
I. INTRODUCTION......Page 238
II. MATERIALS AND METHODS......Page 239
III. RESULTS AND DISCUSSION......Page 240
REFERENCES......Page 245
I. INTRODUCTION......Page 246
II. MATERIALS AND METHODS......Page 247
III. RESULTS......Page 250
IV. DISCUSSION......Page 259
ACKNOWLEDGEMENTS......Page 265
REFERENCES......Page 266
PART V: GENERAL DISCUSSION SUBPOPULATIONS OF LYMPHOCYTES INVOLVED IN THE IN VITRO REGULATION OF B CELL FUNCTION. I.......Page 268
CHAPTER 17. GENERATION OF PLAQUE-FORMING CELLS: ALLOGENEIC EFFECT AND SIGNIFICANCE OF MONOCYTE REMOVAL......Page 274
I. MATERIALS AND METHODS......Page 275
II. RESULTS......Page 277
III. DISCUSSION......Page 282
ACKNOWLEDGMENTS......Page 285
REFERENCES......Page 286
I. INTRODUCTION......Page 288
II. POLYCLONAL INDUCTION OF AN IN VITRO PFC RESPONSE......Page 289
III. CHARACTERIZATION OF HUMAN PERIPHERAL BLOOD SUPPRESSOR CELLS......Page 294
IV. DISCUSSION......Page 301
REFERENCES......Page 303
I. INTRODUCTION......Page 306
II. METHODS......Page 307
III. RESULTS......Page 309
REFERENCES......Page 316
PART VI: GENERAL DISCUSSION SUBPOPULATIONS OF LYMPHOCYTES INVOLVED IN THE IN VITRO REGULATION OF B CELL-FUNCTION. II.......Page 318
I. INTRODUCTION......Page 324
II. PRIMARY HUMORAL IMMUNODEFICIENCY......Page 325
III. SEVERE COMBINED IMMUNODEFICIENCY DISEASE......Page 335
REFERENCES......Page 338
CHAPTER 21. IMMUNOGLOBULIN SECRETING CELLS IN THE CIRCULATING BLOOD AND IN MITOGEN STIMULATED CULTURES FROM PATIENTS WITH IMMUNODEFICIENCY DISEASES......Page 340
REFERENCES......Page 346
CHAPTER 22. POLYCLONAL B-CELL ACTIVATORS IN THE ANALYSIS OF IMMUNODEFICIENCY STATES......Page 348
I. SPECIFIC UNRESPONSIVENESS DUE TO LACK OF V GENE EXPRESSION......Page 349
III. UNRESPONSIVENESS DUE TO LACK OF A PARTICULAR PBA RECEPTOR......Page 351
IV. UNRESPONSIVENESS DUE TO LACK OF A SUBSTANTIAL SUBPOPULATION OF B CELLS......Page 352
V. UNRESPONSIVENESS DUE TO INDUCTION OF IMMUNOLOGICAL TOLERANCE......Page 353
VI. GENERALIZED IMMUNODEFICIENCIES......Page 355
REFERENCES......Page 357
PART VII: GENERAL DISCUSSION CLINICAL IMPLICATIONS IN THE STUDY OF IN VITRO B CELL FUNCTION. I.......Page 360
I. INTRODUCTION......Page 366
II. MATERIAL AND METHODS......Page 367
III. RESULTS......Page 369
REFERENCES......Page 380
CHAPTER 24. THE CELLULAR DEFECT IN IgA DEFICIENCY: POSSIBLE LINKS TO HYPOGAMMAGLOBULINAEMIA......Page 382
REFERENCES......Page 391
I. INTRODUCTION......Page 394
II. MATERIALS AND METHODS......Page 395
III. RESULTS......Page 396
IV. DISCUSSION......Page 400
REFERENCES......Page 401
PART VIII: GENERAL DISCUSSION CLINICAL IMPLICATIONS IN THE STUDY OF IN VITRO B CELL FUNCTION. II.......Page 404
SUMMARY OF WORKSHOP: HUMAN B CELL FUNCTION: RECENT ADVANCES, UNANSWERED QUESTIONS, AND FUTURE DIRECTIONS......Page 408
INDEX......Page 412