Host-Pathogen Interactions: Methods and Protocols

Front Matter ....Pages i-xvi
 Genetic Association Studies in Host–Pathogen Interaction Analysis  (Jose Luis Royo, Luis Miguel Real)....Pages 1-11
 Bacterial Genotyping Methods: From the Basics to Modern  (Margarita M. Ochoa-Díaz, Silvana Daza-Giovannetty, Doris Gómez-Camargo)....Pages 13-20
Front Matter ....Pages 21-21
 Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens  (Gili Aviv, Ohad Gal-Mor)....Pages 23-32
 Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection  (Gili Aviv, Ohad Gal-Mor)....Pages 33-38
 lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens  (Gili Aviv, Ohad Gal-Mor)....Pages 39-45
 Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity  (Gili Aviv, Ohad Gal-Mor)....Pages 47-54
 Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage  (Carlos Medina, Beatriz Mesa-Pereira, Eva M. Camacho, Amando Flores, Eduardo Santero)....Pages 55-70
 Organoids as a Model to Study Infectious Disease  (Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, Eitaro Aihara)....Pages 71-81
 Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins  (Anita Horvatić, Josipa Kuleš, Nicolas Guillemin, Franjo Martinković, Iva Štimac, Vladimir Mrljak et al.)....Pages 83-96
 Systems Biology Modeling to Study Pathogen–Host Interactions  (Müberra Fatma Cesur, Saliha Durmuş)....Pages 97-112
 Phage Therapy: Various Perspectives on How to Improve the Art  (Stephen T. Abedon)....Pages 113-127
Front Matter ....Pages 129-129
 Application of RNA-seq and Bioimaging Methods to Study Microbe–Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression  (Cristina Isabel Amador, Claus Sternberg, Lars Jelsbak)....Pages 131-158
 Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth  (Fernando Govantes)....Pages 159-169
 Detection of Bacterial Quorum Sensing Molecules  (Elke Stein, Adam Schikora)....Pages 171-179
Front Matter ....Pages 181-181
 Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae  (José S. Rufián, Diego López-Márquez, Nieves López-Pagán, Murray Grant, Javier Ruiz-Albert, Carmen R. Beuzón)....Pages 183-199
 Introduction of Genetic Material in Ralstonia solanacearum Through Natural Transformation and Conjugation  (Anthony Perrier, Patrick Barberis, Stéphane Genin)....Pages 201-207
 In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors  (Fabien Lonjon, Nemo Peeters, Stéphane Genin, Fabienne Vailleau)....Pages 209-222
 Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains  (Arry Morel, Nemo Peeters, Fabienne Vailleau, Patrick Barberis, Gaofei Jiang, Richard Berthomé et al.)....Pages 223-239
 Methods to Quantify Biotic-Induced Stress in Plants  (Marcel Bach-Pages, Gail M. Preston)....Pages 241-255
 From Sample to Data: Preparing, Obtaining, and Analyzing Images of Plant-Pathogen Interactions Using Confocal Microscopy  (Helen N. Fones, George R. Littlejohn)....Pages 257-262
 Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria  (Jochen Schmid, Broder Rühmann, Volker Sieber, Lorena Romero-Jiménez, Juan Sanjuán, Daniel Pérez-Mendoza)....Pages 263-275
 Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria  (Marta Robledo, Natalia I. García-Tomsig, José I. Jiménez-Zurdo)....Pages 277-295
 A New, Nondestructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean  (Ángeles Hidalgo, José E. Ruiz-Sainz, José M. Vinardell)....Pages 297-306
 Methods for the Characterization of Plant-Growth Promoting Rhizobacteria  (Md. Motaher Hossain, Farjana Sultana)....Pages 307-328
Back Matter ....Pages 329-332