Working with Stem Cells

Front Matter....Pages i-xviii
Stem Cells: Principles and Applications....Pages 1-13
Human Embryonic Stem Cell Line Derivation....Pages 15-36
Adipose-Derived Mesenchymal Stromal Cells....Pages 37-55
Neural Differentiation of Rodent Neural Progenitor Cells and Isolation and Enrichment of Human Neural Progenitor/Stem Cells....Pages 57-77
Mice Post-natal Subventricular Zone Neurospheres: Derivation, Culture, Differentiation and Applications....Pages 79-96
Very Small Embryonic Like Stem Cells (VSELs) and Their Hematopoietic Specification....Pages 97-110
Neural Crest Stem Cell Cultures: Establishment, Characterization and Potential Use....Pages 111-125
Cancer Stem Cells: Issues with In Vitro Expansion and Model Systems....Pages 127-142
Spontaneous Generation of Patient-Specific Retinal Pigment Epithelial Cells Using Induced Pluripotent Stem Cell Technology....Pages 143-161
Differentiation of Human Pluripotent Stem Cells into Cortical Neurons....Pages 163-180
Motor Neuron Differentiation from Pluripotent Stem Cells: Development of the Technique, Synopsis of Protocols, and Summary of Current Applications....Pages 181-202
Derivation of Dopaminergic Neurons from Human Pluripotent Stem Cells Using a Defined System and/or Small Molecules....Pages 203-217
Differentiation of hiPSC-Derived Cardiomyocytes....Pages 219-236
Endoderm Differentiation from Human Pluripotent Stem Cells....Pages 237-255
Pancreatic Differentiation from Human Pluripotent Stem Cells....Pages 257-275
Efficient Generation of Skeletal Myogenic Progenitors from Human Pluripotent Stem Cells....Pages 277-285
Genome Editing in Stem Cells....Pages 287-309
Neural Stem Cells: Functional Multipotency and Spinal Cord Injury Research Protocols....Pages 311-329
Stem Cells and Tissue Engineering....Pages 331-346
Scaffolds for Embryonic Stem Cell Growth and Differentiation....Pages 347-365
Back Matter....Pages 367-374