Whole-Cell Biocatalysis. Next-Generation Technology for Green Synthesis of Pharmaceutical, Chemicals, and Biofuels

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Half Title
Whole-Cell Biocatalysis: Next-Generation Technology for Green Synthesis of Pharmaceutical, Chemicals, and Biofuels
Copyright
About the Editors
Contents
Contributors
Abbreviations
Acknowledgment
Preface
Part I: Introduction to Whole-Cell Catalysis
	1. Whole‑Cell Biocatalysts: Introduction, Origin, and Concept
		Abstract
		1.1 Introduction
		1.2 Catalysis vs. Biocatalysis
			1.2.1 Traditional Catalysis
			1.2.2 Concept of Biocatalysis
			1.2.3 Origin of WCB
		1.3 Concept of Whole-Cell Biocatalysis
		1.4 Prerequisites for Optimal Chassis Selection
		1.5 Strategies Used for Development of WCB
			1.5.1 Blocking of Competitive Pathways
			1.5.2 Expression of Heterologous Pathways or Enzyme
			1.5.3 Single and Multistep Biocatalysis
			1.5.4 Enhancing the Precursor Supply and Co-Factor Regeneration
		1.6 Tools for Engineering the WCBS
			1.6.1 DNA Elements and Their Use for Controlled Expression
			1.6.2 Genome Editing Using Molecular Tools
			1.6.3 Computational Tools for In-Silico Analysis
		1.7 Conclusions and Future Prospective
		Keywords
		References
	2. Tools and Techniques for the Development of Whole‑Cell Biocatalysis
		Abstract
		2.1 Introduction
		2.2 System Design and Approach for Building a WCB
			2.2.1 Genome Scale Models (GSMS)-Computational Simulator of Cell Metabolism
		2.3 Engineering Inherent Enzymes for Target WCB Process
			2.3.1 Directed Evolution
			2.3.2 Rational Design for Selective Engineering
			2.3.3 Semi-Rational Design
			2.3.4 De Novo Design
		2.4 Tools for Expression of Enzymes/Enzyme Cascade
			2.4.1 Plasmid and Copy Number
			2.4.2 Promoters
			2.4.3 Codon Bias
		2.5 Tools for Gene Editing
			2.5.1 Zinc Finger Nuclease System
			2.5.2 Transcription Activator-Like Effectors (TALEs)
			2.5.3 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)
				2.5.3.1 Mechanism
				2.5.3.2 For Fabrication of Cells
			2.5.4 Chromosomal Integration
		2.6 Tools for Gene Regulation
			2.6.1 Regulatory RNAs
			2.6.2 Riboswitches
			2.6.3 Noncoding Small RNAs
		2.7 Conclusions
		Keywords
		References
Part II: Wcb for Chemicals
	3. Whole-Cell Catalysts: Sustainable Green-Chemical Producing Entities
	4. Microbial Whole-cell Platforms for the Synthesis of Tricarboxylic Acid Cycle-Based Platform Chemicals
	5. System-Enabled Microbial Cell Factories for the Production of Biomolecules
	6. Biotherapeutic Potential and Properties of Seaweeds
	7. Synthesis and Applications of Biopharmaceuticals from Whole-Cell Actinobacterial Isolates
Part III. WCB for Fuels
	8. Microbial Consortia: A Mixed Cell Catalyst for Biotransformation of Biomass into Biofuels and Chemicals
	9. Biofuels: Fueling the Future with Whole-Cell-Derived Fuels
	10. Bioelectrochemical Systems for the Conversion of CO2 into Sustainable Production of Fuels
	11. State-of-the-Art of Microbial Whole-Cell Catalysts for Biofuel Production
	12. Microbial Fuel Cells: Whole-Cell System for MFC, Current Trends, and Future Prospects for the Green Energy
	13. Microalgal Cell: A Machinery for Biofuels Production
	14. Microalgal Cell System for Biofuel Production: Present Leads and Future Prospects
	15. Recent Advances in Algal Bio-Cathode Powered Microbial Fuel Cells
Part IV: Whole-Cell Biocatalysts for Environment Restoration
	16. Production of Biosurfactant by Microbes and Its Application in Biodegradation of Pollutants
	17. Biodegradation of Plastic Wastes by Microbial Cells
	18. Phototrophic Carbon Capture Using Natural Microalgal Whole-Cell Support: An Eco-Technological Approach
	19. Role of Downstream Processing for Production and Purification of Fermentation-Based Products Produced via Whole-Cell Biotransformation
	20. Microbial Cell Systems for Polyhydroxyalkanoate Production and Its Diverse Applications
Index