Understanding light microscopy

Cover......Page 1
Current and future titles in the Royal Microscopical Society – John Wiley Series......Page 3
Understanding Light Microscopy......Page 4
© 2019......Page 5
Dedication......Page 6
Contents......Page 8
About the Author......Page 10
Acknowledgements......Page 12
Look-Up Guide to Feature Boxes by Theme......Page 13
Glossary and Definitions......Page 15
Notes......Page 25
Introduction......Page 28
1 Our Eyes and the Microscope......Page 32
2 Light......Page 60
3 Basic Microscope Optics......Page 86
4 Microscope Anatomy and Design......Page 106
5 Ergonomics......Page 122
6 Optical Aberrations of the Microscope......Page 132
7 The Microscope Objective......Page 158
8 Condensers and Eyepieces......Page 192
9 Illumination in the Microscope......Page 208
10 Diffraction and Image Formation in Microscopy......Page 242
11 Contrast Generation and Enhancement......Page 274
12 Reflected-Light Microscopy......Page 320
13 Polarised-Light Microscopy: Part 1 – Theory......Page 348
14 Polarised-Light Microscopy: Part 2 – Applied......Page 378
15 Fluorescence Microscopy......Page 414
16 Fluorophores and Fluorescent Proteins......Page 436
17 Optical Sectioning and Confocal Microscopy......Page 456
18 Operating the Confocal Microscope......Page 478
19 Light-Sheet Microscopy......Page 514
20 Bleed-Through and Spectral Unmixing......Page 538
21 Deconvolution......Page 554
22 Multi-Photon Microscopy......Page 574
23 Total Internal Reflection Fluorescence Microscopy......Page 592
24 FRAP and FRET......Page 600
25 Colocalisation......Page 618
26 Super-Resolution Fluorescence Microscopy......Page 644
27 Choosing a Microscope Platform andCore Imaging Facilities......Page 668
28 Biological Specimen Preparation......Page 694
29 Materials Specimen Preparation......Page 718
30 Recording the Image: Part 1 – Theory......Page 738
31 Recording the Image: Part 2 – Applied......Page 764
Appendix 1.Buying, and Tendering for, a Light Microscope......Page 800
Appendix 2.Troubleshooting Poor Image Quality......Page 804
Appendix 3.The Michel-L´evy Interference Colour Chart......Page 806
Appendix 4.Cleaning and Maintenance of the Light Microscope......Page 810
Appendix 5. Selected Suppliers......Page 814
Appendix 6.Historical Background......Page 818
Appendix 7.Timeline of Key Events......Page 822
Index......Page 830
Poor Image Quality – fault-finding chart......Page 847