Transgenic Organisms and Biosafety: Horizontal Gene Transfer, Stability of DNA, and Expression of Transgenes

Front Matter....Pages I-XX
Front Matter....Pages 1-1
Gene Transfer Between Organelles and the Nucleus in Lower Eukaryotes....Pages 3-14
Horizontal Transfers and the Evolution of Transposable Elements....Pages 15-30
Transposable Elements in Baculoviruses: Their Possible Role in Horizontal Gene Transfer....Pages 31-41
From Gene Transfer to Risk Assessment: Experiences with Genetic Variability in Baculoviruses and New Approaches to Analyse the Reaction of Microbial Communities to Stress Factors....Pages 43-66
Examination of the Putative Horizontal Gene Transfer from Transgenic Plants to Agrobacteria ....Pages 67-70
Can Agrobacteria Be Eliminated from Transgenic Plants?....Pages 71-76
Preliminary Findings of DNA Transfer from Transgenic Plants to a Wild-Type Strain of Aspergillus niger ....Pages 77-84
Gene Dispersal from Transgenic Crops....Pages 85-93
Evaluation of Potential Gene Transfer from Transgenic Plants....Pages 95-100
Efficiency of Horizontal Transfer by Natural Genetic Transformation of Plasmids with Homology to the Pseudomonas stutzeri Chromosome and the Effect of DNA Restriction....Pages 101-104
Accidental Formation of Replication-Competent Viruses from Gene Transfer by Retroviral Vectors....Pages 105-115
Transgenic Expression of a Toxin-Coding Killer Virus of the Yeast Zygosaccharomyces bailii in Saccharomyces cerevisiae : Genetic Evidence for a Possible Function of “Cryptic” Mycoviruses in the Evolution of Their Hosts....Pages 117-124
Foreign DNA in Mammalian Cells and Organisms....Pages 125-133
Front Matter....Pages 135-135
Persistence of DNA in the Environment and Its Potential for Bacterial Genetic Transformation....Pages 137-146
Persistence of DNA in Natural Soils: Adsorption to Particulate Material Provides Protection Against Nucleolytic Degradation....Pages 147-154
Degradation of Transforming DNA in a Groundwater Aquifer Microcosm by the Extracellular Nuclease of Serratia marcescens SM6 and Application of the Nuclease in a New Conditional Suicide System for Escherichia coli ....Pages 155-163
A Conditional Suicide System for Genetically Engineered Bacteria Based on the Endonuclease I Gene of Escherichia coli ....Pages 165-169
Identification and Characterization of Extrachromosomal Circular DNA Released from a Genetically Modified Chromosome of Hansenula polymorpha ....Pages 171-180
Instability of Tandem Repetitive DNA in “Natural” and Transgenic Organisms....Pages 181-208
Front Matter....Pages 209-209
Range of Expression and Transmission Instability of the Kanamycin-Resistance Reporter Gene in Direct Gene Transfer Experiments....Pages 211-221
Front Matter....Pages 209-209
Variability of Organ-Specific Expression of Reporter Genes in Transgenic Plants....Pages 223-230
Heat-Induced Loss of Transgene Activity Detected and Analysed in Several Transgenic Nicotiana tabacum Lines....Pages 231-238
Quantification of Heat Treatment-Induced Transgene Silencing Using Single Cells of Nicotiana tabacum ....Pages 239-247
Genetic Stability and Expression of Foreign Genes in Transgenic Lines of the Legume Vicia narbonensis ....Pages 249-260
Characterization of a Plant Scaffold-Attached Region from a T-DNA Integration Site....Pages 261-268
Expression of Antibody Single-Chain Fragments with Specificities to Structural and Nonstructural Proteins of Beet Necrotic Yellow Vein Virus in Plants: An Alternative Approach To Produce Virus Resistant Plants....Pages 269-274
Gene Transfer in Aspen....Pages 275-281
Manufacture of Proteins Based on Recombinant Chinese Hamster Ovary Cells: Assessment of Genetic Issues and Assurance of Consistency and Quality....Pages 283-301
Front Matter....Pages 303-303
Out in the Open: Field Trials with Genetically Modified Crop Plants in Four European Countries, Chile and the United States....Pages 305-317
How Will Transgenic Sugar Beets Behave in Natural Plant Communities....Pages 319-329
Back Matter....Pages 331-335