Transgenic Ascidians

Front Matter ....Pages i-x
 Introduction  (Yasunori Sasakura)....Pages 1-3
 Microinjection of Exogenous Nucleic Acids into Eggs: Ciona Species  (Kenji Kobayashi, Yutaka Satou)....Pages 5-13
 Practical Guide for Ascidian Microinjection: Phallusia mammillata  (Hitoyoshi Yasuo, Alex McDougall)....Pages 15-24
 Microinjection of Exogenous DNA into Eggs of Halocynthia roretzi  (Gaku Kumano)....Pages 25-35
 Electroporation in Ascidians: History, Theory and Protocols  (Robert W. Zeller)....Pages 37-48
 The Use of cis-Regulatory DNAs as Molecular Tools  (Kotaro Shimai, Takehiro G. Kusakabe)....Pages 49-68
 Reporter Analyses Reveal Redundant Enhancers that Confer Robustness on Cis-Regulatory Mechanisms  (Shigeki Fujiwara, Cristian Cañestro)....Pages 69-79
 Investigating Evolutionarily Conserved Molecular Mechanisms Controlling Gene Expression in the Notochord  (Julie E. Maguire, Aakarsha Pandey, Yushi Wu, Anna Di Gregorio)....Pages 81-99
 Purification of Fluorescent Labeled Cells from Dissociated Ciona Embryos  (Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen)....Pages 101-107
 Germline Transgenesis in Ciona  (Yasunori Sasakura)....Pages 109-119
 The Enhancer Trap in Ciona  (Yasunori Sasakura)....Pages 121-129
 TALEN-Based Knockout System  (Keita Yoshida, Nicholas Treen)....Pages 131-139
 CRISPR Knockouts in Ciona Embryos  (Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi)....Pages 141-152
 Transgenic Techniques for Investigating Cell Biology During Development  (Christina D. Cota)....Pages 153-164
 Cellular Processes of Notochord Formation  (William C. Smith)....Pages 165-177
 Morphology and Physiology of the Ascidian Nervous Systems and the Effectors  (Atsuo Nishino)....Pages 179-196
Back Matter ....Pages 197-201