The Use of Resealed Erythrocytes as Carriers and Bioreactors

Front Matter....Pages i-xiii
Front Matter....Pages 1-1
On Red Blood Cells, Hemolysis and Resealed Ghosts....Pages 1-15
Front Matter....Pages 17-17
IHP Entrapment into Human Erythrocytes: Comparison between Hypotonic Dialysis and DMSO Osmotic Pulse....Pages 19-26
Density Gradient Separation of Inositol Hexaphosphate Loaded Red Blood Cells in Various Preparation Conditions....Pages 27-33
Quantitative IHP Determination by 31 P - NMR: Proposal for a Standardized Protocol....Pages 35-39
Determination of Inositol Hexaphosphate (IHP) in Human IHP-Loaded Red Blood Cells by a Simple High Performance Liquid Chromatography Method....Pages 41-49
Determination of Purity of Commercially Available Inositol Hexaphosphate (Phytates) and Preparation of a Reference Material....Pages 51-54
Standardization of an Encapsulation System: A Method to Remove Fragile Cells....Pages 55-62
The Dialysis Encapsulation Process: The Role of Endocytosis....Pages 63-71
Use of Fluorescent Probes for Describing the Process of Encapsulation by Hypotonic Dialysis....Pages 73-80
Properties of Hypotonized, Crosslinked and Crosslinked- Permeabilized Rat Erythrocytes as Potential Carrier Systems....Pages 81-89
Membrane Properties of Senescent and Carrier Human Erythrocytes....Pages 91-99
Studies with Biotinylated RBC: (1) Use of Flow Cytometry to Determine Posttransfusion Survival and (2) Isolation Using Streptavidin Conjugated Magnetic Beads....Pages 101-107
Front Matter....Pages 109-109
Resealing of Protein Tyrosine Kinase Substrates into Human Erythrocytes by Rapid Freezing and Thawing in Liquid Nitrogen....Pages 111-117
The Metabolic Role of Glucose 1,6-P 2 in Human Erythrocytes Studied by Encapsulation Procedures....Pages 119-126
The Application of Newer Concepts of Oxygen Transport to the Evaluation of the Potential Utility of Reduced Hb-O 2 Affinity....Pages 127-131
Single Cell Analysis of Factors Increasing the Survival of Resealed Erythrocytes in the Circulation of Mice....Pages 133-138
Entrapment of Purified α-Hemoglobin Chains in Normal Erythrocytes as a Model for Human β Thalassemia....Pages 139-148
ATP Monitoring in Human Red Blood Cells with Luciferase Introduced Intracellularly....Pages 149-156
Front Matter....Pages 157-157
Antagonism of the Lethal Effects of Cyanide with Resealed Erythrocytes Containing Rhodanese and Thiosulfate....Pages 159-163
Acetaldehyde Oxidation by Aldehyde Dehydrogenase Loaded Erythrocytes....Pages 165-173
Front Matter....Pages 157-157
Immunological Response to L-Asparaginase Loaded into Red Blood Cells....Pages 175-182
Normalization of Hyperglycemia in Diabetic Mice by Enzyme-Loaded Erythrocytes....Pages 183-188
Comparison of Uricase-Bound and Uricase-Loaded Erythrocytes as Bioreactors for Uric Acid Degradation....Pages 189-194
Xenobiotic Detoxification by GSH-Loaded Erythrocytes....Pages 195-201
A New Chemotherapeutic Agent: L-Asparaginase Entrapped in Red Blood Cells....Pages 203-206
Front Matter....Pages 207-207
Rubomycin Loaded Erythrocytes in the Treatment of Mouse Tumor P388....Pages 209-213
Erythrocytes as Carriers of New Anti-Opioid Prodrugs: In Vitro Studies....Pages 215-221
Interactions of Carboplatin with Human Erythrocytes and Murine Erythroleukemic Cells....Pages 223-232
Modulation of Thiopurine Metabolism in Native Human Erythrocytes....Pages 233-238
Red Blood Cells as Advanced Drug Delivery Systems for Antiviral Nucleoside Analogues....Pages 239-245
An Optimal Control Problem for the Administration of a Drug by Using Red Blood Cells as Bioreactors....Pages 247-253
Front Matter....Pages 255-255
Targeting of Eythrocytes to Cytotoxic T-Cells....Pages 257-267
Introduction to in Vivo Targeting....Pages 269-277
Erythrocytes as Carriers of Ricin a Chain: Effects on the Erythrophagocytic Cells....Pages 279-289
In Vitro Targeting of Doxorubicin Loaded Canine Erythrocytes to Cytotoxic T-Lymphocytes (CTLL)....Pages 291-297
Pharmacokinetics of Doxorubicin Loaded and Glutaraldehyde Treated Erythrocytes in Healthy and Lymphoma Bearing Dogs....Pages 299-304
Front Matter....Pages 305-305
Use of Glutaraldehyde Treated Autologous Human Erythrocytes for Hepatic Targeting of Doxorubicin....Pages 307-317
Clinical Evaluation of Glutaraldehyde-Treated Canine Erythrocytes in Normal Dogs....Pages 319-324
Sickle Cells Modified by an Osmotic Pulse in the Presence of Inositol Hexaphosphate Have Decreased Intracellular Hemoglobin Concentration and Decreased in Vitro Sickling without Prolonged in Vivo Survival....Pages 325-331
A Baboon Model to Test Physiological and Adverse Effects of Human Red Cells Loaded with Inositol Hexaphosphate (IHP)....Pages 333-340
Front Matter....Pages 305-305
Susceptibility of Carrier Erythrocytes to a Natural Hemolytic System....Pages 341-345
Gd-DOTA Loaded into Red Blood Cells, a New Magnetic Resonance Imaging Contrast Agents for Vascular System....Pages 347-354
Back Matter....Pages 355-361