The Plant Cell Wall: Methods and Protocols

Front Matter ....Pages i-xvii
 High-Voltage Paper Electrophoresis (HVPE)  (Stephen C. Fry)....Pages 1-31
 Carbohydrate Gel Electrophoresis  (Florence Goubet, Paul Dupree, Katja Salomon Johansen)....Pages 33-44
 Capillary Electrophoresis with Detection by Laser-Induced Fluorescence  (Andrew Mort, Xiangmei Wu)....Pages 45-56
 Theory and Practice in Measuring In-Vitro Extensibility of Growing Plant Cell Walls  (Daniel J. Cosgrove)....Pages 57-72
 Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Komagataeibacter Fermentation  (Deirdre Mikkelsen, Patricia Lopez-Sanchez, Dongjie Wang, Michael J. Gidley)....Pages 73-87
 Plant Tissue Cultures  (Anna Kärkönen, Arja Santanen, Kuninori Iwamoto, Hiroo Fukuda)....Pages 89-109
 Protoplast Isolation and Manipulation in the Unicellular Model Plant Penium margaritaceum  (David S. Domozych, Eleanore Ritter, Anna Lietz, Berke Tinaz, Sandra C. Raimundo)....Pages 111-124
 Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens  (Alison W. Roberts, Christos S. Dimos, Michael J. Budziszek Jr, Chessa A. Goss, Virginia Lai, Arielle M. Chaves)....Pages 125-144
 Expression of Cell Wall–Modifying Enzymes in Aspen for Improved Lignocellulose Processing  (Marta Derba-Maceluch, Ewa J. Mellerowicz)....Pages 145-164
 Activity and Action of Cell-Wall Transglycanases  (Lenka Franková, Stephen C. Fry)....Pages 165-192
 Screening for Cellulolytic Plant Enzymes Using Colorimetric and Fluorescence Methods  (Kirsten Krause, Stian Olsen)....Pages 193-201
 Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls  (Stefan J. Hill, Tracey J. Bell, Laurence D. Melton, Philip J. Harris)....Pages 203-223
 High-Resolution Imaging of Cellulose Organization in Cell Walls by Field Emission Scanning Electron Microscopy  (Yunzhen Zheng, Gang Ning, Daniel J. Cosgrove)....Pages 225-237
 Analyzing Plant Cell Wall Ultrastructure by Scanning Near-Field Optical Microscopy (SNOM)  (Tobias Keplinger, Ingo Burgert)....Pages 239-249
 Raman Imaging of Plant Cell Walls  (Batirtze Prats Mateu, Peter Bock, Notburga Gierlinger)....Pages 251-295
 Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy  (Ricardo M. F. da Costa, William Barrett, José Carli, Gordon G. Allison)....Pages 297-313
 Rapid Assessment of Cell Wall Polymer Distribution and Surface Topology of Arabidopsis Seedlings  (Mary L. Tierney, Li Sun, David S. Domozych)....Pages 315-325
 Analysis of Plant Cell Walls Using High-Throughput Profiling Techniques with Multivariate Methods  (John P. Moore, Yu Gao, Anscha J. J. Zietsman, Jonatan U. Fangel, Johan Trygg, William G. T. Willats et al.)....Pages 327-337
 Vibratome Sectioning of Plant Materials for (Immuno)cytochemical Staining  (Olivier Leroux)....Pages 339-350
 Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae  (Delphine Duffieux, Susan E. Marcus, J. Paul Knox, Cécile Hervé)....Pages 351-364
 Electron Tomography and Immunogold Labeling as Tools to Analyze De Novo Assembly of Plant Cell Walls  (Marisa S. Otegui)....Pages 365-382
 Contributions to Arabinogalactan Protein Analysis  (Romain Castilleux, Marc Ropitaux, Youssef Manasfi, Sophie Bernard, Maïté Vicré-Gibouin, Azeddine Driouich)....Pages 383-402
 In Situ/Subcellular Localization of Arabinogalactan Protein Expression by Fluorescent In Situ Hybridization (FISH)  (Mário Luís da Costa, María-Teresa Solís, Pilar S. Testillano, Sílvia Coimbra)....Pages 403-427
 Localization, Extraction, and Quantification of Plant and Algal Arabinogalactan Proteins  (Reina J. Veenhof, Zoë A. Popper)....Pages 429-441
 Plant Cell Wall Proteomes: Bioinformatics and Cell Biology Tools to Assess the Bona Fide Cell Wall Localization of Proteins  (David Roujol, Laurent Hoffmann, Hélène San Clemente, Corinne Schmitt-Keichinger, Christophe Ritzenthaler, Vincent Burlat et al.)....Pages 443-462
 Bioinformatic Identification of Plant Hydroxyproline-Rich Glycoproteins  (Xiao Liu, Savannah McKenna, Lonnie R. Welch, Allan M. Showalter)....Pages 463-481
 Bioinformatics Analysis of Plant Cell Wall Evolution  (Elisabeth Fitzek, Rhiannon Balazic, Yanbin Yin)....Pages 483-502
 Automated Glycan Assembly of Plant Cell Wall Oligosaccharides  (Fabian Pfrengle)....Pages 503-512
 Computerized Molecular Modeling of Carbohydrates  (Alfred D. French, Glenn P. Johnson)....Pages 513-539
Back Matter ....Pages 541-544