The Methodology of Plant Genetic Manipulation: Criteria for Decision Making: Proceedings of the Eucarpia Plant Genetic Manipulation Section Meeting held at Cork, Ireland from September 11 to September 14, 1994

Front Matter....Pages i-ix
Genetic manipulation in plant breeding — prospects and limitations....Pages 1-12
Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment....Pages 13-27
Transforming the plastome: genetic markers and DNA delivery systems....Pages 29-34
Genetic engineering of cereal crop plants: a review....Pages 35-44
Shoot apical meristems as a target for gene transfer by microballistics....Pages 45-51
Studies of the mechanism of transgene integration into plant protoplasts: improvement of the transformation rate....Pages 53-61
Expression of foreign genes in sunflower ( Helianthus annuus L.) — evaluation of three gene transfer methods....Pages 63-74
Maize transformation utilizing silicon carbide whiskers: a review....Pages 75-80
Transgenic barley by particle bombardment. Inheritance of the transferred gene and characteristics of transgenic barley plants....Pages 81-88
Transient gene expression in transformed banana ( Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions....Pages 89-95
Transformation of lily by Agrobacterium ....Pages 97-100
An assessment of morphogenic and transformation efficiency in a range of varieties of potato ( Solanum tuberosum L.)....Pages 101-108
Transgenic apples display stable gene expression in the fruit and Mendelian segregation of the transgenes in the R1 progeny....Pages 109-112
Transformation studies in Hordeum vulgare using a highly regenerable microspore system....Pages 113-118
Biolistic introduction of a synthetic Bt gene into elite maize....Pages 119-123
Genetic transformation of Eustoma grandiflorum Griseb. by microprojectile bombardment....Pages 125-130
Some methodological aspects of apple transformation by Agrobacterium ....Pages 131-134
Physiological complexity and plant genetic manipulation....Pages 135-147
Strategies for engineering virus resistance in transgenic plants....Pages 149-158
Resistance to tomato spotted wilt virus in transgenic tomato hybrids....Pages 159-168
Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase....Pages 169-172
Synergistic activity of chitinases and β -1,3-glucanases enhances fungal resistance in transgenic tomato plants....Pages 173-180
The sulphur-rich Brazil nut 2S albumin is specifically formed in transgenic seeds of the grain legume Vicia narbonensis ....Pages 181-192
The manipulation and modification of tomato fruit ripening by expression of antisense RNA in transgenic plants....Pages 193-202
Gene specificity is maintained in transient expression assays with protoplasts derived from different tissues of barley....Pages 203-207
Different 5′ leader sequences modulate β -glucuronidase accumulation levels in transgenic Nicotiana tabacum plants....Pages 209-216
The potential of somatic hybridization in crop breeding....Pages 217-233
Intergeneric symmetric and asymmetric somatic hybridization in Festuca and Lolium ....Pages 235-245
Transfer of disease resistance within the genus Brassica through asymmetric somatic hybridization....Pages 247-253
Microprotoplast fusion technique: a new tool for gene transfer between sexually-incongruent plant species....Pages 255-268
Improved plant heterokaryon formation by surfactant-supplementation of polyethylene glycol fusogen solution....Pages 269-273
Fluorescent in situ hybridization as an aid to introducing alien genetic variation into wheat....Pages 275-279
Sexual and somatic hybridization in the genus Lactuca ....Pages 281-285
Protoplast technology for the breeding of top-fruit trees ( Prunus, Pyrus, Malus, Rubus ) and woody ornamentals....Pages 287-294
Somaclonal variation as a tool for crop improvement....Pages 295-302
Application of in vivo and in vitro mutation techniques for crop improvement....Pages 303-315
The application of chemical mutagenesis and biotechnology to the modification of linseed ( Linum usitatissimum L.)....Pages 317-321
Modification of oilseed rape to produce oils for industrial use by means of applied tissue culture methodology....Pages 323-327
Selection of hydroxyproline-resistant proline-accumulating mutants of cauliflower ( brassica oleracea var. botrytis )....Pages 329-334
Analysis of tissue culture-derived variation in pea ( Pisum sativum L.)—preliminary results....Pages 335-339
Monitoring genetic fidelity vs somaclonal variation in Norway spruce ( Picea abies ) somatic embryogenesis by RAPD analysis....Pages 341-345
The embryogenic potential of rice cell suspensions affects their recovery following cryogenic storage....Pages 347-349
Selecting the optimum genetic background for transgenic varieties, with examples from Brassica ....Pages 351-358
Variation of transgene expression in plants....Pages 359-366
Conservation of marker synteny during evolution....Pages 367-372
 In vitro screening and field evaluation of tissue-culture-regenerated sorghum ( Sorghum bicolor (L.) Moench) for soil stress tolerance....Pages 373-380
Production and field performance of transgenic alfalfa ( Medicago sativa L.) expressing alpha-amylase and manganese-dependent lignin peroxidase....Pages 381-393
The phenotypic characterisation of R 2 generation transgenic rice plants under field and glasshouse conditions....Pages 395-401
Breeding of transgenic orange Petunia hybrida varieties....Pages 403-409
A study of different (CaMV 35S and mas ) promoter activities and risk assessment of field use in transgenic rapeseed plants....Pages 411-416
Assessing the risks of wind pollination from fields of genetically modified Brassica napus ssp. oleifera ....Pages 417-423
The release of transgenic plants from containment, and the move towards their widespread use in agriculture....Pages 425-431
The use of conventional and molecular genetics to produce new diversity in seed oil composition for the use of plant breeders — progress, problems and future prospects....Pages 433-440
Genetic engineering of Indica rice in support of sustained production of affordable and high quality food in developing countries....Pages 441-449
The integration of protoplast fusion-derived material into a potato breeding programme — a review of progress and problems....Pages 451-455
Potato germplasm enhancement for resistance to biotic stresses at CIP. Conventional and biotechnology-assisted approaches using a wide range of Solanum species....Pages 457-464
Criteria for decision making in crop improvement programmes — Technical considerations....Pages 465-476
Back Matter....Pages 477-477