The Epithelial-to Mesenchymal Transition: Methods and Protocols

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
 A Spectrum of Cell States During the Epithelial-to-Mesenchymal Transition  (Erica J. Hutchins, Marianne E. Bronner)....Pages 3-6
 Perspective on Epithelial-Mesenchymal Transitions in Embryos  (David R. McClay)....Pages 7-12
 EMT, One of Many Morphological Transitions in Cellular Phase Space  (Denise J. Montell)....Pages 13-18
 Are You Interested or Afraid of Working on EMT?  (M. Angela Nieto)....Pages 19-28
 Partial EMT/MET: An Army of One  (Sofiane Hamidi, Hiroki Nagai, Guojun Sheng)....Pages 29-33
 EMT: An Update  (Jean Paul Thiery)....Pages 35-39
Front Matter ....Pages 41-41
 Mesenchymal-to-Epithelial Transitions in Development and Cancer  (John-Poul Ng-Blichfeldt, Katja Röper)....Pages 43-62
Front Matter ....Pages 63-63
 Live Imaging of Epithelial-Mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos  (Lingkun Gu, Mo Weng)....Pages 65-77
 Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration  (Zain Alhashem, Macarena Alvarez-Garcillan Portillo, Mint Ravinand Htun, Anton Gauert, Luis Briones Montecinos, Steffen Härtel et al.)....Pages 79-106
 Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo  (Mary Cathleen McKinney, Paul M. Kulesa)....Pages 107-114
 Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors  (Sofia Golenkina, Rosemary Manhire-Heath, Michael J. Murray)....Pages 115-134
 Visualizing Mouse Embryo Gastrulation Epithelial-Mesenchymal Transition Through Single Cell Labeling Followed by Ex Vivo Whole Embryo Live Imaging  (Wallis Nahaboo, Bechara Saykali, Navrita Mathiah, Isabelle Migeotte)....Pages 135-144
 Fluorescence Recovery After Photobleaching to Study the Dynamics of Membrane-Bound Proteins In Vivo Using the Drosophila Embryo  (Joshua Greig, Natalia A. Bulgakova)....Pages 145-159
 Methods to Generate and Assay for Distinct Stages of Cancer Metastasis in Adult Drosophila melanogaster  (Jamie Adams, Andreu Casali, Kyra Campbell)....Pages 161-170
Front Matter ....Pages 171-171
 4D Live Imaging and Analysis of Chick Embryo Somites  (Gi Fay Mok, James McColl, Andrea Münsterberg)....Pages 173-181
 In Vivo Analysis of the Mesenchymal-to-Epithelial Transition During Chick Secondary Neurulation  (Elena Gonzalez-Gobartt, Guillaume Allio, Bertrand Bénazéraf, Elisa Martí)....Pages 183-197
 Multiscale In Vivo Imaging of Collective Cell Migration in Drosophila Embryos  (Gordana Scepanovic, Alexandru Florea, Rodrigo Fernandez-Gonzalez)....Pages 199-224
Front Matter ....Pages 225-225
 Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering  (Minerva Bosch-Fortea, Fernando Martín-Belmonte)....Pages 227-242
 CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay  (Sefora Conti, Takuya Kato, Danielle Park, Erik Sahai, Xavier Trepat, Anna Labernadie)....Pages 243-256
 Using Xenopus Neural Crest Explants to Study Epithelial-Mesenchymal Transition  (Nadège Gouignard, Christian Rouvière, Eric Theveneau)....Pages 257-274
 Xenopus Deep Cell Aggregates: A 3D Tissue Model for Mesenchymal-to-Epithelial Transition  (Hye Young Kim, Lance A. Davidson)....Pages 275-287
 Molecular Tension Microscopy of E-Cadherin During Epithelial-Mesenchymal Transition  (Helena Canever, Pietro Salvatore Carollo, Romain Fleurisson, Philippe P. Girard, Nicolas Borghi)....Pages 289-299
Front Matter ....Pages 301-301
 Methodologies for Following EMT In Vivo at Single Cell Resolution  (Abdull J. Massri, Geoffrey R. Schiebinger, Alejandro Berrio, Lingyu Wang, Gregory A. Wray, David R. McClay)....Pages 303-314
 Isolation and Identification of EMT Subtypes  (Robert J. Norgard, Ben Z. Stanger)....Pages 315-326
 Studying the Metabolism of Epithelial-Mesenchymal Plasticity Using the Seahorse XFe96 Extracellular Flux Analyzer  (Sugandha Bhatia, Erik W. Thompson, Jennifer H. Gunter)....Pages 327-340
 Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells  (Cecile Davaine, Eva Hadadi, William Taylor, Annelise Bennaceur-Griscelli, Hervé Acloque)....Pages 341-351
 Analysis of Cellular EMT States Using Molecular Biology and High Resolution FISH Labeling  (Noémie Kempf, Fatima Moutahir, Isabelle Goiffon, Sylvain Cantaloube, Kerstin Bystricky, Anne-Claire Lavigne)....Pages 353-383
 Mathematical Modeling of Plasticity and Heterogeneity in EMT  (Shubham Tripathi, Jianhua Xing, Herbert Levine, Mohit Kumar Jolly)....Pages 385-413
 Control of Cell Migration Using Optogenetics  (Leo Valon, Simon de Beco)....Pages 415-425
Back Matter ....Pages 427-429