Target Identification and Validation in Drug Discovery: Methods and Protocols

Front Matter ....Pages i-xiii
Front Matter ....Pages 1-1
 Using Functional Genetics in Haploid Cells for Drug Target Identification  (Jennifer C. Volz, Nicole Schuller, Ulrich Elling)....Pages 3-21
 Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug–Target Interactions  (Moritz Horn, Franziska Metge, Martin S. Denzel)....Pages 23-31
 Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development  (Konstantinos Drosopoulos, Spiros Linardopoulos)....Pages 33-42
 CellProfiler and KNIME: Open-Source Tools for High-Content Screening  (Martin Stöter, Antje Janosch, Rico Barsacchi, Marc Bickle)....Pages 43-60
Front Matter ....Pages 61-61
 In Silico Target Druggability Assessment: From Structural to Systemic Approaches  (Jean-Yves Trosset, Christian Cavé)....Pages 63-88
 In Silico Drug–Target Profiling  (Jean-Yves Trosset, Christian Cavé)....Pages 89-103
 Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies  (Matthias Brand, Georg E. Winter)....Pages 105-119
 Expression of Human VH Single Domains as Fc Fusions in Mammalian Cells  (Mahmoud Abdelatti, Peter Schofield, Daniel Christ)....Pages 121-136
Front Matter ....Pages 137-137
 The Neurosphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs)  (Rossella Galli)....Pages 139-149
 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration  (Annika Osswald, Viola Hedrich, Wolfgang Sommergruber)....Pages 151-162
 Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery  (Julia C. Meier-Hubberten, Michael P. Sanderson)....Pages 163-179
Front Matter ....Pages 181-181
 In Vivo Pharmacology Models for Cancer Target Research  (Dawei Chen, Xiaoyu An, Xuesong Ouyang, Jie Cai, Demin Zhou, Qi-Xiang Li)....Pages 183-211
 Use of CRISPR/Cas9 for the Modification of the Mouse Genome  (Alexander Klimke, Steffen Güttler, Petric Kuballa, Simone Janzen, Sonja Ortmann, Adriano Flora)....Pages 213-230
 Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction  (Cyril Seillet, Gabrielle T. Belz)....Pages 231-240
 Creation of PDX-Bearing Humanized Mice to Study Immuno-oncology  (Li-Chin Yao, Ken-Edwin Aryee, Mingshan Cheng, Pali Kaur, James G. Keck, Michael A. Brehm)....Pages 241-252
 Immunophenotyping of Tissue Samples Using Multicolor Flow Cytometry  (Martina M. Sykora, Markus Reschke)....Pages 253-268
Front Matter ....Pages 269-269
 Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers  (Boye Schnack Nielsen, Kim Holmstrøm)....Pages 271-286
 Tangential Flow Filtration with or Without Subsequent Bind-Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles  (Joel Z. Nordin, R. Beklem Bostancioglu, Giulia Corso, Samir EL Andaloussi)....Pages 287-299
 Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or Rearrangements) Combined with Protein Overexpression in Clinical Samples  (Hiroaki Nitta, Brian Kelly)....Pages 301-314
Back Matter ....Pages 315-318