T. cruzi Infection: Methods and Protocols

Front Matter ....Pages i-xvii
 Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome  (João Luís Reis-Cunha, Daniella C. Bartholomeu)....Pages 1-22
 Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine Development  (Christian Teh-Poot, Eric Dumonteil)....Pages 23-34
 Transcriptome Studies in Trypanosoma cruzi Using RNA-seq  (Gonzalo Greif, Luisa Berná, Florencia Díaz-Viraqué, Carlos Robello)....Pages 35-45
 An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-exon Gene  (Alicia Majeau, Claudia Herrera, Eric Dumonteil)....Pages 47-60
 Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi  (Noelia Lander, Miguel A. Chiurillo, Roberto Docampo)....Pages 61-76
 Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes  (Carlos André Ornelas Ricart, Rayner Myr Lauterjung Queiroz, Agenor de Castro Moreira dos Santos Júnior, Sébastien Charneau)....Pages 77-87
 Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi  (Izadora Volpato Rossi, Bruno Gavinho, Marcel Ivan Ramirez)....Pages 89-104
 Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae  (María José Ferrer, Diana Patricia Wehrendt, Mariana Bonilla, Marcelo Alberto Comini, María Teresa Tellez-Iñón, Mariana Potenza)....Pages 105-118
 Homologous Expression of Glycosylphosphatidylinositol-Anchored Glycoproteins in Trypanosoma cruzi  (Virginia Balouz, Andrea C. Mesias, Camila Centeno Camean, Ivana Ducrey, Maite Mabel Lobo, Ignacio M. Durante et al.)....Pages 119-134
 Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi trans-Sialidase  (Giannina Carlevaro, Andrés B. Lantos, Gaspar E. Cánepa, María de los Milagros Cámara, Martín Somoza, Carlos A. Buscaglia et al.)....Pages 135-146
 Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and Host-Parasite Interactions  (Martin C. Taylor, Amanda F. Francisco, Shiromani Jayawardhana, Gurdip Singh Mann, Alexander I. Ward, Francisco Olmo et al.)....Pages 147-163
 Transmigration of Trypanosoma cruzi Trypomastigotes through 3D Spheroids Mimicking Host Tissues  (Matías Exequiel Rodríguez, Mariana Rizzi, Lucas Caeiro, Yamil Masip, Daniel O. Sánchez, Valeria Tekiel)....Pages 165-177
 In Situ Detection of Dormant Trypanosoma cruzi Amastigotes Using Bioluminescent-Fluorescent Reporters  (Fernando Sánchez-Valdéz, Angel Padilla)....Pages 179-186
 Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal Models  (Julien Santi-Rocca, Núria Gironès, Manuel Fresno)....Pages 187-202
 MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice  (Ludmila Rodrigues Pinto Ferreira)....Pages 203-214
 Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCR  (Juan Carlos Ramírez, Otacilio da Cruz Moreira)....Pages 215-225
 Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR  (Otacilio da Cruz Moreira, Juan Carlos Ramirez)....Pages 227-238
 Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi Trans-sialidase  (María Susana Leguizamón)....Pages 239-246
 Analytics of Functional Autoantibodies in Patients with Chagas Disease  (Gerd Wallukat, Katrin Wenzel, Ingolf Schimke)....Pages 247-261
 Apolipoprotein A1 and Fibronectin Fragments as Markers of Cure for the Chagas Disease  (Elizabeth Ruiz-Lancheros, Makan Golizeh, Momar Ndao)....Pages 263-273
 Host-Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in Plasma  (Julio Alonso-Padilla, Dolors Tassies, Nuria Cortes-Serra, Joaquim Gascon, Joan-Carles Reverter, María-Jesús Pinazo)....Pages 275-286
 Purification of Glycosylphosphatidylinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of α-Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease  (Uriel Ortega-Rodriguez, Susana Portillo, Roger A. Ashmus, Jerry A. Duran, Nathaniel S. Schocker, Eva Iniguez et al.)....Pages 287-308
 Cytometric Bead Array (CBA) for Measuring Cytokine Levels in Chagas Disease Patients  (Nayara I. Medeiros, Juliana A. S. Gomes)....Pages 309-314
 Generation of T. cruzi-Specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients  (Gonzalo R. Acevedo, Paula B. Alcaráz, Clemencia Pinilla, Karina A. Gómez)....Pages 315-337
 Trypanosoma cruzi-Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas Disease  (María Cecilia Albareda, María Ailén Natale, Gonzalo Leandro Cesar, Melisa Daiana Castro Eiro, María Gabriela Alvarez, Susana Adriana Laucella)....Pages 339-348
 CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas Disease  (Paola Lasso, Jose Mateus, John Mario González, Adriana Cuéllar, Concepción Puerta)....Pages 349-361
 Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease  (John E. Wiktorowicz, M. Paola Zago, Nisha J. Garg)....Pages 363-380
 Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected Hearts  (Natalia Eberhardt, Liliana María Sanmarco, Maria Pilar Aoki)....Pages 381-395
Back Matter ....Pages 397-398