T-Cell Motility: Methods and Protocols

Front Matter ....Pages i-x
 An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility  (Navin Kumar Verma, Dermot Kelleher)....Pages 1-9
 Isolation of Human Peripheral Blood T-Lymphocytes  (Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Navin Kumar Verma)....Pages 11-17
 A Laboratory Model to Study T-Cell Motility  (Navin Kumar Verma, Shyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan et al.)....Pages 19-23
 Quantification of T-Cell Migratory Phenotypes Using High-Content Analysis  (Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, Navin Kumar Verma)....Pages 25-32
 Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time  (Seow Theng Ong, Navin Kumar Verma)....Pages 33-40
 Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signaling Cross-Talks in Motile T-Cells  (Seow Theng Ong, Graham D. Wright, Navin Kumar Verma)....Pages 41-50
 Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis  (Praseetha Prasannan, Navin Kumar Verma)....Pages 51-57
 Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes  (Atish Kizhakeyil, Navin Kumar Verma)....Pages 59-66
 GapmeR-Mediated Gene Silencing in Motile T-Cells  (Mobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Somaraju Chalasani, Atish Kizhakeyil, Navin Kumar Verma)....Pages 67-73
 Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices Using Confocal Microscopy  (Emmanuel Donnadieu, Yvonne Michel, Martin-Leo Hansmann)....Pages 75-82
 Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes  (Praseetha Prasannan, Rathi Saravanan, Navin Kumar Verma)....Pages 83-90
 Immunometabolomic Phenotyping of Motile T-Cells  (Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, Navin Kumar Verma)....Pages 91-98
 Profiling Activity of Cellular Kinases in Migrating T-Cells  (Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy et al.)....Pages 99-113
 Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes  (Malin Samuelsson, Lena M. Svensson)....Pages 115-122
 A Protocol to Study T-Cell Signaling in an Immune Synapse by Microscopy  (Mobashar Hussain Urf Turabe Fazil, Pankaj Kumar, Navin Kumar Verma)....Pages 123-128
 Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides  (Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, Navin Kumar Verma)....Pages 129-138
 Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility  (Geoffrey W. Payne, Kevin Mitchell, Stephanie L. Sellers)....Pages 139-147
 Computational Analysis of Protein–Protein Interactions in Motile T-Cells  (Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khurshid Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, Navin Kumar Verma)....Pages 149-156
Back Matter ....Pages 157-158