Stability and Stabilization of Enzymes

Content: 
Front Matter
Page iii

Copyright page
Page iv

Studies in Organic Chemistry
Pages v-vi

Preface
Page vii

Acknowledgements
Page viii

The behaviour of proteins at interfaces in relation to their structural stability
Pages 3-11
Willem Norde

Influence of the solvent properties on protein stability in organic media
Pages 13-20
E. Battistel, D. Bianchi

Interfacial damage to proteins during intensive mixing in fermentation and downstream processing
Pages 21-27
M. Hoare, M.R. Khan, P. Dunnill

Thermal stability of enzymes: influence of solvatation medium (a Raman spectroscopy study)
Pages 29-36
D. Combes, I. Auzanneau, A. Zwick

Protein Stability in Non-Aqueous Media: A DSC Study
Pages 37-44
G. Barone, P. Del Vecchio, D. Fessas, C. Giancola, G. Graziano, A. Riccio

Stable enzymes by water removal
Pages 45-54
Felix Franks, Ross H.M. Hatley

Enzyme Stabilization by Multipoint Covalent Attachment to Activated Pre-Existing Supports
Pages 55-62
J.M. Guisán, R. Fernandez-Lafuente, V. Rodriguez, A. Bastida, R.M. Blanco, G. Alvaro

Crosslinked enzyme crystals (CLECs™) as immobilized enzyme particles
Pages 63-73
M.A. Navia, N.L.St. Clair, J.P. Griffith

Stabilization of proteins by chemical methods
Pages 75-80
Vadim V. Mozhaev

Stability of engineered antibody fragments
Pages 81-90
Andreas Plückthun

Structural Determinants of the Thermostability of thermolysin-Like Bacillus Neutral Proteases
Pages 91-99
V.G.H. Eijsink, G. Vriend, F. Hardy, O.R. Veltman, B. Van der Vinne, B. van den Burg, B.W. Dijkstra, J.R. van der Zee, G. Venema

Molecular Aspects of Proteolysis of Globular Proteins
Pages 101-110
Angelo Fontana, Patrizia Polverino de Laureto, Vincenzo De Filippis

Stability of Industrial Enzymes
Pages 111-131
O. Misset

Stabilization of the Detergent Protease Savinase® by Proline Substitution
Pages 133-144
C. von der Osten, L. Hedegaard, P. Østergaard, C. Lauridsen, L. Kierstein, S. Branner

Lessons from Industry
Pages 145-151
K. Sauber

Stabilization of Enzymes by their Specific Antibodies
Pages 153-163
E.Y. Shami, M. Ramjeesingh, A. Rothstein, M. Zywulko

Immobilisation of α-Chymotrypsin on soluble acrylic microgels; activity and stabilisation
Pages 167-174
A.R. Alcántara, J.V. Sinisterra, C. Torres, P. Romanelli, A. Williams

Improved Stability of Lignin Peroxidase by Immobilization
Pages 175-180
Michèle ASTHER, Jean-Claude MEUNIER

Ca2+-induced Enhancement of the Molecular Stability of Pseudomonas Lipases
Pages 181-188
M. Barfoed, K. Borch, H. Lund, S.A. Patkar

Denaturation of Ribonucleases from Different Sources in the Presence of Denaturing or Stabilizing Agents
Pages 189-196
G. Barone, P. Del Vecchio, D. Fessas, C. Giancola, G. Graziano, A. Riccio

Conformational dynamics of native, compact and fully unfolded states of proteins detected by frequency domain fluorometry
Pages 197-204
Ettore Bismuto, Ivana Sirangelo, Enrico Gratton, Gaetano Irace

Characteristics, protein engineering and applications of psychrophilic marine proteinases from Atlantic cod
Pages 205-214
J.B. Bjarnason, B. Ásgeirsson, M.M. Kristjánsson, Á. Guðmundsdóttir, J.W. Fox, J.F. Chlebowski, C.S. Craik

Low Temperature Inactivation of A Bacterial Protease
Pages 215-222
M.A.J.S. van Boekel

Chemical deglycosylation of Horseradish peroxidase and surglycosylation using a new glycosylating reagent: effects on catalytic activity and stability
Pages 223-229
D. BONNAFFE, M. THERISOD, J.L. SERIS, A. LUBINEAU

Engineering stability and specificity of the Lactococcus lactis SK11 proteinase
Pages 231-238
P.G. Bruinenberg, P. Vos, F.A. Exterkate, A.C. Alting, W.M. de Vos, R.J. Siezen

Comparative studies on the thermophilicity and stability of 5′-methylthioadenosine phosphorylase from various sources
Pages 239-246
G. Cacciapuoti, M. Porcelli, C. Bertoldo, S. Fusco, V. Zappia

A Process for Stabilization of Glycoproteins
Pages 247-253
V. Česi, B. Kozulić, S. Barbarić

Pressure - Induced Structural Modifications of Butyrylcholinesterase
Pages 255-260
C. CLERY, K. GOOSSENS, G. HUI BON HOA, K. HEREMANS, C. BALNY, P. MASSON

Changing the Thermostability of Bacillus Licheniformis α-amylase
Pages 261-268
S. De Cordt, J. Saraiva, M. Hendrickx, G. Maesmans, P. Tobback

Stabilization of Lipases for Hydrolysis Reactions on Industrial Scale
Pages 269-274
F.P. Cuperus, S.Th. Bouwer, A.M. Krosse, J.T.P. Derksen

Cold denaturation of proteins as investigated by subzero transverse temperature gradient gel electrophoresis
Pages 275-282
Christophe Curtil, Patrick Masson

Genetic algorithms as a new tool to study protein stability
Pages 283-290
Thomas Dandekar, Patrick Argos

Modeling three-dimensional structure and electrostatics of alkali-stable cyclomaltodextrin glucanotransferase
Pages 291-298
E. Demchuk, M. Vihinen, R. Wade, T. Korpela

The effect of metal ion binding on protein stability
Pages 299-307
J. Desmet, M. Joniau, H. Van Dael

The number of cooperative regions (energetical domains) in a pepsin molecule depends on the pH of the medium
Pages 309-314
N. Esipova, I. Grishina, I. Protasevich, E. Frank, V. Lobachov, A. Makarov

Stabilization of Soluble Proteins by Intramolecular Crosslinking with Polyfunctional Macromolecules. Poly-(Glutaraldehyde-Like) Structure
Pages 315-322
R. Fernández-Lafuente, V. Rodríguez, A. Bastida, R.M. Blanco, G. Alvaro, J.M. Guisán

Development of A Method for the Stabilization and Formulation of Xylanase from Trichoderma Using Experimental Design
Pages 323-328
R. Spencer Fisk, Curran Simpson

New technique for monitoring interfacial inactivation of enzymes by organic solvents
Pages 329-336
Amreek S. Ghatorae, Maria J. Guerra, George Bell, Peter J. Halling

The Stabilisation of Analytical Enzymes Using Polyelectrolytes and Sugar Derivatives
Pages 337-346
T.D. Gibson, J.N. Hulbert, B. Pierce, J.I. Webster

Pressure effect on the stability of Lipoxygenase: FTIR studies with the diamond anvil cell
Pages 347-352
K. Goossens, L. Smeller, K. Heremans, J. Frank

Stability of Ca2+-binding mutants of human lysozyme
Pages 353-360
P. Haezebrouck, I. Hanssens, M. Joniau, H. Van Dael

Storage stability of enzymes in dry apolar solvent
Pages 361-367
Rajni Kaul, Bo Mattiasson

Pressure Sensitivity of Enzymes and Their Modification; Proteolytic Modification of Metallo Protease under High Pressure
Pages 369-375
Shigeru Kunugi, Yukiko Murakami, Akihiko Nomura

Comparative study of thermostability and structure of close homologs – barnase and binase
Pages 377-382
A. Makarov, I. Protasevich, N. Kuznetsova, B. Fedorov, S. Korolev, N. Struminskaya, I. Leshchinskaya, G. Yakovlev, N. Esipova

Immobilized Concanavalin A decreases the stability at proteolysis of amineoxidases
Pages 383-389
M.A. Mateescu, O. Befani, B. Mondovi

Stabilisation of lipase from Candida rugosa by covalent immobilisation
Pages 391-398
J.M. Moreno, M. Arroyo, J.V. Sinisterra

Isolation, characterization and immobilization of penicillin acylase from Escherichia coli B-130
Pages 399-405
S. Muliawati, N. Mulyani

Thermodynamic properties of apocytochrome P450cam
Pages 407-414
W. Pfeil, B. Nölting, C. Jung

Stabilization of Yeast D-Amino Acid Oxidase by Matrix Covalent Attachment
Pages 415-420
M.S. Pilone, L. Pollegioni, S. Butò

Stability of an entrapped-cell system for the Δl-dehydrogenation of steroids in organic medium
Pages 421-428
H.M. Pinheiro, J.M.S. Cabral

The kinetics of enzyme inactivation
Pages 429-435
Guido Greco jr., Domenico Pirozzi, Michele Maremonti, Giuseppe Toscano, Liliana Gianfreda

Studies on stability of S-adenosylhomocysteine hydrolase from Sulfolobus solfataricus, a thermophilic archaebacterium
Pages 437-444
M. Porcelli, G. Cacciapuoti, S. Fusco, C. Bertoldo, V. Zappia

Temperature, pH and Media Influence on Lipase Stability
Pages 445-450
D.M.F. Prazeres, F.A.P. Garcia, J.M.S. Cabral

Prediction of the unfolding heat capacity change of proteins
Pages 451-457
R. Ragone, G. Colonna

Inactivation of α-amylase from Bacillus amyloliquefaciens at low moisture contents
Pages 459-466
J. Saraiva, S. De Cordt, M. Hendrickx, J. Oliveira, P. Tobback

Kinetics of High-Temperature Inactivation of Extracellular Protease from Pseudomonas Fluorescens 22F
Pages 467-472
E.P. Schokker, M.A.J.S. van Boekel

Stability of a Fusarium solani pisi recombinant cutinase in reversed micelles
Pages 473-480
M.J. Sebastião, J.M.S. Cabral, M.R. Aires-Barros

Influence of long chain alcohols and chemical modification on the microencapsulated α-chymotrypsin stability
Pages 481-488
M.L.M. Serralheiro, J.M.S. Cabral

Stability of A. Oryzae ß-Galactosidase in Water Miscible Organic Solvents
Pages 489-496
P.V. Sundaram, S. Shubhada

Fixation of the unfolding region - a hypothesis of enzyme stabilization
Pages 497-504
R. Ulbrich-Hofmann, R. Golbik, W. Damerau

Probing Conformational Transitions in Interface α1β2 of Human Hemoglobin by Site-Directed Mutagenesis
Pages 505-509
B. Vallone, V. Cavalli, M. Brunori

Chemical modification of the protein molecules improve their activity in organic solvents
Pages 511-517
R. Vazquez-Duhalt, P.M. Fedorak, D.W.S. Westlake

Author Index
Pages 519-520