Short-Term Tests for Chemical Carcinogens

Front Matter....Pages i-xviii
A Biochemical Phage Induction Assay for Carcinogens....Pages 1-11
Detection of DNA-Modifying Agents by Analyzing the Lesions Introduced into Purified PM2 DNA....Pages 12-19
Reactivation of Viruses....Pages 20-35
Metabolic Activation of Nitroheterocyclic Compounds in Bacteria and Mammalian Cells....Pages 36-47
Utilization of the Alkaline Elution Assay as a Short-Term Test for Chemical Carcinogens....Pages 48-58
DNA Synthesis Inhibition in Mammalian Cells as a Test for Mutagenic Carcinogens....Pages 59-64
DNA Repair Synthesis (UDS) as an in vitro and in vivo Bioassay to Detect Precarcinogens, Ultimate Carcinogens, and Organotropic Carcinogens....Pages 65-82
Multi-well Assay for Unscheduled DNA Synthesis Using Human Diploid Fibroblasts....Pages 83-89
Sucrose Gradients: An Assay for DNA Damage....Pages 90-93
The Testicular DNA-Synthesis Inhibition Test (DSI Test)....Pages 94-107
The Salmonella Mutagenicity Test: An Overview....Pages 108-119
Applications of the Salmonella /Microsome Assay....Pages 120-126
Determination of Genotoxic Activity Using DNA Polymerase-Deficient and -Proficient E. coli ....Pages 127-139
The Nucleotide-Permeable Escherichia coli : A Model System that Responds to DNA-Binding Carcinogens, Mutagens, and Antitumor Agents with DNA Excision Repair or with Inhibition of Replicative DNA Synthesis....Pages 140-160
The Yeast Saccharomyces cerevisiae : An Assay Organism for Environmental Mutagens....Pages 161-170
Induction and Genetic Characterization of Specific Locus Mutations in the ad-3 Region in Two-Component Heterokaryons of Neurospora crassa ....Pages 175-186
Neurospora and Environmentally Induced Aneuploidy....Pages 187-199
Plant Genetic Test Systems for the Detection of Chemical Mutagens....Pages 200-216
A Short-Term Cytogenetic Test for Genetic Instability in Humans....Pages 217-235
The Sister Chromatid Exchange Test....Pages 236-242
The Micronucleus Assay. I. In Vivo ....Pages 243-249
The Micronucleus Assay. II. In Vitro ....Pages 250-254
Automation in Cytogenetics....Pages 255-263
Mutagenesis Studies in Diploid Human Cells with Different DNA-Repair Capacities....Pages 264-276
Liver Culture Indicators for the Detection of Chemical Carcinogens....Pages 277-289
An Escherichia coli Differential Killing Test for Carcinogens Based on a uvrA recA lexA Triple Mutant....Pages 290-295
Detection of Carcinogens Using the Fluctuation Test with S9 or with Hepatocyte Activation....Pages 296-305
 In vitro Mammalian Cell Transformation for Identification of Carcinogens, Cocarcinogens, and Anticarcinogens....Pages 306-322
The Use of Cryopreserved Syrian Hamster Embryo Cells in a Transformation Test for Detecting Chemical Carcinogens....Pages 323-337
Assay of Chemically Induced Transformation of Human Cells....Pages 338-349
Chemical— Viral Interactions: Enhancement of Viral Transformation by Chemical Carcinogens....Pages 350-361
The Calcium-Independence of Neoplastic Cell Proliferation: A Promising Tool for Carcinogen Detection....Pages 362-371
Induction of a Resistant Preneoplastic Liver Cell as a New Principle for a Short-Term Assay in vivo for Carcinogens....Pages 372-378
Recent Achievements with Drosophila as an Assay System for Carcinogens....Pages 379-398
Strategy for Breeding Test Animals of High Susceptibility to Carcinogens....Pages 399-407
Methods for Human and Murine Sperm Assays....Pages 408-419
 In vitro Assay for Tumor Promoters....Pages 420-427
Inhibition of Chemical Mutagenesis: An Application of Chromosome Aberration And DNA Synthesis Assays Using Cultured Mammalian Cells....Pages 428-437
Detection of Cocarcinogens and Anticarcinogens with Microbial Mutagenicity Assays....Pages 438-448
The Use of a Bacterial Assay to Identify Which Agents Modify Carcinogen-Induced Mutagenesis....Pages 449-456
Quantitative Measures of Induced Mutagenesis....Pages 457-473
Tests for Potential Carcinogens: Unresolved Problems....Pages 474-482
Mutagenicity Testing: Problems in Application....Pages 483-504
Short-Term Genetic Tests Extended to the Human....Pages 505-510
Back Matter....Pages 511-518