SELENOPROTEINS : methods and protocols

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
 SECISearch3 and Seblastian: In-Silico Tools to Predict SECIS Elements and Selenoproteins  (Marco Mariotti)....Pages 3-16
 Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins  (Didac Santesmasses, Marco Mariotti, Roderic Guigó)....Pages 17-28
 SelGenAmic: An Algorithm for Selenoprotein Gene Assembly  (Liang Jiang, Qiong Liu)....Pages 29-39
Front Matter ....Pages 41-41
 Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencing  (Bradley A. Carlson, Byeong Jae Lee, Petra A. Tsuji, Paul R. Copeland, Ulrich Schweizer, Vadim N. Gladyshev et al.)....Pages 43-60
 Identification and Characterization of Proteins that Bind to Selenoprotein 3′ UTRs  (Eric M. Cockman, Donna M. Driscoll)....Pages 61-71
 Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis  (Olga Kossinova, Alexey Malygin, Alain Krol, Galina Karpova)....Pages 73-92
 In Vitro Translation Assays for Selenocysteine Insertion  (Mark H. Pinkerton, Paul R. Copeland)....Pages 93-101
 Studying Selenoprotein mRNA Translation Using RNA-Seq and Ribosome Profiling  (Brian K. Dalley, Lisa Baird, Michael T. Howard)....Pages 103-123
 Modification of Selenoprotein mRNAs by Cap Tri-methylation  (Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang)....Pages 125-141
Front Matter ....Pages 143-143
 Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)  (Véronique Vacchina, Jean Dumont)....Pages 145-152
 Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP MS)  (Véronique Vacchina, Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski)....Pages 153-162
 Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum  (Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera, José Luis Gómez-Ariza)....Pages 163-175
 Radioactive 75Se Labeling and Detection of Selenoproteins  (Sun Hee Yim, Ryuta Tobe, Anton A. Turanov, Bradley A. Carlson)....Pages 177-192
 Nonradioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines  (Jordan Sonet, Sandra Mounicou, Laurent Chavatte)....Pages 193-203
 Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips  (Jordan Sonet, Sandra Mounicou, Laurent Chavatte)....Pages 205-217
 Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues  (Elisa Castañeda Santa Cruz, J. Susanne Becker, J. Sabine Becker, Alessandra Sussulini)....Pages 219-227
Front Matter ....Pages 229-229
 Overexpression of Recombinant Selenoproteins in E. coli  (Qing Cheng, Elias S. J. Arnér)....Pages 231-240
 Preparation of Selenocysteine-Containing Forms of Human SELENOK and SELENOS  (Zhengqi Zhang, Jun Liu, Sharon Rozovsky)....Pages 241-263
 Selenocysteine-Mediated Expressed Protein Ligation of SELENOM  (Jun Liu, Qingqing Chen, Sharon Rozovsky)....Pages 265-283
 Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity  (Lionel Tarrago, Emmanuel Oheix, Zalán Péterfi, Vadim N. Gladyshev)....Pages 285-299
 Selective Evaluation of Thioredoxin Reductase Enzymatic Activities  (Elias S. J. Arnér)....Pages 301-309
Front Matter ....Pages 311-311
 Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk  (Catherine Méplan)....Pages 313-324
 Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis  (Erik Schoenmakers, Krishna Chatterjee)....Pages 325-335
Back Matter ....Pages 337-340