RNA Processing Part B: Specific Methods

Content: 
Contributors to volume 181
Pages ix-xii

Preface
Pages xiii-xiv
James E. Dahlberg, John N. Abelson

Volumes in series
Pages xv-xxix

Preparation and fractionation of mammalian extracts active in pre-mRNA splicing Original Research Article
Pages 3-19
Angela Krämer, Walter Keller

Small-scale preparation of extracts from radiolabeled cells efficient in pre-mRNA splicing Original Research Article
Pages 20-30
Kevin A.W. Lee, Michael R. Green

Preparation of active nuclei Original Research Article
Pages 30-36
William F. Marzluff

Nonaqueous isolation of transcriptionally active nuclei from Xenopus oocytes Original Research Article
Pages 36-43
Elsebet Lund, Philip L. Paine

Transient transfection of HeLa cells for analysis of splicing reaction Original Research Article
Pages 43-48
Markus Aebi

Preparation of mammalian extracts active in polyadenylation Original Research Article
Pages 49-74
Claire L. Moore

Histone-specific RNA 3′ processing in nuclear extracts from mammalian cells Original Research Article
Pages 74-89
Claudia Stauber, Dominique Soldati, Bernhard LГјscher, Daniel SchГјmperli

Preparation and fractionation of yeast splicing extract Original Research Article
Pages 89-96
Soo-Chen Cheng, Andrew Newman, Ren-Jang Lin, Gloria D. McFarland, John N. Abelson

Affinity chromatography with biotinylated RNAs Original Research Article
Pages 97-121
S.W. Ruby, S.E. Goelz, Z. Hostomsky, J.N. Abelson

Measurement and analysis of yeast pre-mRNA sequence contribution to splicing efficiency Original Research Article
Pages 122-147
B.C. Rymond, C. Pikielny, B. Seraphin, P. Legrain, M. Rosbash

Analysis of pre-mRNA processing in transfected plant protoplasts Original Research Article
Pages 148-161
Gregory J. Goodall, Karin Wiebauer, Witold Filipowicz

Polyadenylate polymerases Original Research Article
Pages 161-170
Mary Edmonds

Purification and use of vaccinia virus messenger RNA capping enzyme Original Research Article
Pages 170-180
Stewart Shuman, Bernard Moss

RNA lariat debranching enzyme as tool for analyzing RNA structure Original Research Article
Pages 180-188
Barbara Ruskin, Michael R. Green

Escherichia coli ribonuclease III Original Research Article
Pages 189-202
Hugh D. Robertson

Messenger RNA turnover in cell-free extracts Original Research Article
Pages 202-209
Gary Brewer, Jeffrey Ross

Nuclear cap binding protein from HeLa cells Original Research Article
Pages 209-215
Mutsuhito Ohno, Yoshiro Shimura

Purification of small nuclear ribonucleoprotein particles active in RNA processing Original Research Article
Pages 215-232
Angela Krämer

Purification of small nuclear ribonucleoprotein particles with antibodies against modified nucleosides of small nuclear RNAs Original Research Article
Pages 232-257
Montserrat Bach, Peter Bringmann, Reinhard LГјhrmann

Isolation of small nuclear ribonucleoprotein polypeptides Original Research Article
Pages 257-263
Sallie O. Hoch

Purification and characterization of U small nuclear ribonucleoproteins in cesium chloride gradients Original Research Article
Pages 264-273
Claude Brunel, Guy Cathala

Structural analysis of U small nuclear ribonucleoproteins by in vitro assembly Original Research Article
Pages 273-284
Jörg Hamm, Iain W. Mattaj

Site-specific degradation of RNA of small nuclear ribonucleoprotein particles with complementary oligodeoxynucleotides and RNase H Original Research Article
Pages 284-292
Angela Krämer

Isolation and in vitro assembly of nuclear ribonucleoprotein particles and purification of core particle proteins Original Research Article
Pages 293-307
Stanley F. Barnett, Stephanie J. Northington, Wallace M. LeStourgeon

Physical methods for characterization of heterogeneous nuclear ribonucleoprotein complexes Original Research Article
Pages 307-317
Jerzy T. Schöneich, John O. Thomas

Immunological methods for purification and characterization of heterogeneous nuclear ribonucleoprotein particles Original Research Article
Pages 317-325
SerafГ­n PiГ±ol-Roma, Yang Do Choi, Gideon Dreyfuss

Purification and characterization of proteins of heterogeneous nuclear ribonucleoprotein complexes by affinity chromatography Original Research Article
Pages 326-331
SerafГ­n PiГ±ol-Roma, Maurice S. Swanson, Michael J. Matunis, Gideon Dreyfuss

Purification and characterization of polyadenylate-binding protein Original Research Article
Pages 332-352
Alan B. Sachs, Roger D. Kornberg

Preparation of extracts and assay of ribosomal RNA maturation in Escherichia coli Original Research Article
Pages 355-366
Anand K. Srivastava, David Schlessinger

Ribosomal RNA terminal maturase: Ribonuclease M5 from Bacillus subtilis Original Research Article
Pages 366-374
Norman R. Pace, Bernadette Pace

Yeast suppressor mutations and transfer RNA processing Original Research Article
Pages 377-394
Mark Nichols, Ian Willis, Dieter Söll

Genetics of transfer RNA processing in Escherichia coli Original Research Article
Pages 395-400
Yoshiro Shimura, Hideaki Shiraishi

Genetic methods for study of trans-acting genes involved in processing of precursors to yeast cytoplasmic transfer RNAs Original Research Article
Pages 400-421
Anita K. Hopper

Ribonucleases active at 3′ terminus of transfer RNA Original Research Article
Pages 421-433
Murray P. Deutscher

Transfer RNA nucleotidyltransferase Original Research Article
Pages 434-439
Murray P. Deutscher

Yeast extracts for transfer RNA gene transcription and processing Original Research Article
Pages 439-450
Claire F. Evans, David R. Engelke

Enzymatic addition of guanylate to histidine transfer RNA Original Research Article
Pages 451-462
Jacinta B. Williams, Lynn Cooley, Dieter Söll

Purification of yeast transfer RNA ligase Original Research Article
Pages 463-471
Qi Xu, Eric M. Phizicky, Chris L. Greer, John N. Abelson

Highly purified transfer RNA splicing endonuclease from Saccharomyces cerevisiae Original Research Article
Pages 471-480
Phillip R. Green, John N. Abelson

Purification and characterization of wheat germ RNA ligase and associated activities Original Research Article
Pages 480-499
Leslie Pick, Henry M. Furneaux, Jerard Hurwitz

RNA 3′-terminal phosphate cyclase from HeLa cells Original Research Article
Pages 499-510
Witold Filipowicz, Oscar Vicente

Transfer RNA splicing endonuclease from Xenopus laevis Original Research Article
Pages 510-517
D. Gandini-Attardi, I.M. Baldi, M. Mattoccia, G.P. Tocchini-Valentini

Genetic and molecular analysis of RNA splicing in Escherichia coli Original Research Article
Pages 521-539
Marlene Belfort, Karen Ehrenman, P.Scott Chandry

Genetic analysis of RNA splicing in yeast mitochondria Original Research Article
Pages 539-558
Philip S. Perlman

Self-splicing and enzymatic cleavage of RNA by a group I intervening sequence Original Research Article
Pages 558-569
John A. Latham, Arthur J. Zaug, Thomas R. Cech

Preparation and characterization of RNase P from Escherichia coli Original Research Article
Pages 569-582
Madeline F. Baer, John G. Arnez, Cecilia Guerrier-Takada, Agustin Vioque, Sidney Altman

Characterization of self-cleavage of viroid and virusoid RNAs Original Research Article
Pages 583-607
Anthony C. Forster, Christopher Davies, Cheryl J. Hutchins, Robert H. Symons

Author index
Pages 609-631

Subject index
Pages 633-651