Reversed Phase Chromatography

Front Page......Page 1
Contents......Page 3
1. Introduction......Page 5
Theory of reveresed phase chromatography......Page 6
The matrix......Page 9
The ligands......Page 11
Resolution......Page 13
Capacity factor......Page 14
Efficiency......Page 15
Selectivity......Page 17
Binding capacity......Page 18
Flow rate......Page 19
Organic solvent......Page 20
Ion suppression......Page 21
Ion pairing agents......Page 22
Gradient elution......Page 23
Desalting......Page 24
Large scale preparative purification......Page 25
Capture......Page 26
Polishing......Page 27
2. Product Guide......Page 29
Product description......Page 30
High Chemical Stability......Page 32
Excellent Flow/Pressure Characteristics......Page 34
High Capacity......Page 35
Availability......Page 36
Product description......Page 37
Capacity......Page 38
Product description......Page 39
Availability......Page 40
Resolution......Page 41
Molecular weight of the sample components......Page 42
Class of sample components......Page 43
The organic solvent......Page 44
pH......Page 46
Ion pairing agents......Page 47
Sample preparation......Page 49
Solvent disposal......Page 50
Mobile phase balancing......Page 51
Column conditioning......Page 52
Elution conditions......Page 53
Column cleaning......Page 55
Column storage......Page 56
Designing a biochemical purification......Page 57
Naturally occurring peptides and proteins......Page 58
Trace enrichment......Page 59
Purification of cholecystokinin-58 (CCK-58) from pig intesting......Page 60
Recombinant peptides and proteins......Page 62
Purification of recombinant human epidermal growth factor......Page 63
Purification of a phosphorylated PDGF a-receptor derived peptide......Page 65
Structural characterisation of a 165 kDa protein......Page 66
Protein characterisation at the micro-scale......Page 67
Protein identification by LC-MS......Page 69
Chemically synthesised oligonucleotides......Page 70
5. Fault finding chart......Page 73
6. References......Page 81
7. Ordering Information......Page 84