Retinal Degeneration: Methods and Protocols

Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
 Identification and Analysis of Genes Associated with Inherited Retinal Diseases  (Mubeen Khan, Zeinab Fadaie, Stéphanie S. Cornelis, Frans P. M. Cremers, Susanne Roosing)....Pages 3-27
 Conduct and Quality Control of Differential Gene Expression Analysis Using High-Throughput Transcriptome Sequencing (RNASeq)  (Felix Grassmann)....Pages 29-43
 Testing for Known Retinal Degeneration Mutants in Mouse Strains  (Khalid Rashid, Katharina Dannhausen, Thomas Langmann)....Pages 45-58
 CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo  (Daniela Benati, Valeria Marigo, Alessandra Recchia)....Pages 59-74
 Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis  (Yuchen Lin, Sarah Irmscher, Christine Skerka)....Pages 75-83
Front Matter ....Pages 85-85
 Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells  (Caroline Brandl)....Pages 87-94
 Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture  (Francesca Mazzoni, Yingyu Mao, Silvia C. Finnemann)....Pages 95-108
 Porcine RPE/Choroidal Explant Cultures  (Alexa Klettner, Yoko Miura)....Pages 109-118
 The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells  (Manuela Völkner, Thomas Kurth, Mike O. Karl)....Pages 119-141
 Cell Death Analysis in Retinal Cultures  (Sarah L. Roche, Ana M. Ruiz-Lopez, Thomas G. Cotter)....Pages 143-152
 Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-Derived Macrophages in Retinal Disease  (Nancy J. Reyes, Rose Mathew, Daniel R. Saban)....Pages 153-164
Front Matter ....Pages 165-165
 Light Damage Models of Retinal Degeneration  (Christian Grimm, Charlotte E. Remé)....Pages 167-178
 Induction and Readout of Oxygen-Induced Retinopathy  (Raffael Liegl, Claudia Priglinger, Andreas Ohlmann)....Pages 179-191
 Generation and Analysis of Xenopus laevis Models of Retinal Degeneration Using CRISPR/Cas9  (Joanna M. Feehan, Paloma Stanar, Beatrice M. Tam, Colette Chiu, Orson L. Moritz)....Pages 193-207
 Gene Knockdown in Zebrafish (Danio rerio) as a Tool to Model Photoreceptor Diseases  (Holger Dill, Utz Fischer)....Pages 209-219
 Drosophila melanogaster: A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa  (Malte Lehmann, Elisabeth Knust, Sarita Hebbar)....Pages 221-249
Front Matter ....Pages 251-251
 Retinal Fundus Imaging in Mouse Models of Retinal Diseases  (Anne F. Alex, Maged Alnawaiseh, Peter Heiduschka, Nicole Eter)....Pages 253-283
 Phenotyping of Mouse Models with OCT  (G. Alex Ochakovski, M. Dominik Fischer)....Pages 285-291
 Cell-Specific Markers for the Identification of Retinal Cells and Subcellular Organelles by Immunofluorescence Microscopy  (Laurie L. Molday, Christiana L. Cheng, Robert S. Molday)....Pages 293-310
 Immuno-TEM/STEM in Retinal Research  (Sanae Sakami, Krzysztof Palczewski)....Pages 311-332
 Noninvasive Two-Photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium  (Grazyna Palczewska, Timothy S. Kern, Krzysztof Palczewski)....Pages 333-343
 Analysis of the Drosophila Compound Eye with Light and Electron Microscopy  (Monalisa Mishra, Elisabeth Knust)....Pages 345-364
Front Matter ....Pages 365-365
 Cell-Based Therapy for Retinal Disease: The New Frontier  (Marco Zarbin)....Pages 367-381
 In Vitro Evaluation of AAV Vectors for Retinal Gene Therapy  (Johanna E. Wagner, Christian Schön, Elvir Becirovic, Martin Biel, Stylianos Michalakis)....Pages 383-390
 Nanoparticles Targeting Retinal and Choroidal Capillaries In Vivo  (Alexandra Haunberger, Achim Goepferich)....Pages 391-404
 Optimized Subretinal Injection Technique for Gene Therapy Approaches  (Regine Mühlfriedel, Stylianos Michalakis, Marina Garcia Garrido, Vithiyanjali Sothilingam, Christian Schön, Martin Biel et al.)....Pages 405-412
Back Matter ....Pages 413-417