Research Methods in Neurochemistry: Volume 2

Front Matter....Pages i-xviii
Front Matter....Pages 1-1
Bulk Separation of Neuronal Cell Bodies and Glial Cells in the Absence of Added Digestive Enzymes....Pages 3-38
Isolation of Brain Mitochondria....Pages 39-52
Preparation of Brain Mitochondrial Membranes....Pages 53-77
Isolation and Study of Synaptic Vesicles....Pages 79-98
Front Matter....Pages 99-99
Culture of Neural Tissue and Cells....Pages 101-160
Measurement of Arteriovenous Differences Across Rat Brain....Pages 161-169
Brain Blowing: A Technique for In Vivo Study of Brain Metabolism....Pages 171-182
Determination of Transport Rates In Vivo ....Pages 183-216
Front Matter....Pages 217-217
Isolation of Myelin Basic Proteins....Pages 219-245
Methods of Isolation and Identification of Neurophysin Proteins....Pages 247-279
Methods for the Neurochemical Study of Microtubules....Pages 281-300
Determination of Glycogen in Nervous Tissue....Pages 301-318
Front Matter....Pages 319-319
Methods for the Isolation and Determination of Glutamate, Glutamine, Aspartate, and γ-Aminobutyrate in Brain....Pages 321-359
Isolation and Determination of N -Acetyl- l -Aspartic Acid and Related Compounds in Brain....Pages 361-376
Methods for Analyzing Monoamine Oxidase and Catechol- O -methyltransferase in Nervous Tissue....Pages 377-388
The Pineal Organ....Pages 389-407
Back Matter....Pages 409-414