Reconstituting the Cytoskeleton

Content: 
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xiii-xviii

Preface: The Role of Reconstitution in Cytoskeleton Research
Pages xix-xxiii
Ronald D. Vale

Chapter One - Actin Filament Dynamics Using Microfluidics
Pages 3-17
Marie-France Carlier, Guillaume Romet-Lemonne, Antoine Jégou

Chapter Two - Bacterial Actin-Like Proteins: Purification and Characterization of Self-Assembly Properties
Pages 19-34
Natalie A. Petek, R. Dyche Mullins

Chapter Three - Quantitative Analysis of Microtubule Self-assembly Kinetics and Tip Structure
Pages 35-52
Louis S. Prahl, Brian T. Castle, Melissa K. Gardner, David J. Odde

Chapter Four - Biochemical Reconstitution of the WAVE Regulatory Complex
Pages 55-72
Baoyu Chen, Shae B. Padrick, Lisa Henry, Michael K. Rosen

Chapter Five - Rotational Movement of Formins Evaluated by Using Single-Molecule Fluorescence Polarization
Pages 73-94
Hiroaki Mizuno, Naoki Watanabe

Chapter Six - Single-Molecule Studies of Actin Assembly and Disassembly Factors
Pages 95-117
Benjamin A. Smith, Jeff Gelles, Bruce L. Goode

Chapter Seven - Assaying Microtubule Nucleation by the γ-Tubulin Ring Complex
Pages 119-130
Yuk-Kwan Choi, Robert Z. Qi

Chapter Eight - Reconstituting Dynamic Microtubule Polymerization Regulation by TOG Domain Proteins
Pages 131-148
Jawdat Al-Bassam

Chapter Nine - Generation of Differentially Modified Microtubules Using In Vitro Enzymatic Approaches
Pages 149-166
Annapurna Vemu, Christopher P. Garnham, Duck-Yeon Lee, Antonina Roll-Mecak

Chapter Ten - Engineering Defined Motor Ensembles with DNA Origami
Pages 169-188
Brian S. Goodman, Samara L. Reck-Peterson

Chapter Eleven - Construction and Analyses of Elastically Coupled Multiple-Motor Systems
Pages 189-204
Arthur Rogers, Pamela E. Constantinou, D. Kenneth Jamison, Jonathan W. Driver, Michael R. Diehl

Chapter Twelve - Reconstitution of Cortical Dynein Function
Pages 205-230
Sophie Roth, Liedewij Laan, Marileen Dogterom

Chapter Thirteen - Reconstitution of Microtubule-Dependent Organelle Transport
Pages 231-248
Pradeep Barak, Ashim Rai, Alok Kumar Dubey, Priyanka Rai, Roop Mallik

Chapter Fourteen - Reconstituting the Motility of Isolated Intracellular Cargoes
Pages 249-262
Adam G. Hendricks, Yale E. Goldman, Erika L.F. Holzbaur

Chapter Fifteen - Reconstitution of Contractile Actomyosin Arrays
Pages 265-282
Michael Murrell, Todd Thoresen, Margaret Gardel

Chapter Sixteen - Directed Actin Assembly and Motility
Pages 283-300
Rajaa Boujemaa-Paterski, Rémi Galland, Cristian Suarez, Christophe Guérin, Manuel Théry, Laurent Blanchoin

Chapter Seventeen - In Vitro Reconstitution of Dynamic Microtubules Interacting with Actin Filament Networks
Pages 301-320
Magdalena Preciado López, Florian Huber, Ilya Grigoriev, Michel O. Steinmetz, Anna Akhmanova, Marileen Dogterom, Gijsje H. Koenderink

Chapter Eighteen - Measuring Kinetochore–Microtubule Interaction In Vitro
Pages 321-337
Jonathan W. Driver, Andrew F. Powers, Krishna K. Sarangapani, Sue Biggins, Charles L. Asbury

Chapter Nineteen - Micropattern-Guided Assembly of Overlapping Pairs of Dynamic Microtubules
Pages 339-360
Franck J. Fourniol, Tai-De Li, Peter Bieling, R. Dyche Mullins, Daniel A. Fletcher, Thomas Surrey

Chapter Twenty - WAVE Regulatory Complex Activation
Pages 363-379
Peter J. Hume, Daniel Humphreys, Vassilis Koronakis

Chapter Twenty-One - Dissecting Principles Governing Actin Assembly Using Yeast Extracts
Pages 381-397
Alphée Michelot, David G. Drubin

Chapter Twenty-Two - Xenopus Egg Cytoplasm with Intact Actin
Pages 399-415
Christine M. Field, Phuong A. Nguyen, Keisuke Ishihara, Aaron C. Groen, Timothy J. Mitchison

Chapter Twenty-Three - Glycogen-Supplemented Mitotic Cytosol for Analyzing Xenopus Egg Microtubule Organization
Pages 417-433
Aaron C. Groen, Phuong A. Ngyuen, Christine M. Field, Keisuke Ishihara, Timothy J. Mitchison

Chapter Twenty-Four - Spindle Assembly on Immobilized Chromatin Micropatterns
Pages 435-448
Céline Pugieux, Serge Dmitrieff, Katarzyna Tarnawska, François Nédélec

Author Index
Pages 449-470

Subject Index
Pages 471-484