Recombinant DNA Laboratory Manual

Content: 
Front Matter, Page iii
Copyright, Page iv
Dedication, Page v
PREFACE, Pages xi-xii
SCHEDULE OF LABORATORY EXERCISES, Pages xiii-xiv
LAB I - BACTERIAL GROWTH PARAMETERS, Pages 1-10
LAB II - ISOLATION AND ANALYSIS OF BACTERIAL AND DROSOPHILA CHROMOSOMAL DNA, Pages 11-30
LAB III - PLASMID DNA ISOLATION AND AGAROSE GEL ANALYSIS, Pages 31-42
Lab IV - INTRODUCTION OF DNA INTO CELLS, Pages 43-49
LAB V - TN5 MUTAGENESIS OF PBR329, Pages 51-65
LAB VI - DNA CLONING IN M13, Pages 67-96
LAB VII - DNA SEQUENCING, Pages 97-115
LAB VIII - DNA GEL BLOTTING, PROBE PREPARATION, HYBRIDIZATION, AND HYBRID DETECTION, Pages 117-134
LAB IX - LAMBDA PHAGE MANIPULATIONS, Pages 135-149
Appendix 1 - USE OF COMPUTERS IN A MOLECULAR BIOLOGY LABORATORY, Pages 151-158
Appendix 2 - SAFETY IN THE RECOMBINANT DNA LABORATORY, Pages 159-163
Appendix 3 - SOLUTIONS, Pages 165-167
Appendix 4 - STRAINS AND DNA, Page 169
Appendix 5 - MOLECULAR BIOLOGY REAGENT SUPPLIERS, Pages 171-172
Appendix 6 - FREQUENTLY USED ENZYMES, Pages 173-174
Appendix 7 - FREQUENTLY USED TECHNIQUES NOT DESCRIBED IN LABS, Pages 175-190
INDEX, Pages 191-195