Recognition of Carbohydrates in Biological Systems, Part A: General Procedures

Content: 
Editors In Chief
Page ii

Contributors
Pages ix-xiv

Preface
Page xv

Methods In Enzymology
Pages xvii-xxxviii

Transition Metal-Catalyzed Cross-Coupling Reactions toward the Synthesis of О±-d-Mannopyranoside Clusters Original Research Article
Pages 3-17
Sanjoy K Das, M.Corazon Trono, RenГ© Roy

Ruthenium Carbenoids as Catalysts for Olefin Metathesis of П‰-Alkenyl Glycosides Original Research Article
Pages 17-28
Romyr Dominique, Sanjoy K Das, Bingcan Liu, Joe Nahra, Brad Schmor, Zhonghong Gan, RenГ© Roy

Digoxin Derivatives as Tools for Glycobiology Original Research Article
Pages 29-37
Gordan Lauc, Sandra Šupraha, Yuan C Lee, Mirna Flögel

Synthesis of Peptide-Based Trivalent Scaffold for Preparation of Cluster Glycosides Original Research Article
Pages 38-43
Reiko T Lee, Yuan C Lee

Chemoenzymatic Synthesis of Diverse Asparagine-Linked Oligosaccharides Original Research Article
Pages 44-64
Yasuhiro Kajihara, Yasuhiro Suzuki, Ken Sasaki, Lekh Raj Juneja

Enzymatic Synthesis of Neoglycoconjugates by Transglycosylation with Endo-ОІ-N-acetylglucosaminidase A Original Research Article
Pages 64-74
Kaoru Takegawa, Jian-Qiang Fan

Chemoenzymatic Synthesis of Neoglycopeptides Using Endo ОІ-N-acetylglucosaminidase from Mucor hiemalis Original Research Article
Pages 74-85
Katsuji Haneda, Toshiyuki Inazu, Mamoru Mizuno, Kenji Yamamoto

Preparative-Scale Chemoenzymatic Synthesis of Large Carbohydrate Assemblies Using α(1→4)-Galactosyltransferase⧸UDP-4′-Gal-Epimerase Fusion Protein Original Research Article
Pages 86-105
Pavel I Kitov, Eugenia Paszkiewicz, Warren W Wakarchuk, David R Bundle

Synthesis of Galactose-Containing Oligosaccharides through Superbeads and Superbug Approaches: Substrate Recognition along Different Biosynthetic Pathways Original Research Article
Pages 106-124
Jianbo Zhang, Xi Chen, Jun Shao, Ziye Liu, Przemyslaw Kowal, Yuquan Lu, Peng G Wang

Conjugating Low Molecular Mass Carbohydrates to Proteins 1. Monitoring the Progress of Conjugation Original Research Article
Pages 125-139
Rina Saksena, Anatoly Chernyak, Alex Karavanov, Pavol KovГЎДЌ

Conjugating Low Molecular Mass Carbohydrates to Proteins 2. Recovery of Excess Ligand Used in the Conjugation Reaction Original Research Article
Pages 140-159
Rina Saksena, Anatoly Chernyak, Emmanuel Poirot, Pavol KovГЎДЌ

Neoglycolipid Technology: Deciphering Information Content of Glycome Original Research Article
Pages 160-195
Wengang Chai, Mark S. Stoll, Christine Galustian, Alexander M. Lawson, Ten Feizi

Preparation and Utility of Neoproteoglycan Probes in Analyses of Interaction with Glycosaminoglycan-Binding Proteins Original Research Article
Pages 196-209
Haruko Ogawa, Haruko Ueda, Ayumi Natsume, Risa Suzuki

Modular Synthesis and Study of Multivalent Carbohydrate Ligands with Long and Flexible Linkers Original Research Article
Pages 209-218
Zhongsheng Zhang, Erkang Fan

Sugar Arrays in Microtiter Plates Original Research Article
Pages 218-225
Chi-Huey Wong, Marian C Bryan

Comparative Studies of Galactoside-Containing Clusters Original Research Article
Pages 226-239
Bingcan Liu, RenГ© Roy

Multivalent Breast Cancer T-Antigen Markers Scaffolded onto PAMAM Dendrimers Original Research Article
Pages 240-249
RenГ© Roy, Myung-Gi Baek

Constructing Azide-Labeled Cell Surfaces Using Polysaccharide Biosynthetic Pathways Original Research Article
Pages 249-272
Sarah J Luchansky, Howard C Hang, Eliana Saxon, Jocelyn R Grunwell, Chong Yu, Danielle H Dube, Carolyn R Bertozzi

Cell Wall Engineering of Living Bacteria through Biosynthesis Original Research Article
Pages 273-286
Reiko Sadamoto, Kenichi Niikura, Kenji Monde, Shin-Ichiro Nishimura

Molecular Biological Fingerprinting of Human Lectin Expression by RT-PCR Original Research Article
Pages 287-297
Harald Lahm, Sabine AndrГ©, Andreas Hoeflich, JГјrgen R. Fischer, Bernard Sordat, Herbert Kaltner, Eckhard Wolf, Hans-Joachim Gabius

Visualization and Characterization of Receptor Clusters by Transmission Electron Microscopy Original Research Article
Pages 301-312
Jason E Gestwicki, Christopher W Cairo, M.Jack Borrok, Laura L Kiessling

Exploring Kinetics and Mechanism of Protein–Sugar Recognition by Surface Plasmon Resonance Original Research Article
Pages 312-329
Mili Kapoor, Celestine J Thomas, Kiran Bachhawat-Sikder, Shilpi Sharma, Avadhesha Surolia

Quantitative Lectin–Carbohydrate Interaction Analysis on Solid-Phase Surfaces Using Biosensor Based on Surface Plasmon Resonance Original Research Article
Pages 330-340
Hiroyuki Sota, Reiko T. Lee, Yuan C. Lee, Yasuro Shinohara

Surface Plasmon Resonance Study of RNA–Aminoglycoside Interactions Original Research Article
Pages 340-353
Chi-Huey Wong, Fu-Sen Liang

Frontal Affinity Chromatography as a Tool for Elucidation of Sugar Recognition Properties of Lectins Original Research Article
Pages 353-368
Jun Hirabayashi, Yoichiro Arata, Ken-ichi Kasai

Evaluating Carbohydrate–Protein Binding Interactions Using Frontal Affinity Chromatography Coupled to Mass Spectrometry Original Research Article
Pages 369-376
Monica M Palcic, Boyan Zhang, Xiangping Qian, Brian Rempel, Ole Hindsgaul

Determination of Protein–Oligosaccharide Binding by Nanoelectrospray Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry Original Research Article
Pages 376-397
Weijie Wang, Elena N. Kitova, John S. Klassen

Analysis of Lectin–Carbohydrate Interactions by Capillary Affinophoresis Original Research Article
Pages 398-417
Kiyohito Shimura, Ken-ichi Kasai

Describing Topology of Bound Ligand by Transferred Nuclear Overhauser Effect Spectroscopy and Molecular Modeling Original Research Article
Pages 417-434
Hans-Christian Siebert, JesГєs JimГ©nez-Barbero, Sabine AndrГ©, Herbert Kaltner, Hans-Joachim Gabius

Studies of Carbohydrate–Protein Interaction by Capillary Electrophoresis Original Research Article
Pages 434-454
Susumu Honda, Atsushi Taga

Carbohydrate–Lectin Cross-Linking Interactions: Structural, Thermodynamic, and Biological Studies Original Research Article
Pages 455-486
Tarun K Dam, C.Fred Brewer

Calorimetric Evaluation of Protein–Carbohydrate Affinities Original Research Article
Pages 486-504
Trine Christensen, Eric J Toone

Fluorescence Polarization to Study Galectin–Ligand Interactions Original Research Article
Pages 504-512
Pernilla Sörme, Barbro Kahl-Knutson, Ulf Wellmar, Ulf J Nilsson, Hakon Leffler

Lectin from Bulbs of Crocus sativus Recognizing N-Linked Core Glycan: Isolation and Binding Studies Using Fluorescence Polarization Original Research Article
Pages 512-522
Kazuaki Kakehi, Mitsuhiro Kinoshita, Yasuo Oda, Badrulhisam Abdul-Rahman

Direct Fluorescence-Based Detection Methods for Multivalent Interactions Original Research Article
Pages 523-542
Xuedong Song, Jeane Shi, Basil I Swanson

Ultrasensitive Analysis of Sialic Acids and Oligo⧸Polysialic Acids by Fluorometric High-Performance Liquid Chromatography Original Research Article
Pages 543-560
Sadako Inoue, Yasuo Inoue

Identification of Lectins from Genomic Sequence Data Original Research Article
Pages 560-567
Kurt Drickamer, Maureen E Taylor

Exploring Enzyme Amplification to Characterize Specificities of Protein–Carbohydrate Recognition Original Research Article
Pages 567-583
Anita Ramdas Patil, Sandra Misquith, Tarun Kanti Dam, Vivek Sharma, Mili Kapoor, Avadhesha Surolia

Author Index
Pages 585-612

Subject Index
Pages 613-625