Receptor-Receptor Interactions in the Central Nervous System

Front Matter ....Pages i-xix
 Analysis and Quantification of GPCR Allosteric Receptor–Receptor Interactions Using Radioligand Binding Assays: The A2AR-D2R Heteroreceptor Complex Example  (Dasiel O. Borroto-Escuela, Miguel Pérez de la Mora, Michele Zoli, Fabio Benfenati, Manuel Narvaez, Alicia Rivera et al.)....Pages 1-14
 Analysis and Quantification of GPCR Heteroreceptor Complexes and Their Allosteric Receptor–Receptor Interactions Using Radioligand Binding Autoradiography  (Manuel Narvaez, Fidel Corrales, Ismel Brito, Ismael Valladolid-Acebes, Kjell Fuxe, Dasiel O. Borroto-Escuela)....Pages 15-23
 On the Study of D4R-MOR Receptor–Receptor Interaction in the Rat Caudate Putamen: Relevance on Morphine Addiction  (Alicia Rivera, Alejandra Valderrama-Carvajal, Diana Suárez-Boomgaard, Kirill Shumilov, M. Ángeles Real, Kjell Fuxe et al.)....Pages 25-39
 Use of Superfused Synaptosomes to Understand the Role of Receptor–Receptor Interactions as Integrative Mechanisms in Nerve Terminals from Selected Brain Region  (Sarah Beggiato, Sergio Tanganelli, Tiziana Antonelli, Maria Cristina Tomasini, Kjell Fuxe, Dasiel O. Borroto-Escuela et al.)....Pages 41-55
 Detection of Fibroblast Growth Factor Receptor 1 (FGFR1) Transactivation by Muscarinic Acetylcholine Receptors (mAChRs) in Primary Neuronal Hippocampal Cultures Through Use of Biochemical and Morphological Approaches  (Valentina Di Liberto, Giuseppa Mudó, Dasiel O. Borroto-Escuela, Kjell Fuxe, Natale Belluardo)....Pages 57-70
 Electrophysiological Approach to GPCR–RTK Interaction Study in Hippocampus of Adult Rats  (Davide Lattanzi, David Savelli, Michael Di Palma, Stefano Sartini, Silvia Eusebi, Dasiel O. Borroto-Escuela et al.)....Pages 71-90
 In Vivo Microdialysis Technique Applications to Understand the Contribution of Receptor–Receptor Interactions to the Central Nervous System Signaling  (Sergio Tanganelli, Tiziana Antonelli, Sarah Beggiato, Maria Cristina Tomasini, Kjell Fuxe, Dasiel O. Borroto-Escuela et al.)....Pages 91-107
 Behavioral Methods to Study the Impact of Receptor–Receptor Interactions in Fear and Anxiety  (Miguel Pérez de la Mora, José del Carmen Rejón-Orantes, Minerva Crespo-Ramírez, Dasiel O. Borroto-Escuela, Kjell Fuxe)....Pages 109-131
 Small Interference RNA Knockdown Rats in Behavioral Functions: GALR1/GALR2 Heteroreceptor in Anxiety and Depression-Like Behavior  (Antonio Flores-Burgess, Carmelo Millón, Belén Gago, José Angel Narváez, Kjell Fuxe, Zaida Díaz-Cabiale)....Pages 133-148
 Double Fluorescent Knock-In Mice to Investigate Endogenous Mu-Delta Opioid Heteromer Subcellular Distribution  (Lyes Derouiche, Stéphane Ory, Dominique Massotte)....Pages 149-162
 Biochemical Characterization of Dopamine D2 Receptor-Associated Protein Complexes Using Co-Immunoprecipitation and Protein Affinity Purification Assays  (Ping Su, Frankie H. F. Lee, Fang Liu)....Pages 163-186
 Methods to Identify the Signature of Trimers Formed by Three G Protein-Coupled Receptors or by Two G Protein-Coupled and One Ionotropic Receptor with Special Emphasis in the Functional Role in the Central Nervous System  (Irene Reyes-Resina, Eva Martínez-Pinilla, Dasiel O. Borroto-Escuela, Kjell Fuxe, Gemma Navarro, Rafael Franco)....Pages 187-203
 Bimolecular Fluorescence Complementation Methodology to Study G Protein-Coupled Receptor Dimerization in Living Cells  (Doungkamol Alongkronrusmee, Val J. Watts, Richard M. van Rijn)....Pages 205-221
 Detection and Quantitative Analysis of Dynamic GPCRs Interactions Using Flow Cytometry-Based FRET  (Barbara Chruścicka, Shauna E. Wallace Fitzsimons, Clémentine M. Druelle, Timothy G. Dinan, Harriët Schellekens)....Pages 223-238
 Assessing GPCR Dimerization in Living Cells: Comparison of the NanoBiT Assay with Related Bioluminescence- and Fluorescence-Based Approaches  (Elise Wouters, Lakshmi Vasudevan, Francisco Ciruela, Deepak K. Saini, Christophe Stove, Kathleen Van Craenenbroeck)....Pages 239-250
 Proximity Biotinylation for Studying G Protein-Coupled Receptor Dimerization  (Maxwell S. DeNies, Luciana K. Rosselli-Murai, Victoria L. Murray, Elisabeth M. Steel, Allen P. Liu)....Pages 251-263
 Conformational Profiling of the 5-HT2A Receptor Using FlAsH BRET  (Pavel Powlowski, Kyla Bourque, Jace Jones-Tabah, Rory Sleno, Dominic Devost, Terence E. Hébert)....Pages 265-282
 Searching the GPCR Heterodimer Network (GPCR-hetnet) Database for Information to Deduce the Receptor–Receptor Interface and Its Role in the Integration of Receptor Heterodimer Functions  (Ismel Brito, Manuel Narvaez, David Savelli, Kirill Shumilov, Michael Di Palma, Stefano Sartini et al.)....Pages 283-298
 Detection, Analysis, and Quantification of GPCR Homo- and Heteroreceptor Complexes in Specific Neuronal Cell Populations Using the In Situ Proximity Ligation Assay  (Dasiel O. Borroto-Escuela, Manuel Narvaez, Ismael Valladolid-Acebes, Kirill Shumilov, Michael Di Palma, Karolina Wydra et al.)....Pages 299-315
 Unraveling the Functions of Endogenous Receptor Oligomers in the Brain Using Interfering Peptide: The Example of D1R/NMDAR Heteromers  (Andry Andrianarivelo, Estefani Saint-Jour, Pierre Trifilieff, Peter Vanhoutte)....Pages 317-328
 Super-Resolution Imaging as a Method to Study GPCR Dimers and Higher-Order Oligomers  (Kim C. Jonas, Aylin C. Hanyaloglu)....Pages 329-343
Back Matter ....Pages 345-346