Rapid Cycle Real-Time PCR — Methods and Applications: Genetics and Oncology

Front Matter....Pages I-VIII
Introduction for Genetics and Oncology Volume Rapid Cycle Real-Time PCR Methods and Applications ....Pages 1-2
Front Matter....Pages 3-3
Quantification of Cytokine mRNAs in Human Myocardial Biopsy Samples by Real-Time Quantitative PCR Technology Using the LightCycler Instrument....Pages 5-14
Quantitative Two-Step RT-PCR for the Detection of Human ABCA1 Transporter on LightCycler Using Hybridization Probes and External Standards....Pages 15-25
Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene....Pages 27-33
Genotyping by Guanosine-Dependent Quenching of Single-Labeled Fluorescein Probes....Pages 35-46
Limitations of Melting Curve Analysis Using SYBR Green I — Fragment Differentiation and Mutation Detection in the CFTR-Gene....Pages 47-56
SYBR Green I Analysis of the Trinucleotide Repeat Responsible for Huntington’s Disease....Pages 57-63
Front Matter....Pages 65-65
Parallel Genotyping of Different Genes: A Rapid Real-Time PCR Approach....Pages 67-75
Detection of a Single Base Substitution in Single Cells by Melting Peak Analysis Using Dual-Color Hybridization Probes....Pages 77-84
Rapid Screening for Five Major Cystic Fibrosis Mutations by Melting Peak Analysis Using Fluorogenic Hybridization Probes....Pages 85-94
LightCycler PCR for the Polymorphisms -308 and -238 in the TNF Alpha Gene and for the TNFB1/B2 Polymorphism in the LT Alpha Gene....Pages 95-105
Rapid Genotyping of 2-bp and 9-bp Deletion Mutations Using the LightCycler Instrument....Pages 107-114
Genotyping of the Methionine-Valine Polymorphism at Codon 129 of the Human Prion Protein by Melting Point Analysis of Fluorescently Labeled Hybridization Probes....Pages 115-127
Rapid Detection of Missense Mutations in the Prostatic Steroid 5α-Reductase Gene Using Real-Time Fluorescence PCR and Melting Curve Analysis....Pages 129-135
Front Matter....Pages 137-137
Analysis of Microsatellite Instability by Melting Peak Analysis with BAT26 and BAT25 Specific Fluorescence Hybridization Probes....Pages 139-146
Two Color Multiplexing and Typing of Human Papillomavirus Types 16,18 and 45 on LightCycler....Pages 147-157
Quantitative Analysis of AML1-ETO Fusion Transcripts in t(8;21) Positive AML Using Real-Time RT-PCR....Pages 159-168
Rapid Quantitative Detection of Free Cancer Cells in the Peritoneal Cavity of Gastric Cancer Patients with Real-Time CEA RT-PCR Using Hybridization Probes....Pages 169-175
Quantitative Measurement of the mRNA Expression of the Tumor-Associated Antigen PRAME by Real-Time RT-PCR Using LightCycler and SYBR Green I Technology....Pages 177-186
Expression Analysis of Telomerase-Genes hTERT and hTR by Quantitative PCR on LightCycler....Pages 187-197
Front Matter....Pages 137-137
Measurement of MDR1 Gene Expression by Real-Time Quantitative RT-PCR Using the LightCycler Instrument....Pages 199-205