Rapid Cycle Real-Time PCR — Methods and Applications: Quantification

Front Matter....Pages I-VII
Front Matter....Pages 1-1
Housekeeping Genes: A Gold Standard?....Pages 3-10
The Choice of House Keeping Genes in MRD-Quantification of AML1-ETO Positive Acute Myeloid Leukemia....Pages 11-20
Quantification of mRNA Using Linear Regression of Log-Linear PCR Data-Points as an Alternative for the Standard Curve Approach....Pages 21-29
Measuring Genome Sizes by Absolute Quantification....Pages 31-41
Front Matter....Pages 43-43
Relative Quantification of Insulin Gene Expression on the LightCycler Using SYBR Green I....Pages 45-51
Quantification of δRec-ψJα Signal Joint T-Cell Receptor Excision Circle DNA in Patients after Autologous and Allogeneic Stem Cell Transplantation....Pages 53-59
Expression Analysis of Mitochondrial Components in a Variety of Plant Species Using Real-Time Quantitative PCR....Pages 61-72
Quantification of Ikaros Splice Variants by Real-Time PCR....Pages 73-82
Methods to Quantify Cytokine Gene Expression by Real-Time PCR....Pages 83-92
Profiling Breast Cancer Using Real-Time Quantitative PCR....Pages 95-106
 HER-2/neu Gene Copy Number Quantified by Real-Time PCR in Cell Lines and Breast Cancer Tissue....Pages 107-116
Quantification of Several Components of Fibrinolytic and Matrix Metalloproteinase Systems in Primary Breast Cancer....Pages 117-124
A Rapid Cycle Real-Time Quantitative β-globin PCR for Quantification of Human DNA in Feces....Pages 125-130
Quantification of Tumor Load for Follicular Lymphoma by Quantifying BCL2/IGH Using Real-Time Quantitative PCR by LightCycler™....Pages 131-140
Real-Time Quantification of the AML Rearrangements, AML1-ETO and TEL-AML1 , in Acute Leukemia....Pages 141-155
Rapid Detection of Gene Duplications in Charcot-Marie-Tooth 1A Disease by SNP Genotyping Using Real-Time PCR....Pages 159-169
Cytochrome P450 2D6 Deletion Genotyping Using Derivative Curve Analysis on the LightCycler....Pages 171-178
Trisomy 21 Detected by SNP Allele Ratios....Pages 179-186
Quantitative Chimerism Analysis by Allele-Specific Real-Time PCR of a 10 bp Insertion/Deletion Polymorphism within the Promotor Region of Factor Vllc....Pages 187-195
Detection of SARS-Coronavirus in the LightCycler by 5′-Nuclease Real-Time RT-PCR....Pages 199-210
Front Matter....Pages 43-43
Normalized Quantitative Rapid-Cycle Real-Time PCR for the Assessment of CMV-DNA Copies....Pages 211-223