Quantitative Applications of Mass Spectrometry

Quantitative Applications of Mass Spectrometry......Page 4
Contents......Page 10
Preface......Page 12
Acknowledgements......Page 14
Introduction......Page 16
1.1 Ion Sources......Page 18
1.1.1 Electron Ionization......Page 20
1.1.2 Chemical Ionization......Page 21
1.1.3 Atmospheric Pressure Chemical Ionization......Page 23
1.1.4 Electrospray Ionization......Page 25
1.1.5 Atmospheric Pressure Photoionization......Page 28
1.1.6 Matrix-assisted Laser Desorption/Ionization......Page 29
1.2.1 Mass Resolution......Page 31
1.2.2 Sector Analysers......Page 32
1.2.3 Quadrupole Analysers......Page 36
1.2.4 Time-of-flight......Page 42
1.3.1 Total Ion Current (TIC) Chromatogram......Page 44
1.3.2 Reconstructed Ion Chromatogram (RIC)......Page 45
1.4 LC/MS......Page 46
1.5.1 MS/MS by Double Focusing Instruments......Page 47
1.5.2 MS/MS by Triple Quadrupoles......Page 48
1.5.3 MS/MS by Ion Traps......Page 49
References......Page 51
2 How to Design a Quantitative Analysis......Page 54
2.1 General Strategy......Page 55
2.1.2 Sampling......Page 58
2.1.3 Sample Treatment......Page 59
2.1.4 Instrumental Analysis......Page 60
References......Page 70
3 How to Improve Specificity......Page 72
3.1.1 GC/MS Measurements in Low and High Resolution Conditions......Page 73
3.1.2 LC/ESI/MS and LC/APCI/MS Measurements......Page 78
3.2 Choice of a Suitable Ionization Method......Page 96
3.3.1 Use of High Resolution MID Analysis......Page 102
3.3.2 NICI in the Analysis of Dioxins, Furans and PCBs......Page 110
3.3.3 MS/MS in the Detection of Dioxins, Furans and PCBs......Page 112
3.4 An Example of MALDI/MS in Quantitative Analysis of Polypeptides: Substance P......Page 118
References......Page 123
4 Some Thoughts on Calibration and Data Analysis......Page 124
4.2 Homoscedastic and Heteroscedastic Data......Page 125
4.3.1 Unweighted Regression......Page 126
4.3.2 Weighted Regression......Page 136
4.3.3 A Practical Example......Page 143
4.4 Different Approaches to Estimate Detection and Quantification Limits......Page 147
References......Page 149
Index......Page 152