Purines in Cellular Signaling: Targets for New Drugs

Front Matter....Pages i-xv
Front Matter....Pages 1-1
Adenosine Agonists and Antagonists....Pages 3-12
Intracellular and Extracellular Metabolism of Adenosine and Adenine Nucleotides....Pages 13-19
Adenosine Deaminase and Adenosine Transport Systems in the CNS....Pages 20-25
Transport Systems for Adenosine in Mammalian Cell Membranes....Pages 26-32
Formation of Adenosine in the Heart from Extracellular Adenine Nucleotides....Pages 33-40
Central Nervous System Effects of Adenosine....Pages 41-47
Adenosine Receptor Sub-types in Isolated Tissues: Antagonist Studies....Pages 48-53
Molecular Probes for Adenosine Receptors....Pages 54-64
Purification of A 1 Adenosine Receptor of Rat Brain Membranes by Affinity Chromatography....Pages 65-68
Characteristics of A 1 Adenosine Receptors and Guanine Nucleotide Binding Proteins Co-Purified from Bovine Brain....Pages 69-72
Antagonist Radioligand Binding to Solubilized Porcine Atrial A 1 Adenosine Receptors....Pages 73-77
Isolation of an Adenosine Binding Protein Which Has Properties Expected of the A 1 Adenosine Receptor....Pages 78-81
Identification of the A 2 Adenosine Receptor Binding Subunit by Photoaffinity Crosslinking....Pages 82-85
Human Placental Adenosine A 2 -Like Binding Sites Properties and Homology with Mammalian and Avian Stress Proteins....Pages 86-89
Cardiac Interstitial Fluid Adenosine in Normoxia and Hypoxia....Pages 90-95
Rationale for the Use of Adenosine in the Diagnosis and Treatment of Cardiac Arrhythmias....Pages 95-99
The Central Adenosine System as a Therapeutic Target in Stroke....Pages 100-107
Adenosine-Mediated Vasoconstriction in the Skin Microcirculation....Pages 108-113
Adenosine is an Antiinflammatory Autocoid: Adenosine Receptor Occupancy Promotes Neutrophil Chemotaxis and Inhibits Superoxide Anion Generation....Pages 114-119
Beta Adrenergic Receptor Mediated Stimulation of Adenine Nucleotide Catabolism and Purine Release in Human Adipocytes....Pages 120-125
Front Matter....Pages 1-1
Structure Activity Relationships for Adenosine Antagonists....Pages 126-135
Structure Activity Relationships for Adenosine Agonists....Pages 136-145
The Design of a Series of Highly A 2 Selective Adenosine Agonists....Pages 146-151
Adenosine Agonists. Characterization of the N 6 -Subregion of the Adenosine A 2 Receptor via Molecular Modeling Techniques....Pages 152-157
Physiological and Biochemical Aspects of “P”-Site-Mediated Inhibition of Adenylyl Cyclase....Pages 158-165
Adenosine Receptors Mediating Inhibition of Peripheral and Central Neurons....Pages 166-173
Adenosine Receptors as Drug Targets: Fulfilling the Promise?....Pages 174-183
Mechanisms of Adenosine Action....Pages 184-193
Functional Roles of A 1 and A 2 Receptors in Rat Hippocampus and Striatum....Pages 194-199
Subclassification of Neuronal Adenosine Receptors....Pages 200-205
Effects of Adenosine on Mast Cells....Pages 206-212
The Role of Adenosine on the Gastric Acid Secretory Response....Pages 213-219
Adenosine Receptors and Signaling in the Kidney....Pages 220-225
Inhibition of the Function of Guanine Nucleotide Binding Proteins by the New Positive Inotropes....Pages 226-233
Blockade of Antigen Activated Signals by Xanthine Analogs in RBL-2H3 Cells: Evidence for a Novel Adenosine Receptor....Pages 233-238
Front Matter....Pages 239-239
Classification and Characterization of Purinoceptors....Pages 241-253
Structure Activity Relationships for Adenine Nucleotide Receptors on Mast Cells, Human Platelets, and Smooth Muscle....Pages 254-259
Modulation of Norepinephrine Release by ATP and Adenosine....Pages 260-265
The Phospholipase C Linked P 2y -Purinergic Receptor....Pages 266-271
Roles of an ADP Receptor on the Platelet Surface in Mediating Aggregation and Fibrinogen Binding....Pages 272-278
Front Matter....Pages 279-279
Structural and Functional Characterization of Cyclic GMP-Stimulated Phosphodiesterases and Their Role in Intracellular Signal Transduction....Pages 281-289
Phosphodiesterase Genes from Flies to Mammals....Pages 290-296
Structure and Function of the High Affinity, Rolipram and RO 2-1724 Sensitive, cAMP Phosphodiesterases....Pages 297-302
Correlation of 3 H-Rolipram Binding to a Cerebral cAMP Phosphodiesterase with Antidepressant Activity....Pages 303-308
Mechanism for Dual Regulation of the Particulate cGMP-Inhibited cAMP Phosphodiesterase in Rat Adipocytes by Isoproterenol and Insulin....Pages 309-314
Expression of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase in Developing and Adult Rat Brain....Pages 315-322
Regulation of cAMP Metabolism in PC12 Cells by Type II (cGMP-Activatable) Cyclic Nucleotide Phosphodiesterase....Pages 323-328
cGMP-Binding Phosphodiesterase: Novel Effects of cGMP Binding on the Interaction Between Functional Domains in the Enzyme....Pages 329-335
Clinical Investigations with Phosphodiesterase Inhibitors....Pages 336-346
Chemical Probes of Type IV Phosphodiesterase: Design, Synthesis, and Characterization of ( 3 H)-2Y186126 as a Radioligand for Sites Within Cardiac Membranes....Pages 347-352
Purification of a cGMP-inhibited cAMP Phosphodiesterase from Vascular Smooth Muscle....Pages 353-357
Low K m cAMP Phosphodiesterase Isozymes and Modulation of Tone in Vascular, Airway and Gastrointestinal Smooth Muscle....Pages 358-364
Structure Activity Relationships of Xanthines as Inhibitors of Cyclic Nucleotide Phosphodiesterases and as Antagonists of Adenosine Receptors....Pages 365-369
Mechanisms of Xanthine Actions in Models of Asthma....Pages 370-375
Back Matter....Pages 377-448