Protocols for Neural Cell Culture

Front Matter....Pages i-xx
Outgrowth Assays and Cortical Slice Cultures....Pages 1-12
Microexplant Cultures of the Cerebellum....Pages 13-23
Aggregating Neural Cell Cultures....Pages 25-49
Primary Cell Cultures of Peripheral and Central Neurons and Glia....Pages 51-75
Chick Spinal Somatic Motoneurons in Culture....Pages 77-89
Neural Cells in Microcultures....Pages 91-106
Construction and Use of Compartmented Cultures....Pages 107-116
Astrocyte and Oligodendrocyte Cultures....Pages 117-130
Generation of Mouse Astroglia and Microglia Cultures from Mouse Neopallium....Pages 131-141
Generation of Oligodendroblasts from Primary Cultures of Rat Neopallium....Pages 143-156
Culture of Glial Cells from Human Brain Biopsies....Pages 157-172
Colony Cultures....Pages 173-183
Herpes Simplex Virus Vectors for Gene Therapy of the Nervous System....Pages 185-195
Immunostaining and Identification of Antigens....Pages 197-205
Elimination of Cell Types from Mixed Neural Cell Cultures....Pages 207-217
Quantification of Cells in Culture....Pages 219-233
Procedures for Subculturing Cells....Pages 235-240
Preparation of Tissue-Cultured Material for Electron Microscopical Observation....Pages 241-251
Tips in Tissue Culture....Pages 253-261
Use of Dissecting Instruments in Tissue Culture....Pages 263-266
Back Matter....Pages 267-278