Proteus mirabilis: Methods and Protocols

Front Matter ....Pages i-x
 Proteus mirabilis Overview  (Harry L. T. Mobley)....Pages 1-4
 Culture Methods for Proteus mirabilis  (Melanie M. Pearson)....Pages 5-13
 Methods for Studying Swarming and Swimming Motility  (Melanie M. Pearson)....Pages 15-25
 Testing the Ability of Compounds to Induce Swarming  (Chelsie E. Armbruster)....Pages 27-34
 Purification of Native Flagellin  (María José González, Victoria Iribarnegaray, Pablo Zunino, Paola Scavone)....Pages 35-44
 Analysis of Proteus mirabilis Social Behaviors on Surfaces  (Kristin Little, Karine A. Gibbs)....Pages 45-59
 Insertional Mutagenesis Protocol for Constructing Single or Sequential Mutations  (Melanie M. Pearson, Stephanie D. Himpsl, Harry L. T. Mobley)....Pages 61-76
 Allelic Exchange Mutagenesis in Proteus mirabilis  (Kristen E. Howery, Philip N. Rather)....Pages 77-84
 Quantification of Urease Activity  (Shawn Richmond, Alejandra Yep)....Pages 85-96
 Siderophore Detection Using Chrome Azurol S and Cross-Feeding Assays  (Stephanie D. Himpsl, Harry L. T. Mobley)....Pages 97-108
 Using Hemagglutination, Surface Shearing, and Acid Treatment to Study Fimbriae in Proteus mirabilis  (Stephanie D. Himpsl, Melanie M. Pearson, Harry L. T. Mobley)....Pages 109-120
 Phase Variation of the mrp Fimbrial Promoter  (Melanie M. Pearson)....Pages 121-127
 Adherence of Proteus mirabilis to Uroepithelial Cells  (María José González, Victoria Iribarnegaray, Paola Scavone, Pablo Zunino)....Pages 129-137
 An In Vitro Bladder Model for Studying Catheter-Associated Urinary Tract Infection and Associated Analysis of Biofilms  (Jonathan Nzakizwanayo, Harriet Pelling, Scarlet Milo, Brian V. Jones)....Pages 139-158
 Independent Transurethral Urinary Tract Inoculation in a Murine Model of Ascending Infection with Proteus mirabilis  (Sara N. Smith)....Pages 159-172
 Cochallenge Inoculation with Proteus mirabilis in a Murine Transurethral Urinary Tract Model of Ascending Infection  (Sara N. Smith)....Pages 173-186
 Indwelling Urinary Catheter Model of Proteus mirabilis Infection  (Sara N. Smith, Chelsie E. Armbruster)....Pages 187-200
 Vaccination to Protect Against Proteus mirabilis Challenge Utilizing the Ascending Model of Urinary Tract Infection  (Sara N. Smith, Stephanie D. Himpsl, Harry L. T. Mobley)....Pages 201-215
 Characterization of Proteus mirabilis Lipopolysaccharide Samples by Infrared Spectroscopy and Serological Methods  (Katarzyna Durlik, Grzegorz Czerwonka, Paulina Żarnowiec, Wiesław Kaca)....Pages 217-230
 Isolation and Purification of Proteus mirabilis Bacteriophage  (Agnieszka Maszewska, Antoni Różalski)....Pages 231-240
 Detection of Neutrophil Extracellular Traps in Urine  (Yanbao Yu, Keehwan Kwon, Rembert Pieper)....Pages 241-257
 Using Proteomics to Identify Inflammation During Urinary Tract Infection  (Yanbao Yu, Rembert Pieper)....Pages 259-272
 Assessment of Proteus mirabilis Antigen Immunological Complexes by Atomic Force Microscopy  (Wiesław Kaca, Joanna Gleńska-Olender, Iwona Konieczna, Józef Gawęda, Sławomir Sęk)....Pages 273-283
 Considerations for Modeling Proteus mirabilis Swarming  (Bruce P. Ayati)....Pages 285-296
 Transposon Insertion Site Sequencing in a Urinary Tract Model  (Valerie S. Forsyth, Harry L. T. Mobley, Chelsie E. Armbruster)....Pages 297-337
Back Matter ....Pages 339-342