Proteomic Methods in Neuropsychiatric Research

Front Matter....Pages i-xii
Front Matter....Pages 1-1
Application of Proteomic Techniques for Improved Stratification and Treatment of Schizophrenia Patients....Pages 3-19
Multiplexing Biomarker Methods, Proteomics and Considerations for Alzheimer’s Disease....Pages 21-48
What Have Proteomic Studies Taught Us About Novel Drug Targets in Autism?....Pages 49-67
Application of Proteomic Approaches to Accelerate Drug Development for Psychiatric Disorders....Pages 69-84
Proteomic Biomarker Identification in Cerebrospinal Fluid for Leptomeningeal Metastases with Neurological Complications....Pages 85-96
Connecting Brain Proteomics with Behavioural Neuroscience in Translational Animal Models of Neuropsychiatric Disorders....Pages 97-114
LC-MSE for Qualitative and Quantitative Proteomic Studies of Psychiatric Disorders....Pages 115-129
The Utility of Multiplex Assays for Identification of Proteomic Signatures in Psychiatry....Pages 131-138
Front Matter....Pages 139-139
Blood Sampling and Preparation Procedures for Proteomic Biomarker Studies of Psychiatric Disorders....Pages 141-147
Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders....Pages 149-156
Sequential Immunoprecipitation of Secretory Vesicle Proteins from Biosynthetically Labelled Cells....Pages 157-165
2D Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically Labelled Pancreatic Islets....Pages 167-174
Two-Dimensional Gel Electrophoresis: A Reference Protocol....Pages 175-182
A Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Protocol for Studies of Neural Precursor Cells....Pages 183-191
Identifying Biomarker Candidates in the Blood Plasma or Serum Proteome....Pages 193-203
Selective Reaction Monitoring Mass Spectrometry for Quantitation of Glycolytic Enzymes in Postmortem Brain Samples....Pages 205-212
A Selected Reaction Monitoring Mass Spectrometry Protocol for Validation of Proteomic Biomarker Candidates in Studies of Psychiatric Disorders....Pages 213-218
Application of iTRAQ Shotgun Proteomics for Measurement of Brain Proteins in Studies of Psychiatric Disorders....Pages 219-227
Co-immunoprecipitation for Deciphering Protein Interactomes....Pages 229-236
Sequential Co-immunoprecipitation and Immunoblot Approach to Determine Oligomerisation of G-Protein-Coupled Receptors....Pages 237-243
Front Matter....Pages 139-139
A Clinical Study Protocol to Identify Serum Biomarkers Predictive of Response to Antipsychotics in Schizophrenia Patients....Pages 245-250
A Protocol for Producing the Maternal Low-Protein Rat Model: A Tool for Preclinical Proteomic Studies....Pages 251-255
Generation of the Acute Phencyclidine Rat Model for Proteomic Studies of Schizophrenia....Pages 257-261
A Protocol for Generation of a Corticosterone Model of Psychiatric Disorders....Pages 263-268
MK-801-Treated Oligodendrocytes as a Cellular Model to Study Schizophrenia....Pages 269-277
Combining Patient-Reprogrammed Neural Cells and Proteomics as a Model to Study Psychiatric Disorders....Pages 279-287
SILAC Mass Spectrometry Profiling: A Psychiatric Disorder Perspective....Pages 289-298
Preparation of Peripheral Blood Mononuclear Cells (PBMCs) as a Model for Proteomic Studies of Psychiatric Disorders....Pages 299-303
Proteomic Profiling of Skin Fibroblasts as a Model of Schizophrenia....Pages 305-311
Proteomic Profiling of the Pituitary Gland in Studies of Psychiatric Disorders....Pages 313-319
Development of an Assay for Measuring Proprotein-Conversion Activity on a Multiplex Magnetic Bead-Based Array Platform....Pages 321-326
Phenotyping Multiple Subsets of Immune Cells In Situ in Formalin-Fixed, Paraffin-Embedded Tissue Sections....Pages 327-338
Lab-on-a-Chip Proteomic Assays for Psychiatric Disorders....Pages 339-349
Development of a User-Friendly App for Testing Blood Coagulation Status in Schizophrenia Patients....Pages 351-360
Front Matter....Pages 361-361
Proteomic Approaches to Enable Point-of-Care Testing and Personalized Medicine for Psychiatric Disorders....Pages 363-370