Proteome and Protein Analysis

Front Matter....Pages I-XI
Front Matter....Pages 1-1
Strategies and Methods for Proteome Analysis....Pages 3-14
Protein Sequencing or Genome Sequencing. Where Does Mass Spectrometry Fit into the Picture?....Pages 15-27
Reversible Negative Staining of Protein on Electrophoresis Gels by Imidazole-Zinc Salts: Micropreparative Applications to Proteome Analysis by Mass Spectrometry....Pages 29-52
Direct Analysis of Protein Complexes....Pages 53-64
De novo Sequencing of Proteins With Mass Spectrometry Using the Differential Scanning Technique....Pages 65-79
Characterization of Gel Separated Proteins....Pages 81-87
Front Matter....Pages 89-90
Protein Structure and Dynamics by NMR in Solution....Pages 91-116
Elucidation of Functionally Significant Structural Modifications by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry with Post-Source Decay Analysis....Pages 117-138
Detection of Specific Zinc Finger Peptide Complexes with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry....Pages 139-147
Epitope Mapping for the Monoclonal Antibody that Inhibits Intramolecular Flavin to Heme Electron Transfer in Flavocytochrome b 2 from Baker’s Yeast (L-Lactate Dehydrogenase)....Pages 149-155
A MALDI-TOF Mass Spectrometry Approach to Investigate the Defense Reactions in Drosophila melanogaster , an Insect Model for the Study of Innate Immunity....Pages 157-174
A New Method for the Isolation of the C-Terminal Peptide of Proteins by the Combination of Selective Blocking of Proteolytic Peptides and Cation Exchange Chromatography....Pages 175-187
The Folding Pathway of Disulfide Containing Proteins....Pages 189-197
Cavity Supported HPLC of Cis/Trans Isomers of Proline Containing Peptides Using Cyclodextrins and Calixarenes....Pages 199-210
The Perception of Hydrophobic Clusters in the Native and Partially Unfolded States of a Protein....Pages 211-224
Structure, Dynamics and Function of the Proton Pump Bacteriorhodopsin....Pages 225-240
Structural Characterisation of Porcine Seminal Plasma Psp-I/Psp-II, a Paradigm Spermadhesin Molecule Built by Heterodimerization of Glycosylated Subunits....Pages 241-250
Expression and Characterization of Saposin-Like Proteins....Pages 251-254
Front Matter....Pages 255-255
A Structural Model for the P53 Complex with DNA Response Elements: Implications for P53 Function and Future Research Directions....Pages 257-272
Overproduction, Purification and Structural Studies on the Zn Containing S14 Ribosomal Protein from Thermus thermophilus ....Pages 273-288
Front Matter....Pages 255-255
Biomolecular Interaction of Matrix Metalloproteinases and Their Inhibitors TIMPs....Pages 289-300
Front Matter....Pages 301-301
Enzymatic and Chemical Deblocking of N-Terminally Modified Proteins....Pages 303-319
Exploring Functions for Glycosylation in Host Defence using Novel Oligosaccharide Sequencing Technology....Pages 321-339
Proteoglycans: Biological Roles and Strategies for Isolation and Determination of Their Glycan Constituents....Pages 341-363
Back Matter....Pages 365-372