Progress in Plant Protoplast Research: Proceedings of the 7th International Protoplast Symposium, Wageningen, the Netherlands, December 6–11, 1987

Front Matter....Pages I-XVII
Progress in Plant Protoplast Research....Pages 1-5
Obstacles and Perspectives in Protoplast Research....Pages 7-14
Aspects of Protoplast Culture and Plant Regeneration....Pages 15-25
Recent Developments in the Regeneration of Agronomically Important Crops from Protoplasts....Pages 27-31
Selection, Experimental Manipulation and Culture of Single Protoplasts....Pages 33-34
Uniparental, Fusant, and Chimeric Plants Regenerated from Protoplasts after Streak Plating in Agarose Gels....Pages 35-37
Electroporation-Mediated Enhancement of Plant Protoplast Division and Plant Regeneration....Pages 39-40
Selection of Potato Cell Suspension Protoplast Subpopulation by Rhodamine 123....Pages 41-42
Protoplast Culture Of Dioscorea Opposita Thunb. (D. Batatas DECNE)....Pages 43-44
Isolation and Culture of Buckwheat (Fagopyrum Esculentum Moench.) Callus Protoplasts....Pages 45-46
Partial Purification of a Cellulase Used for Protoplast Isolation from Barley ( Hordeum vulgare )....Pages 47-48
Plant Regeneration from Cell Suspension Protoplasts of Festuca Arundinacea Schreb., Lolium Perenne L. and L. Multiflorum Lam....Pages 49-52
Isolation, Culture and Regeneration of Lolium Perenne and Lolium Multiflorum Protoplasts....Pages 53-54
Mature Plant Regeneration from Cultivated Protoplasts of Rice ( Oryza Sativa L.)....Pages 55-56
The Plants Regenerated from Protoplasts of Suspension Cells of Rice(Oryza Sativa L)....Pages 57-58
Regeneration of Plantlets from Protoplasts of Poa Pratensis L. (Kentucky Bluegrass)....Pages 59-60
Somatic Embryogenesis and Plant Regeneration from Protoplast of Maize ( Zea mays L.)....Pages 61-62
Protoplasts from Actinidia Deliciosa (Kiwi): Obtention and Culture....Pages 63-64
Colony Formation from Cell Suspension Culture Protoplasts of Kiwifruit....Pages 65-66
Isolation and Culture of Grapevine Protoplasts (Vitis Vinifera L.)....Pages 67-68
Studies Towards a Protoplast Isolation and Culture Procedure in Sugarbeet ( Beta Vulgaris L.) Applicable to a Variety of Genotypes....Pages 69-70
Improvement of Protoplast Culture in Helianthus annuus (L)....Pages 71-72
Factors Influencing Protoplasts Plating Efficiency and Direct Somatic Embryogenesis Induction in Sunflower ( Helianthus Annuus L.)....Pages 73-74
Comparison of Sunflower Protoplast Development in Liquid or Solidified Media....Pages 75-76
Plant Regeneration from Protoplasts of Rudbeckia and Dimorphotheca Species ( Compositae )....Pages 77-78
Optimization of lettuce calli regeneration from protoplasts....Pages 79-80
Optimal Density and Osmolality of Protoplasts for Improved Cultural Performance....Pages 81-82
Development of Protoplast Culture Procedures in Brassica Crops Suitable for in Vitro Selection Herbicide Resistance Mutants and Cybrids....Pages 83-84
Somatic Embryogenesis in Stationary Phase Suspension Cultures Derived from Hypocotyl Protoplasts of Brassica Napus L.....Pages 85-90
Plant Regeneration from Cucumber Protoplasts....Pages 91-94
Callus Formation from Mesophyll Protoplasts of Forage Legume Astragalus Huangheensis Fu Et Lin....Pages 95-96
Preliminary Studies of Lupinus Protoplasts....Pages 97-98
Regeneration and Differentiation of Protoplasts in Grain Legumes....Pages 99-100
Plant Regeneration from Strawberry (Fragaria Ananassa) Mesophyll Protoplasts....Pages 101-102
Isolation and Culture of Apple Mesophyll Protoplasts....Pages 103-104
Rhizogenesis as an Intermediate Step towards Plant Regeneration of Protoplasts of Sour Cherry (Prunus Cerasus L.), Rosaceae....Pages 105-106
Isolation and Culture of Protoplasts from Totipotent Cell Cultures of Populus Ciliata ....Pages 107-108
A Protoplast System for Populus Which will Support Genetic Engineering Research of Tree Species....Pages 109-110
Factors Influencing Digitalis Obscura Mesophyll Protoplast Development....Pages 111-112
Cotyledon Protoplasts from Solanum Melongena L . Regeneration to Fertile Plants....Pages 113-114
Isolation, Culture and Regeneration of Leaf Protoplasts in the Genus Capsicum....Pages 115-116
Isolation and Properties of Protoplasts of Theobroma Cacao L.....Pages 117-118
Plantlet Regeneration from Protoplasts of Levisticum Officinale Koch.and Its Cytological Investigation....Pages 119-120
Differences in developmental morphology of protoplasts from two cell lines of white spruce ( Picea glauca )....Pages 121-122
Protoplast Development from Porphyra Linearis — An Edible Marine Red Alga....Pages 123-124
Structure and Physiology of the Protoplast Plasma Membrane....Pages 125-131
Why do Mesophyll Protoplasts Dedifferentiate?....Pages 133-134
Release of Phytotoxic Factors from Plant Cell Walls during Protoplast Isolation....Pages 135-136
Role of Peroxidase in Protoplast Development: The Activity and Molecular Forms of Peroxidase in Mitogenic and Non-Mitogenic Protoplasts....Pages 137-138
Measurement of Cytoplasmic Ca 2+ and H + in Barley Aleurone Protoplasts....Pages 139-142
The Effect of Secretion on Lipids and their Distribution in Barley Aleurone Protoplasts....Pages 143-144
Incidence of Growth Regulators on RNA/ DNA Levels in Protoplasts as Analysed by Acridine Orange Staining and Flow Cytometry....Pages 145-146
IAA, ABA Content and Metabolism in Maize Root Protoplasts....Pages 147-148
Some Metabolic Peculiarities of Isolated Protoplasts....Pages 149-150
Gene Transfer by Individual Manipulation of Plant Cells....Pages 151-157
Potentials of Protoplast Fusion in Plant Breeding Programmes....Pages 159-168
Use of Protoplasts on Plant Breeding: Cytoplasmic Aspects....Pages 169-176
Protoplast Fusion between Eruca Sativa and Brassica Napus — Analysis of Regenerated Plants....Pages 177-178
Fertility and Chromosome Stability in Brassica Napus Resynt-Hesised by Protoplast Fusion....Pages 179-180
Involvement of Protoplast Fusion in Cauliflower Breeding....Pages 181-182
Protoplast Fusion as a Method of Increasing Cytoplasmic Heterogeneity in Brassica Napus ....Pages 183-184
Selection of a Universal Hybridizer in Sinapis Turgida Del. and Regeneration of Plantlets from Somatic Hybrids with Brassica Species....Pages 185-186
Towards the Introduction of Cytoplasmic Male Sterility (CMS) into Brassica Napus Through Protoplast Fusion....Pages 187-188
Production of Somatic Hybrid Plants, ‘Brassicomoricandia’, Through Protoplast Fusion between Moricandia Arvensis and Brassica Oleracea ....Pages 189-190
The Use of Haploid Protoplast Fusion to Combine Cytoplasmic Atrazine Resistance and Cytoplasmic Male Sterility in Brassica Napus ....Pages 191-194
Somatic Hybridization of Diploid Potato Lines, a Tool in Potato Breeding....Pages 195-196
Somatic Hybridization between Anther-Derived Dihaploid Lines of Solanum Tuberosum L. (Potato)....Pages 197-198
Fusion of Dihaploid Solanum Tuberosum Protoplasts and Trials of Hybrid Selection with Phytotoxins....Pages 199-200
Production and Field Assessment of Somatic Hybrids between Dihaploid Solanum Tuberosum and Solanum Brevidens ....Pages 201-201
Fusion of Solanum tuberosum L. cv. Explorer(Potato) and Solanum melongena L. cv. Imperial Black Beauty(Eggplant) Protoplasts and Subsequent Callus Regeneration....Pages 203-204
“Somatic Fusions of Solanum Species”....Pages 205-207
Flow Cytometric Sorting of Potato Heterokaryons with Inhibition of Cell Wall Synthesis before Sorting....Pages 209-210
An Improved Peg Fusion Procedure for Flow Sorting of Plant Protoplasts....Pages 211-212
Regeneration and Evaluation of Somatic Hybrid Plants between Solanum Tuberosum and Lycopersicon Pimpinellifolium ....Pages 213-214
Studies on Gene Mapping in the Potato Using Somatic Hybrids of Solanum Tuberosum L. + Nicotiana Plumbaginifolia ....Pages 215-218
Interspecific Expression of AEC-Resistance in Somatic Hybrids of AEC-Resistant Solanum Tuberosum and Nitrate-Reductase-Deficient Nicotiana Plumbaginifolia....Pages 219-220
Interspecific Protoplast Fusion for the Transfer of Atrazine Resistance from Solanum Nigrum to Tomato ( Lycopersicon Esculentum .L)....Pages 221-224
Somatic hybridization between tomato and other Lycopersicon or Solanum species....Pages 225-226
Transfer of Regeneration Capacity from Lycopersicon Peruvianum to L. Esculentum by Protoplast Fusion....Pages 227-230
Somatic Hybridization in Lycopersicon; Using Gamma-Irradiated Nicotiana Tabacum SR-1 or Lycopersicon Peruvianum....Pages 231-232
Regeneration of Somatic Hybrid Plants between Tobacco ( Nicotiana Tabacum L.) and Black Nightshade ( Solanum Nigrum L.)....Pages 233-234
The Use of Streptomycin Resistance and Chlorophyll Deficiency for Selection of Somatic Hybrids between Nicotiana Tabacum and N. Rustica ....Pages 235-236
Intra- and Interspecific Gametosomatic Hybridisation within the Genus Petunia....Pages 237-240
The Transfer of Cytoplasmic Male Sterility (CMS) by Intraspecific Somatic Hybridisation in Petunia Hybrida (Hort) VILM....Pages 241-242
Application of Protoplast Fusion to an Ornamental Breeding Programme....Pages 243-244
Formation of Carrot Cybrids Based on Metabolic Complementation by Fusion between X-Irradiated Cells and Iodoacetamide-Treated Cells....Pages 245-248
Optimization of Conditions for Somatic Cybridization of Daucus Carota L....Pages 249-250
Isolation and Fusion of Apium Graveolens (Celery) and Levisticum Officinale (Lovage) Mesophyll Protoplasts....Pages 251-252
Electrofusion of Plant and Fungal Protoplasts....Pages 253-254
Regeneration and Analysis of Callus from Flow-Sorted Heterokaryons of Soybean ( Glycine Max ) and G. Canescens ....Pages 255-256
Interspecific Somatic Hybridization Studies in the Genus Lotus ....Pages 257-258
Interspectfic Hybrid Lines of M. Sativa and M. Arborea by Protoplast Electrofusion....Pages 259-260
Electrofusion and Electroporation of Sugar Beet ( Beta Vulgaris L.) Protoplasts....Pages 261-262
The Production and Electrofusion of Beta Cytoplasts....Pages 263-264
Inactivation of Sugarbeet Protoplasts Using Acridin Orange, an Agent for Late Selection of Fusion Products....Pages 265-266
Mitochondrial DNA Amplification in Somatic Hybrid Cells of Grasses....Pages 267-268
Plant Regeneration from Electrofused Protoplasts of Chrysanthemum Morifolium and Salpiglossis Sinuata ....Pages 269-270
Electrofusion of Chemically-Aggregated Protoplasts Derived from Mesophyll Tissue and Habituated Cells of Catharanthus Roseus....Pages 271-272
Studies on the Transfer of the Mitochondrial Genome Through Protoplast Fusion in Rice....Pages 273-274
Increase of Genetic Variability by Asymmetric Cell Hybridization and Isolated Chromosome Transfer....Pages 275-281
Cell-Mediated Gene Transfer....Pages 283-285
Protoplast Fusion of Kanamycin Resistant, CNX - Nicotiana Plumba-Ginifolia with Streptomycin Resistant N. Tabacum (SR1) and the Effect of Irradiation of the Tabacum Parent....Pages 287-288
Selecton for Resistance to Phoma Lingam Using Pycnospore Infections of Plants in-Vivo and Sirodesmin PL as Selective Agent in-Vitro ....Pages 289-292
Induction of Micronuclei by Amiprophos-Methyl and their Application for Transfer of Single, Specific Chromosomes in Plant Cells....Pages 293-296
Isolation of Microprotoplasts....Pages 297-298
Direct Cell to Cell Transfer of Mitochondria by Microinjection....Pages 299-300
Classification and Sorting of Plant Chromosomes Using Flow Cytometry....Pages 301-302
Application of Flow Cytometry for the Isolation of Metaphase Chromosomes from Protoplasts of Nicotiana Plumbaginifolia ....Pages 303-304
Electroporation for DNA Delivery to Plant Protoplasts....Pages 305-310
Electromanipulation of Protoplasts....Pages 311-314
Plant Protoplast Electropermeabilization: Role of Electrical and Biological Parameters....Pages 315-316
The Fusogenic State of Plant Protoplasts as Related to DYE/DNA Uptake....Pages 317-318
Genetic Manipulation of Plant Cells and Organelles with a Laser Microbeam....Pages 319-322
Electric Field Mediated DNA Uptake and Gene Expression in Mesophyll, Suspension Culture, and Tuber Protoplasts of Wild and Cultivated Potato....Pages 323-324
Introduction of Selectable Markers into Different Diploid Potato ( Solanum Tuberosum L .) Genotypes by Direct Gene Transfer....Pages 325-327
Direct Gene Transfer in Petunia Hybrida Protoplasts: Evidence for Co-Transformation....Pages 329-330
Electroporation of Tobacco Protoplasts: A Model System to Study Plant Gene Expression....Pages 331-332
Infection of Tobacco Protoplasts with RNA of a Pepper Strain of Tobacco Mosaic Virus by Electroporation....Pages 333-334
Comparison of Direct Gene Transfer into Nicotiana Protoplasts by Electroporation and Mg 2+ /PEG Methods....Pages 335-336
Quantification of Transient Expression Levels of Genes Transferred to Plant Protoplasts by Electroporation....Pages 337-338
Gene Transfer by Electroporation in Alnus Incana Protoplast....Pages 339-340
Transformation of Rice Protoplasts by Direct DNA Uptake....Pages 341-342
Isolation, Culture and Morphogenesis from Wheat Protoplasts, and Study of Expression of DNA Constructs by Direct Gene Transfer....Pages 343-346
UV Inducible Transient Expression of Chimeric CHS-NPTII in Parsley Proto — Plasts....Pages 347-348
Modification of Plants Using Intracellular Associations of L-Form Bacteria....Pages 349-350
Transformation of Brassica Nigra Through Protoplast Cocultivation with Agrobacterium Tumefaciens....Pages 351-352
Transformation of Brassica Species Via Direct Gene Transfer....Pages 353-354
Genetic Transformation of Diploid Solanum Tuberosum by Direct DNA Transfer and Mediated by Agrobacterium Tumefaciens....Pages 355-356
Optimization of Direct Gene Transfer in Tomato....Pages 357-358
Transformation Studies in Tobacco, Moth Bean and Brassica Species....Pages 359-362
Transposition and Regulation of the En Elements of Maize Assayed in Transformed Tobacco....Pages 363-364
Initiation of Cell Suspensions, Protoplast Culture, and Direct Gene Transfer to Protoplasts of Secale cereale L. (rye)....Pages 365-367
Protoplast Culture and DNA Uptake in Echinocloa....Pages 369-370
Transient Expression of Foreign Genes in Wheat Protoplasts....Pages 371-372
Protoplast culture and transformation studies of Triticale ( x Triticosecale Wittmack)....Pages 373-376
Analysis of the Zea Mays Sucrose Synthase Promotor by Transient Gene Expression in Maize Protoplasts....Pages 377-378
Protoplast Culture of 2n S. Tuberosum as a Source of Mutant Phenotypes....Pages 379-380
Cytoplasmic or Nuclear Control of Male Sterility in Protoplast-Derived Plants of Nicotiana Sylvestris ....Pages 381-382
Selection and Characterization of Nitrate Reductase-Deficient Mutants of Nicotiana Plumbaginifolia ....Pages 383-385
Isolation of Temperature-Sensitive Auxin-Requiring Variants of Nicotiana Plumbaginifolia....Pages 387-388
The use of phosphinothricin resistance as a selectable marker in tobacco protoplast transformation....Pages 389-390
Selection for Salt/Drought Tolerance Using Isolated Protoplasts and Protoplast-Derived Calli of Colt Cherry (Prunus Avium X Pseudocerasus)....Pages 391-392
Field Tests of Progeny from Protoplast Regenerated Oilseed Rape....Pages 393-394
Studies of lettuce plants regenerated from protoplasts....Pages 395-396
Somaclonal Variations for Indole Alkaloid Accumulation in Protoclones Derived from an Habituated Strain of Catharanthus Roseus....Pages 397-398
Somaclonal Variations of Dihydrofuroquinoline Alkaloids in Cell Lines Issued from Strains and Leaf Mesophyll of Choisya Ternata by Protoplast Cloning....Pages 399-400
Some Aspects of Salinity Tolerance of Calli Derived from Leaf Tomato Protoplasts....Pages 401-402
Proto-Variation in Protoplast Derived Brassica Napus Plants....Pages 403-404
Back Matter....Pages 405-412