Progress in Plant Cellular and Molecular Biology: Proceedings of the VIIth International Congress on Plant Tissue and Cell Culture, Amsterdam, The Netherlands, 24–29 June 1990

Front Matter....Pages i-xiii
Front Matter....Pages 1-1
Micropropagation in Crop Species....Pages 3-12
Genetic Preservation in Vitro ....Pages 13-22
Current Research in Biotechnology with Application to Plant Breeding....Pages 23-32
Plant Regeneration from Embryogenic Calli, Cell Suspension Cultures and Protoplasts of Triticum aestivum L. (Wheat)....Pages 33-37
Tissue, Cell, and Protoplast Culture of Maize ( Zea mays L.)....Pages 38-43
Antioxidants Influence the Plating Efficiency and Microcallus-Growth of Protoplasts in Lolium Perenne L. ....Pages 44-49
Enhancement of Sugar Yield: A Molecular Biological Approach....Pages 50-55
Applications of the Particle Gun in Plant Biology....Pages 56-66
X-Irradiation and Co-Transformation of Petunia “Mitchell” Protoplasts with Two Antibiotic Resistance Genes....Pages 67-72
Gene Transfer to Barley....Pages 73-78
Promoterless Reporter Genes and Their Use in Plant Gene Transformation....Pages 79-84
Transformation of Cucumber Via Agrobacterium Tumefaciens ....Pages 85-90
Rejuvenation and Micropropagation....Pages 91-101
Genotype-Phenotype Interplay in Micropropagation....Pages 102-107
Effect of Mineral Nutrition on the Growth and Multiplication of in vitro Cultured Plants....Pages 108-113
Rapid regeneration of sugarbeet ( Beta vulgaris L.) plants from in vitro cultures....Pages 114-118
Morphogenesis of Gladiolus Buds in Bioreactors — Implication for Scaled-up Propagation of Geophytes....Pages 119-124
Dormancy Development in Lily Bulblets Propagated in Vitro ....Pages 125-130
Do We Understand Somaclonal Variation?....Pages 131-141
Gene Expression in Salt Tolerant Alfalfa Cell Cultures and the Salt Tolerant Plants Regenerated from These Cultures....Pages 142-147
Front Matter....Pages 1-1
Glyphosate Resistance and Gene Amplification in Selected Daucus carota Suspension Cultures....Pages 148-152
RFLP-analysis of Rice Plants Regenerated from Tissue Cultures....Pages 153-156
 In Vivo and in Vitro Mutagenesis of a Brassica Napus Acetolactate Synthase Gene for the Production of Herbicide Resistance....Pages 157-162
Cell Culture of Alien Chromosome Addition Lines to Induce Somatic Recombination and Gene Introgression....Pages 163-168
Antibiotic Resistant Mutants of Solanum Nigrum ....Pages 169-174
Establishing a Tomato Transposon Tagging System with Heterologous Transposons....Pages 175-182
The Use of the Polymerase Chain Reaction to Detect Specific Sequences in Transformed Plant Tissues....Pages 183-188
Differential Expression of Agropine Synthase Gene during Morphogenesis in Vitro....Pages 189-194
Intrinsic GUS-Like Activity....Pages 195-200
Field Testing of Cucumber Plants Which Express the CMV Coat Protein Gene: Field Plot Design to Test Natural Infection Pressures....Pages 201-206
Two Years of Transgenic Flax Field Tests: What do They Tell Us?....Pages 207-212
From Microspore to Embryoid: Faces of the Angiosperm Pollen Grain....Pages 213-221
Two Pathways in Pollen Protoplast Culture: Cell Divisions and Tube Growth....Pages 222-227
Biochemical and Cytological Changes in Young Tobacco Pollen during in vitro Starvation in Relation to Pollen Embryogenesis....Pages 228-233
Molecular and Physiological Differentiation of Pollen in-Vitro....Pages 234-238
Sex Determination in the Dioecious Melandrium : The in Vitro Culture Approach....Pages 239-243
Transformation of Pollen....Pages 244-251
In Vitro Fertilization of Single, Isolated Gametes, Transmission of Cytoplasmic Organelles and Cell Reconstitution Of Maize ( Zea Mays L.)....Pages 252-257
Anther Culture Selection to Enhance Snow Mold Disease Resistance in Winter Wheat....Pages 258-263
Haploid Suspension and Protoplast Culture from Isolated Microspores of Maize....Pages 264-269
Front Matter....Pages 1-1
Embryo Rescue of Interspecific Hybrids of Brassica Spinescens X Brassica Campestris and DNA Analysis....Pages 270-274
Haploids from Unpollinated Ovaries of Allium Cepa — Single Plant Screening, Haploid Determination, and Long Term Storage....Pages 275-279
Partial Genome Transfer in Interspecific Tomato Hybrids....Pages 280-285
Transfer of Resistance to PLRV, PVX and PVY from S.brevidens to Potato by Somatic Hybridisation: Characterisation and Field Evaluation....Pages 286-292
Transfer of Tobacco Mosaic Virus Resistance by Asymmetric Protoplast Fusion....Pages 293-298
Improvements of the Brassica Crops by Transfer of Genes from Alien Species Via Somatic Hybridization....Pages 299-304
Front Matter....Pages 305-305
Mechanisms of Somatic Embryogenesis in Celll Cultures— Physiology, Biochemistry and Molecular Biology....Pages 307-313
Lipid Synthesis and Transfer....Pages 314-323
Biosynthesis of Triacylglycerols in Plant Cell and Embryo Cultures Their Significance in the Breeding of Oil Plants....Pages 324-331
Lipid Composition of Plasma Membrane from Crown Gall Tumour Cells in Vitro....Pages 332-337
Intracellular Pi-Compartmentation during Phosphate Starvation-Triggered Induction of an Extracellular Ribonuclease in Tomato Cell Culture....Pages 338-342
Aerobic Hexose Metabolism in Embryogenic Cell Lines of Daucus Carota Studied by 13 C NMR Using 13 C-Enriched Substrates....Pages 343-348
Are Two Localization Sites of Nitrate Reductase Responsible for the Differential Expression in Tobacco Cells during the Growth Cycle ?....Pages 349-354
Phytochrome in Plant Cell Cultures....Pages 355-365
Blue-Light Effects on Gene Expression in Plant-Cell Cultures....Pages 366-372
Light-Dependent Growth and Differentiation Processes in Photoautotrophic Cell Cultures....Pages 373-378
Light Stimulated Cell Proliferation and Glyoxalase Activity in Callus Cultures of Amaranthus Paniculatus .....Pages 379-384
Spatial and Temporal Expression of Regulatory Genes in Zea mays ....Pages 385-390
Developmental Mutants in Carrot....Pages 391-397
Tissue and Cell-Specific Expression of Genes Encoding Carrot Extracellular Proteins....Pages 398-401
Front Matter....Pages 305-305
Microspore-derived embryos: A tool for studies in regulation of gene expression in zygotic embryos....Pages 402-406
Molecular changes associated with the commitment phase of microspore embryogenesis....Pages 407-409
Expression of Chimeric Genes in Developing Embryos Derived from Isolated Microspores from Transgenic Brassica Napus Plants....Pages 410-415
Detection of Androgenesis-Related Proteins in Maize....Pages 416-421
Significant Higher Proportions of 1BL-1RS Wheat-Rye Translocation Lines Among Doubled Haploid Progenies Derived from F1 Hybrids between Translocated and Non-Translocated Wheat Cultivars....Pages 422-426
Differences in Responses to 2,4-Dichlorophenoxy Acetic Acid (2,4-D) Treatment between Embryogenic and Non-Embryogenic Lines of Alfalfa....Pages 427-436
The Commitment to Embryogenesis, A Cytological Approach....Pages 437-442
Immunocytochemical Localization of Calmodulin During Carrot Somatic Embryogenesis....Pages 443-448
pH Control of Carrot Somatic Embryogenesis....Pages 449-453
Somatic Embryogenesis in Celery: Induction, Control and Changes in Polyamines and Proteins....Pages 454-459
Production and Characterization of Monoclonal Antibodies against Marker Proteins for Somatic Embryogenesis in Pea (Pisum sativum L.)....Pages 460-465
Signal Transduction at the Membrane Level of Plant Cells....Pages 466-479
Inhibition of Polyamine Biosynthesis Affects the Rhizogenic Response in Tobacco Thin Cell Layers....Pages 480-485
Plant Growth Regulator Manipulations in Sugar Beet....Pages 486-491
Regulation of Cytokinin Catabolism in Tobacco Callus Cultures....Pages 492-497
Differential Sensitivity to Auxin at the Plasma Membrane Level....Pages 498-503
Rôles of the Primary Cell Wall in Morphogenesis....Pages 504-513
Signals Influencing Cell Elongation, Cell Enlargment, Cell Division And Morphogenesis....Pages 514-519
Effect of a Xyloglucan-Derived Nonasaccharide on Carrot Protoplasts....Pages 520-525
Characterization of GP57, an Auxin-Regulated and Dermaltissue-Localized Extracellular Glycoprotein of Carrot....Pages 526-531
Front Matter....Pages 305-305
Molecular Aspects of the Cell Cycle in Catharanthus Roseus Synchronous Cell Division Cultures....Pages 532-537
Microtubules and the First Cell Cycle in Cultured Mesophyll Protoplasts of Nicotiana ....Pages 538-543
Front Matter....Pages 545-545
Possibilities for Improving Yields of Secondary Metabolites in Plant Cell Cultures....Pages 547-556
The Toxic Compartment of Plant Cells....Pages 557-566
Transport and Accumulation of Secondary Metabolites....Pages 567-576
Accumulation of Ajmalicine and Serpentine in Vacuoles Isolated from Catharanthus Roseus Cell Suspension Cultures....Pages 577-581
Recovery of Secondary Metabolites with Minimal Loss of Cell Viability....Pages 582-586
Cellular Aspects of Thiophene Accumulation in Relation to Root Differentiation....Pages 587-591
Possibilities of Finding New Products from Plant Cell Cultures....Pages 592-600
Large-Scale Production of New Biologically Active Compounds in Plant-Cell Cultures....Pages 601-608
Terpene Production by Liverworts of in Vitro Cultures....Pages 609-613
Immobilized Ginkgo Biloba Cell Cultures....Pages 614-618
Production of Terpenes by Transformed Differentiated Shoot Cultures of Mentha Piperita Citrata and M.Piperita Vulgaris ....Pages 619-624
Isolation and Distribution of 7β-Hydroxyhyoscyamine in Solanaceous Plants....Pages 625-630
Bioconversion of Xenobiotics (Pesticides) in Plant Cell Cultures....Pages 631-639
Bioconversion of Precursors Occurring in Plants and of Related Synthetic Compounds....Pages 640-649
Bioconversion of Xenobiotics in Different Plant Systems — Cell Suspension Cultures, Root Cultures and Intact Plants....Pages 650-655
Metabolism of Polychlorinated Biphenyls (PCBs) by Plant Tissue Cultures....Pages 656-660
Metabolism of Metribuzin in Tomato Cell Cultures by a Glucosyltransferase....Pages 661-666
Conversion of Glucosinolate Synthetic Precursors in Brassica Cultures....Pages 667-672
Front Matter....Pages 545-545
Possibilities, Problems, and Pitfalls of Large-Scale Plant Cell Cultures....Pages 673-681
Biosynthesis of Sanguinarine by Elicitation of Surface Immobilized Cells of Papaver Somniferum L.....Pages 682-688
The Effect of Continuous High Shear Stress on Plant Cell Suspension Cultures....Pages 689-693
Application of the Liquid-Impelled Loop Reactor for the Production of Anthraquinones by Plant Cell Cultures....Pages 694-699
The Cultivation of Transformed Roots from Laboratory to Pilot Plant....Pages 700-705
Production of Deacetyllanatoside C by Digitalis Lanata Cell Cultures....Pages 706-711
Use of Genetic Engineering to Improve Yields in Cell Cultures, e.g. (Anti)Sense DNA Technology....Pages 712-716
Strategies to Improve Yields of Secondary Metabolites to Industrially Interesting Levels....Pages 717-725
Selection of High-Yielding Cell Lines of Tagetes Using Flow Cytometry....Pages 726-731
The Effects of Over-Expressing the Yeast Ornithine Decarboxylase Gene upon the Nicotine and Polyamine Levels in Transformed Roots of Nicotiana Rustica ....Pages 732-737
Semisynthetic Production of Vinblastine Involving Cell Cultures of Catharanthus Roseus and Chemical Reaction....Pages 738-743
Principles Regulating Formation and Activity of Secondary Metabolic Enzymes in Plant Tissue and Cell Cultures....Pages 744-753
Enzymology of Alkaloid Production in Plant Cell Cultures....Pages 754-762
Affecting Secondary Product Formation in Suspension and Hairy Root Cultures — a Comparison....Pages 763-768
Purification of Cytochrome P-450-Dependent Geraniol-10-Hydroxylase from a Cell Suspension Culture of Catharanthus Roseus ....Pages 769-774
Molecular Cloning, and Tissue- and Cell-Specific Expression of Hyoscyamine 6β-Hydroxylase....Pages 775-780
Front Matter....Pages 781-781
Perspectives in Plant Cell and Tissue Culture in Developing Countries....Pages 783-788
Commercialisation of Agricultural Biotechnology in India: Opportunities, Progress and Problems....Pages 789-794
Impact of Tissue Culture Techniques on the Development of Agribusiness in Mexico....Pages 795-800
A Model for Comparing and Evaluating Biotechnological Innovations for Small-Scale Farmers in Developing Countries....Pages 801-810
Back Matter....Pages 811-811