Progranulin

Front Matter ....Pages i-xiii
Front Matter ....Pages 1-1
 A Brief Overview of Progranulin in Health and Disease  (Andrew Bateman, Siu Tim Cheung, Hugh P. J. Bennett)....Pages 3-15
Front Matter ....Pages 17-17
 Chromatographic Methods for the Purification of Granulin Peptides  (Andrew Bateman, Babykumari P. Chitramuthu, Hugh P. J. Bennett)....Pages 19-34
 Methods for Expression and Purification of Biologically Active Recombinant Progranulin  (Chun Yu Zhao, Andrew Bateman)....Pages 35-49
 Large-Scale Generation of Recombinant Granulin Peptides in E. coli  (Dmitri Tolkatchev)....Pages 51-64
 Nuclear Magnetic Resonance Spectroscopy in Analysis of Granulin Three-Dimensional Structure and Cysteine Bridging  (Dmitri Tolkatchev)....Pages 65-80
 Data Mining: Applying the AD&FTD Mutation Database to Progranulin  (Marc Cruts, Christine Van Broeckhoven)....Pages 81-92
Front Matter ....Pages 93-93
 Measurement of Circulating Progranulin (PGRN/GP88/GEP) by Enzyme-Linked Immunosorbent Assay and Application in Human Diseases  (Ginette Serrero, David Hicks)....Pages 95-105
 Immunohistochemical Detection of Progranulin (PGRN/GP88/GEP) in Tumor Tissues as a Cancer Prognostic Biomarker  (Ginette Serrero, David Hicks)....Pages 107-120
 Analysis of Progranulin-Mediated Akt and MAPK Activation  (Shi-Qiong Xu, Simone Buraschi, Ryuta Tanimoto, Manuela Stefanello, Antonino Belfiore, Renato V. Iozzo et al.)....Pages 121-130
 Mouse Monoclonal Antibodies Against Progranulin (PGRN/GEP) as Therapeutics in Preclinical Cancer Models  (Chi Wai Yip, Phyllis F. Y. Cheung, Nicholas C. L. Wong, Sze Wai Fung, Siu Tim Cheung)....Pages 131-144
 Methods to Analyze the Role of Progranulin (PGRN/GEP) on Cancer Stem Cell Features  (Phyllis F. Y. Cheung, Siu Tim Cheung)....Pages 145-153
 Methods to Study the Role of Progranulin in the Tumor Microenvironment  (Moshe Elkabets, Samuel Brook)....Pages 155-176
Front Matter ....Pages 177-177
 Methods to Investigate the Molecular Basis of Progranulin Action on Neurons In Vivo Using Caenorhabditis elegans  (James Julian Doyle, J. Alex Parker)....Pages 179-191
 The Use of Caenorhabditis elegans to Study Progranulin in the Regulation of Programmed Cell Death and Stress Response  (Tsung-Yuan Hsu, Victoria J. Butler, Aimee W. Kao)....Pages 193-206
 Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function  (Babykumari P. Chitramuthu, Hugh P. J. Bennett)....Pages 207-231
 Methods to Investigate the Molecular Basis of Progranulin Actions on Brain and Behavior In Vivo Using Knockout Mice  (Jan Boddaert, Hans Wils, Samir Kumar-Singh)....Pages 233-253
 Methods to Investigate the Protection Against Neurodegenerative Disorders Provided by Progranulin Gene Transfer in the Brain  (Jackalina M. Van Kampen)....Pages 255-267
 The Interaction Between Progranulin with Sortilin and the Lysosome  (Xiaolai Zhou, Peter M. Sullivan, Daniel H. Paushter, Fenghua Hu)....Pages 269-288
 Methods to Study the Role of Progranulin in Preimplantation Mouse Embryo Development  (Laura Diaz-Cueto, Fabian Arechavaleta-Velasco, George L. Gerton)....Pages 289-304
 Establishment of a Modified Collagen-Induced Arthritis Mouse Model to Investigate the Anti-inflammatory Activity of Progranulin in Inflammatory Arthritis  (Jian-Lu Wei, Chuan-Ju Liu)....Pages 305-313
 Methods for Studying the Function of Progranulin in Atherosclerosis Using Both Knockout Mice Models and In Vitro Studies  (Tohru Ohama, Ryota Kawase, Yasushi Sakata, Shizuya Yamashita)....Pages 315-328
 Methods to Investigate the Roles of Progranulin in Angiogenesis Using In Vitro Strategies and Transgenic Mouse Models  (Huishi Toh, Eugene Daniels, Andrew Bateman)....Pages 329-360
Back Matter ....Pages 361-363