Pre-Clinical Models: Techniques and Protocols

Front Matter ....Pages i-xiii
Front Matter ....Pages 1-1
 A Guide to Mass Spectrometry-Based Quantitative Proteomics  (Bradley J. Smith, Daniel Martins-de-Souza, Mariana Fioramonte)....Pages 3-39
 Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic, and Environmental Factors  (Keiko Iwata)....Pages 41-48
 Monosodium Glutamate (MSG)-Induced Animal Model of Type 2 Diabetes  (Zahra Bahadoran, Parvin Mirmiran, Asghar Ghasemi)....Pages 49-65
Front Matter ....Pages 67-67
 The Elevated Plus Maze Test for Measuring Anxiety-Like Behavior in Rodents  (Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai)....Pages 69-74
 The Forced Swim Test for Depression-Like Behavior in Rodents  (Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai)....Pages 75-80
 Object Burying Test for Assessment of Obsessive Compulsive Behaviors in Mice  (Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai)....Pages 81-85
 The Nest Building Test in Mice for Assessment of General Well-Being  (Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai)....Pages 87-91
 Free Dyadic Social Interaction Test in Mice  (Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai)....Pages 93-97
 The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behavior  (Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai)....Pages 99-103
 The Y-Maze for Assessment of Spatial Working and Reference Memory in Mice  (Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai)....Pages 105-111
 Maturation of a Human Oligodendrocyte Cell Line  (Gabriela Seabra, Valéria de Almeida, Daniel Martins-de-Souza)....Pages 113-121
 Monitoring Age-Related Changes in the Lactate/Pyruvate Ratio Using a Colorimetric Assay in a C. elegans Model of Increased Life Span  (Sumino Yanase, Kayo Yasuda, Naoki Ishii)....Pages 123-132
 Isolation and Characterization of Different Mesenchymal Stem Cell Populations from Rat Femur  (Frans Alexander Jacobs, Mari van de Vyver, William Frank Ferris)....Pages 133-147
 Characterization of an Animal Model of Autism and Social Interaction  (Keiko Iwata)....Pages 149-155
 Assaying Reproductive Capacity in Female Rodents  (Catherine E. Aiken, Jane L. Tarry-Adkins, Susan E. Ozanne)....Pages 157-166
 Experimental Model of HindLimb Suspension-Induced Skeletal Muscle Atrophy in Rodents  (Gabriel Nasri Marzuca-Nassr, Kaio Fernando Vitzel, Gilson Masahiro Murata, José Luis Márquez, Rui Curi)....Pages 167-176
 An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes Using Flow Cytometry  (Yuji Takeda, Hironobu Asao, Ichiro Wakabayashi)....Pages 177-193
 Characterization of the db/db Mouse Model of Type 2 Diabetes  (Paul C. Guest, Hassan Rahmoune)....Pages 195-201
 Characterization of the Goto-Kakizaki (GK) Rat Model of Type 2 Diabetes  (Paul C. Guest)....Pages 203-211
 Characterization of Transplantable Insulinoma Cells  (Paul C. Guest)....Pages 213-222
 Preparation of Islets from Rat Pancreas and Assessment of Islet Function  (Paul C. Guest)....Pages 223-232
 Cryopreservation of Red Blood Cells  (Noha A. S. Al-Otaibi, Nigel K. H. Slater, Hassan Rahmoune)....Pages 233-238
 Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryopreservation  (Noha A. S. Al-Otaibi, Juliana S. Cassoli, Nigel K. H. Slater, Hassan Rahmoune)....Pages 239-247
 Reprogramming of Human Melanocytes and Melanoma Cells with Yamanaka Factors  (Hadiseh Taheri, Umut Cagin, Açelya Yilmazer)....Pages 249-261
 The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis  (Eleanor Cave, Nigel J. Crowther)....Pages 263-272
 Generation of Human Pyruvate Carboxylase Knockout Cell Lines Using Retrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study Its Metabolic Role in Cancer Research  (Khanti Rattanapornsompong, Jarunya Ngamkham, Tanit Chavalit, Sarawut Jitrapakdee)....Pages 273-288
 The Use of Primary Hepatocytes in Assessment of Drug Safety and Toxicity  (Paul C. Guest)....Pages 289-295
 OVA-Induced Allergic Airway Inflammation Mouse Model  (Mateus Casaro, Vanessa R. Souza, Fernando A. Oliveira, Caroline M. Ferreira)....Pages 297-301
 Description of Ovariectomy Protocol in Mice  (Vanessa R. Souza, Eduardo Mendes, Mateus Casaro, Ana Tada F. B. Antiorio, Fernando A. Oliveira, Caroline M. Ferreira)....Pages 303-309
 A Rat Eye Lens Model of Cataract Formation  (Paul C. Guest)....Pages 311-318
 A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue  (Guilherme Reis-de-Oliveira, Mariana Fioramonte, Daniel Martins-de-Souza)....Pages 319-328
 Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 β-Cell Line: A Preclinical Model for Type 2 Diabetes  (Jordan Rowlands, Nikita Walz, Joanne E. Rowles, Kevin N. Keane, Rodrigo Carlessi, Philip Newsholme)....Pages 329-340
Back Matter ....Pages 341-343