Plant Vacuoles: Their Importance in Solute Compartmentation in Cells and Their Applications in Plant Biotechnology

Front Matter....Pages i-xvi
Overview of Recent Developments....Pages 1-2
Vacuoles Today and Twenty Years Ago....Pages 3-6
Methodological and Other Aspects of Intact Mature Higher Plant Cell Vacuoles....Pages 7-19
Properties of Vacuoles as a Function of the Isolation Procedure....Pages 21-29
Highly Purified Tonoplast Fractions by Preparative Free-Flow Electrophoresis....Pages 31-42
Analysis of Vacuolar Polypeptides from Barley Mesophyll by Immunoblotting....Pages 43-50
A Hyperpolarization-Activated K + Current in Isolated Vacuoles of Acer Pseudoplatanus ....Pages 51-56
A Microcomputer-Based System for the Recording and Analysis of Fluorescence Transients from Membrane Vesicles....Pages 57-62
Benefits and Costs of Vacuolation....Pages 63-65
The Photosynthetic Potential and Growth of Photoautothrophic Cell Suspension Cultures from Chenopodium Rubrum : A Case Study....Pages 67-78
Vacuolar Accumulation of Organic Acids and their Anions in CAM Plants....Pages 79-87
Osmotic Factors Affecting the Mobilisation of Sucrose from Vacuoles of Red Beet Storage Root Tissue....Pages 89-94
Distribution of Potassium between Vacuole and Cytoplasm in Response to Potassium Deficiency....Pages 95-98
Vacuolar pH Level is Related to Photosynthesis in Isolated Mesophyll Protoplasts of Melilotus Alba ....Pages 99-99
Understanding Tonoplast Function: Some Emerging Problems....Pages 101-110
Analysis of the Electrical Fluctuations in Red Beet ( Beta Vulgaris L.) Vacuoles....Pages 111-120
Purification and Properties of α-Mannosidase from Vacuolar Membranes of Yeast Saccharomyces Cerevisiae ....Pages 121-125
Simultaneous Kinetic Measurements of Adenine Nucleotides in Isolated Vacuoles by HPLC Technique....Pages 127-133
Comparison of Two Methods for the Isolation of Vacuoles in Relation to Vacuolar ATPase Activity....Pages 135-141
NBD-PC: A Tool to Study Phospholipase Activity in Protoplasts....Pages 143-146
On the Existence of a Tonoplast-Bound ATPase....Pages 147-155
A Third-Category and a Fourth-Category H + -Phosphohydrolase at the Tonoplast....Pages 157-171
Structure and Function of the Subunits of the Vacuolar Membrane H + -ATPase of Saccharomyces Cerevisiae....Pages 173-178
Differential Susceptibilities of Tonoplast ATPase and PPase to Irreversible Inhibition by Chaotropic Anions....Pages 179-186
Generation of a pH-Gradient Across the Tonoplast of Vacuoles Isolated from Green Suspension Cells of Chenopodium Rubrum ....Pages 187-189
Mg, ATP-Dependent H + Transport in Vacuoles and Tonoplast Vesicles from Acer Pseudoplatanus Cells....Pages 191-198
The Effects of Anions on PP i -Dependent Proton Transport in Tonoplast Vesicles from Oat Roots....Pages 199-204
Changes of Vacuolar and Cytoplasmic pH Associated with the Activation of the H + Pump in Elodea Leaves....Pages 205-213
Inhibition of ATP-Dependent Proton Pumping in Plant Tonoplast- and Plasmalemma-Enriched Fractions Caused by Mycochromone....Pages 215-218
Transport of Inorganic Ions in Tonoplast Vesicles....Pages 219-225
Wash-Activated Na + /H + Exchange in Tonoplast Vesicles from Beta Vulgaris Storage Root Disks....Pages 227-233
Isolation of Tonoplast Vesicles from Kiwi Fruit Cells: Electrochemical Investigation of K + and H + Membrane Transport Linked to a Magnesium-ATP Hydrolytic Activity....Pages 235-245
Some Characteristics of Tonoplast NO 3 − Transport Processes on Acer Pseudoplatanus L. Cells....Pages 247-253
Active Transport of Amino-Acids and Calcium Ions in Fungal Vacuoles....Pages 255-265
ATP-Dependent Uptake of Malate in Vacuoles from CAM Plant, Kalanchoe Daigremontiana ....Pages 267-274
Proton-Motive Force and H + /Amino-Acid Antiport at the Tonoplast of Riccia Fluitans Rhizoid Cells, Revealed by Direct Probing with the pH-Sensitive Microelectrode....Pages 275-279
Sugar Transport across the Tonoplast of Vacuoles Isolated from Protoplasts of Jerusalem Artichoke Tubers....Pages 281-286
Mg 2+ -ATP-Dependent Glucose Transport in Tonoplast Vesicles of Normal and Agrobacterium Tumefaciens -Transformed Tobacco Cells....Pages 287-289
Regulation of the UDP-Glucose Group Translocator by Some Cytoplasmic Components....Pages 291-295
Preliminary Evidence for Solubilization of an Active Tonoplast-Bound Vectorial Sucrose Synthesizing Enzyme Complex....Pages 297-300
Specific Uptake of the N-Oxides of Pyrrolizidine Alkaloids by Cells, Protoplasts and Vacuoles from Senecio Cell Cultures....Pages 301-304
Accumulation of Organic Solutes in Plant Vacuoles: The Interpretation of Data is Not so Easy....Pages 305-314
Vacuole Function in Neurospora ....Pages 315-324
 $$\\Delta {{\\overline {\\mu {\\text{H}}} }^{{\\text{ + }}}}$$ -Controlled Reversible Fluxes of H + and Calcium at the Tonoplast but Quasi-Total Citrate Sequestration within the Intact Vacuoles from the Latex Cells of Hevea Brasiliensis . Implications in the Production of Natural Rubber....Pages 325-338
Ajmalicine Transport into Vacuoles Isolated from Catharanthus Roseus Cells....Pages 339-347
Role of the Vacuole in Metal Ion Trapping as Studied by in Vivo 31 P-NMR Spectroscopy....Pages 349-359
Dynamics of Lysosomal Functions in Plant Vacuoles....Pages 361-368
Protein Degradation in Vacuoles from Acer Pseudoplatanus L. Cells....Pages 369-380
Protease Activity in Oat Leaf Vacuoles....Pages 381-382
Compartmentation of Glycosidases in a Light Vacuole Fraction from the Latex of Lactuca Sativa L.....Pages 383-391
The Possible Role of Endoplasmic Reticulum in the Biosynthesis and Transport of Anthocyanin Pigments....Pages 393-400
Patch Clamp Studies on the Transport of Ions Across the Membrane of Barley Vacuoles....Pages 401-406
Intracellular Distribution of Organic and Inorganic Anions in Mesophyll Cells: Transport Mechanisms in the Tonoplast....Pages 407-416
Vacuolar and Cytosolic Metabolite Pools by Comparative Fractionation of Vacuolate and Evacuolate Protoplasts....Pages 417-423
Subcellular Localization of Primary and Second Cardiac Glycosides, and the Related 16′-O-Glucosyl-Transferase in Cell Suspension Cultures of Digitalis Lanata ....Pages 425-433
Tonoplast: The Controlling Site for the in Vitro Solute Transport in Developing Soybean Cotyledons....Pages 435-439
The Vacuole in Relation to Plant Growth Regulators....Pages 441-447
Involvement of Vacuoles in Ethylene Metabolism in Plant Cells....Pages 449-454
The Vacuole: Possible Role in Signal Transduction Versus Cytoplasmic Homeostasis?....Pages 455-465
Transtonoplast pH Changes as a Signal for Activation of the Cytosolic Metabolism in Hevea Latex Cells in Response to Treatments with Ethrel (An Ethylene Releaser). Implications for the Production of a Secondary Metabolite: Natural Rubber....Pages 467-476
Production of Plant Secondary Metabolites by Plant Cell Cultures in Relation to the Site and Mechanism of Their Accumulation....Pages 477-484
Production of Secondary Metabolites in Cell Cultures of Some Terpenoid-Indole Alkaloids Producing Plants....Pages 485-494
Continuous Production of Indole Alkaloids by Gel-Entrapped Cells of Catharanthus Roseus ....Pages 495-501
Factors Affecting Alkaloid Production in Plant Cell Cultures....Pages 503-511
 Papaver Latex and Alkaloid Storage Vacuoles....Pages 513-528
Influence of External pH on Alkaloid Production and Excretion by Catharanthus Roseus Resting Cell Suspensions....Pages 529-533
Stress Metabolism in Plant Cell Cultures: Enhancement of Primary and Secondary Metabolites in Osmotically and Salt Adapted Cell Cultures of Catharanthus Roseus ....Pages 535-541
Significant Increase in Pyrimidinic Alkaloid (Trigonelline) Caused by Ionizing Radiation....Pages 543-548
Back Matter....Pages 549-562